This volume introduces the reader to the latest experimental and bioinformatics methods for DNA sequencing, RNA sequencing, cell-free tumour DNA sequencing, single cell sequencing, single-cell proteomics and metabolomics. Chapters detail advanced analysis methods, such as Genome-Wide Association Studies (GWAS), machine learning, reconstruction and analysis of gene regulatory networks and differential coexpression network analysis, and gave a practical guide for how to choose and use the right algorithm or software to handle specific high throughput data or multi-omics data. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Computational Systems Biology: Methods and Protocols aims to ensure successful results in the further study of this vital field.

This book argues that communication is at the heart of all approaches to dementia care, and is an in-depth exploration of ways of establishing and developing communication with people with dementia. It examines both the nature of dementia as a condition and the subjective experience of those affected. The authors consider in detail how communication between people with dementia and those who care for them changes, and how it can be maintained and enhanced. They include a significant amount of material quoted from people with dementia, and suggest ways of interpreting their words and actions. We learn about what it might be like to have dementia, and what sort of help is needed by people in this situation. Throughout the book the authors address the ethical issues and the implications for practice. "The combination of creativity and critical analysis which the joint authors as poet and psychologist bring to this book is especially productive...The interweaving of substantial practice examples based on conversations with people with dementia give persuasive authority to the careful exposition and detailed analysis. The book is much more than an exhortation to carers about how they should communicate. It challenges them to understand themselves and shows how they might use themselves to engage with people with dementia." - Faith Gibson, Emeritus Professor of Social Work Communication and the Care of People with Dementia is a key resource for students and professionals in health and social care work, including those in such fields as social work, nursing, occupational therapy, speech therapy, physiotherapy, clinical psychology, geriatric medicine, and the management of services.