This book provides basic information and different protocols associated with the Entomopathogenic nematodes (EPNs) and their symbiotic bacteria. Entomopathogenic nematodes (EPNs) of the genera Steinernema and Heterorhabditis and their associated bacterial symbionts Xenorhabdus and Photorhabdus aid nematode infective juveniles (IJs) in infecting and killing their insect hosts, creating a unique tripartite complex of host-vector-symbiont interactions. Due to this insect killing capability, EPNs are used as biological control agents of economically important insect pests. They are also a model system to study host-parasite interactions. It provides a systematic approach to various nematode procedures including pathogenicity, reproduction, foraging behaviour. It gives a brief outline on historical aspects, nematode-bacterium complex, biology and chemical ecology of EPNs. It concisely describes host insect rearing, nematode sampling and storage, isolation techniques, counting, handling and staining of nematodes, characterization including morphological, molecular and ecological studies, mass production, virulence bioassay, field application and efficacy. The book also includes methods and techniques for their associated symbiotic bacteria. This book serves as a laboratory manual and assists the readers to undertake advanced research in different aspects related to nematodes. It is useful for researchers in the fields of nematology, microbiology, bacteriology and entomology.

This book provides basic information and different protocols associated with the Entomopathogenic nematodes (EPNs) and their symbiotic bacteria. Entomopathogenic nematodes (EPNs) of the genera Steinernema and Heterorhabditis and their associated bacterial symbionts Xenorhabdus and Photorhabdus aid nematode infective juveniles (IJs) in infecting and killing their insect hosts, creating a unique tripartite complex of host-vector-symbiont interactions. Due to this insect killing capability, EPNs are used as biological control agents of economically important insect pests. They are also a model system to study host-parasite interactions. It provides a systematic approach to various nematode procedures including pathogenicity, reproduction, foraging behaviour. It gives a brief outline on historical aspects, nematode-bacterium complex, biology and chemical ecology of EPNs. It concisely describes host insect rearing, nematode sampling and storage, isolation techniques, counting, handling and staining of nematodes, characterization including morphological, molecular and ecological studies, mass production, virulence bioassay, field application and efficacy. The book also includes methods and techniques for their associated symbiotic bacteria. This book serves as a laboratory manual and assists the readers to undertake advanced research in different aspects related to nematodes. It is useful for researchers in the fields of nematology, microbiology, bacteriology and entomology.

This book provides basic information and different protocols associated with the Entomopathogenic nematodes (EPNs) and their symbiotic bacteria. Entomopathogenic nematodes (EPNs) of the genera Steinernema and Heterorhabditis and their associated bacterial symbionts Xenorhabdus and Photorhabdus aid nematode infective juveniles (IJs) in infecting and killing their insect hosts, creating a unique tripartite complex of host-vector-symbiont interactions. Due to this insect killing capability, EPNs are used as biological control agents of economically important insect pests. They are also a model system to study host-parasite interactions. It provides a systematic approach to various nematode procedures including pathogenicity, reproduction, foraging behaviour. It gives a brief outline on historical aspects, nematode-bacterium complex, biology and chemical ecology of EPNs. It concisely describes host insect rearing, nematode sampling and storage, isolation techniques, counting, handling and staining of nematodes, characterization including morphological, molecular and ecological studies, mass production, virulence bioassay, field application and efficacy. The book also includes methods and techniques for their associated symbiotic bacteria. This book serves as a laboratory manual and assists the readers to undertake advanced research in different aspects related to nematodes. It is useful for researchers in the fields of nematology, microbiology, bacteriology and entomology.

The volume deals with morphology, taxonomy and systematics of entomopathogenic nematodes (EPN) in the families Steinernematidae and Heterorhabditidae and bacteria associated with these nematodes. In the first part, history, taxonomic status, family and genus definitions of EPN are mentioned. In the second part, all useful aspects of morphology and methodology of EPN are mentioned with illustrations and SEM photographs and instructions on how to make the measurements, and how to use them in taxonomic work. A polychotomous key with SEM photographs and illustrations is provided. In the third part, full descriptions of all species are presented with latest information about each species. In the fourth part, molecular and phylogenetic methods for working with EPN are presented, including the latest information and instructions on how to use molecular data in taxonomic work. In the last part, bacteria associated with this group of nematodes are discussed in the context of the latest information about methodology, biology and taxon.

The editors of this book, who are world renown for their creativity with entomopathogenic nematodes, have assembled the foremost authorities from four continents to contribute on basic and applied concepts. The authors have taken advantage of this opportunity to express their views to a wide scientific audience. They have combined their international experience so that the latest developments in this fascinating and rapidly expanding field are present in comprehensive manner with diverse topics ranging from biological control theory to organismal and molecular biology.

In Advances in Entomopathogenic Nematode Taxonomy and Phylogeny the numerous species of Steinernema and Heterorhabditis described since the previous volume, published in 2007, are evaluated and discussed. Valid species proposed from 2007-2015 are covered in detail, each taxon having an illustrated diagnostic description and additional data on molecular characterisation, distribution and biology, etc. An addendum gives brief details of species proposed during 2016. An overview of the taxonomy of the two families provides an up-to-date list of species for both genera, including new synonyms and detailed commentary on specific status where appropriate. Tabular keys to all valid species of Steinernema and Heterorhabditis assist in diagnostics. A chapter on phylogeny and phylogeography completes the book.

This book describes entomopathogenic and slug parasitic nematodes as potential biocontrol agents in crop insect and slug pest management. Addressing research on these two nematodes from tropical, subtropical and temperate countries, it covers the new techniques and major developments regarding mass production, formulation, application, commercialization and safety measures. Plans for future strategies to make these beneficial nematodes cost-effective and expand their use by including them in integrated pest management programmes in different agro-ecosystems are also discussed. Biocontrol Agents: Entomopathogenic and Slug Parasitic Nematodes provides a comprehensive review of the topic and is an essential resource for researchers, industry practitioners and advanced students in the fields of biological control and integrated pest management.