Drug'DNA Interaction Protocols
Title | Drug'DNA Interaction Protocols PDF eBook |
Author | Keith R. Fox |
Publisher | Springer Science & Business Media |
Pages | 296 |
Release | 1997-10-07 |
Genre | Medical |
ISBN |
A collection of useful molecular techniques to illuminate and explore the interaction of drugs and ligands with DNA. These easily reproducible methods involve sequence recognition properties, as well as the physical approaches for measuring both the strength of interaction and the mode of drug binding to DNA. The interactions are also examined from a cellular perspective and for their usefulness in the design of new therapeutic agents. The powerful techniques detailed here will be particularly useful in elucidating the action of existing therapeutic agents, as well as in the design of new anti-cancer drugs with improved action and reduced toxicity.
Drug-DNA Interactions
Title | Drug-DNA Interactions PDF eBook |
Author | Kazuo Nakamoto |
Publisher | John Wiley & Sons |
Pages | 475 |
Release | 2008-09-08 |
Genre | Science |
ISBN | 0470369167 |
Learn vital information about drug-DNA interactions from Drug-DNA Interactions: Structures and Spectra, the only comprehensive book written about this topic. Understand the types of structural and bonding information that can be obtained using specific physico-chemical methods and discover how to design new drugs that are more effective than current treatments and have fewer side effects. Find detailed information about X-ray crystallography, NMR spectroscopy, molecular modeling, and optical spectroscopy such as UV-Visible absorption, fluorescence, circular dichroism (CD), flow linear dichroism (FLD), infrared (IR) and Raman spectroscopy.
Drug-Nucleic Acid Interactions
Title | Drug-Nucleic Acid Interactions PDF eBook |
Author | |
Publisher | Elsevier |
Pages | 740 |
Release | 2001-07-31 |
Genre | Medical |
ISBN | 0080496903 |
This volume consolidates the key methods for studying ligand-nucleic acid interactions into a convenient source. Techniques that are examined range from biophysical and chemical approaches to methods rooted in molecular and cell biology.
Spectroscopic Studies of Drug-DNA Interactions
Title | Spectroscopic Studies of Drug-DNA Interactions PDF eBook |
Author | Catherine Anna Butler |
Publisher | |
Pages | 452 |
Release | 1993 |
Genre | Antineoplastic agents |
ISBN |
Chaperonin Protocols
Title | Chaperonin Protocols PDF eBook |
Author | Christine Schneider |
Publisher | Springer Science & Business Media |
Pages | 212 |
Release | 2008-02-05 |
Genre | Science |
ISBN | 1592590616 |
Immunocytochemical Methods and Protocols
Title | Immunocytochemical Methods and Protocols PDF eBook |
Author | Lorette C. Javois |
Publisher | Springer Science & Business Media |
Pages | 928 |
Release | 2008-02-02 |
Genre | Science |
ISBN | 1592592139 |
Lorette Javois' timely new 2nd edition revises and updates her widely acclaimed collection of step-by-step immunocytochemical methods, one that is now used in many biological and biomedical research programs. The methods are designed for researchers and clinicians who wish to visualize molecules in plant or animal embryos, tissue sections, cells, or organelles. In addition to cutting-edge protocols for purifying and preparing antibodies, light microscopic analysis, confocal microscopy, FACS, and electron microscopy, this revised edition contains many new methods for applying immunocytochemical techniques in the clinical laboratory and in combination with in situ hybridization.
Natural Killer Cell Protocols
Title | Natural Killer Cell Protocols PDF eBook |
Author | Kerry S. Campbell |
Publisher | Springer Science & Business Media |
Pages | 394 |
Release | 2008-02-03 |
Genre | Medical |
ISBN | 1592590446 |
In Natural Killer Cell Protocols: Cellular and Molecular Methods, Kerry S. Campbell and Marco Colonna have assembled a comprehensive collection of readily reproducible methods designed to study natural killer (NK) cells from the broadest variety of viewpoints. These include not only classic techniques, but also new approaches to standard methods, newly evolved techniques that have become valuable for specific applications, and unique models for manipulating and studying NK cells. Among the advanced methods covered are those for in vitro transendothelial migration, in vivo detection of cells migrating into tumors, immunofluorescence staining of intracellular cytokines, and in vitro NK cell development. Valuable techniques for specific applications include vaccinia virus protein expression, soluble KIR-Fc fusions for HLA class I binding assays, calcium mobilization in cell conjugates, and identification of heterodimeric receptor complexes using cDNA library expression cloning. No less important are accounts of such classic methods as hybrid resistance, ADCC, viral defense, target cell cytotoxicity assays, cloning and culturing, tumor immunotherapy, and generation of HLA class I transfected target cells. Natural Killer Cell Protocols: Cellular and Molecular Methods offers immunologists, cancer researchers, virologists, and cell biologists today's most comprehensive collection of both established and cutting-edge techniques, methods that will contribute significantly to advancing our understanding of this fascinating and critically important class of cells.