Severely increasing anthropic pressure on natural water bodies sets a societal context where it is urgent to better understand the role of microbial biodiversity in aquatic ecosystem dynamics and resilience to perturbations. Micro-organisms regulate the chemical composition of the biosphere, influence climates, recycle nutrients, and decompose pollutants. The diversity of microbial communities and their ecological and metabolic functions are being explored in a variety of natural ecosystems, including extreme environments. Because of technical constraints, microbiologists have focused their efforts mainly on prokaryotes. In the environment, and particularly in aquatic ecosystems, the diversity and dynamics of microbial eukaryotes are still poorly understood. Among the new paradigms and challenges constantly emerging in aquatic sciences, probing the unexplored reservoir of novel ‘species’, genes, and metabolic pathways from microbial eukaryotes offers, perhaps, the most exciting research opportunity for the future. These were discussed during the topical session # 11 of the ASLO 2009 aquatic sciences meeting held at Nice, France, through a combination of invited and selected talks and posters. This book brings together part of these talks and presents contributions on ‘disregarded’ microbial eukaryote diversity and its ecological potential in aquatic ecosystems. We hope that this collection of papers will greatly expand our knowledge of the roles of eukaryotic microbes in aquatic ecosystems and will serve as a basis for significant research in the future. We believe that the decade to come promises exciting ecology on the interactions between microbial diversity, hidden genes and functions, and food web dynamics in aquatic systems.

Mycology has an integral role to play in the development of the biotechnology and biomedical sectors. It has become a subject of increasing importance as new fungi and their associated biomolecules are identified. As this discipline comes to the forefront of research in these sectors, the requirement for a consolidation of available research approaches is required. The First Edition of this book has a few basic and applied protocols. With the Second Edition, this book provides consolidated information on recent developments and the most widely used mycological methods available in the fields of biochemistry, biotechnology and microbiology. The methods outlined offer clear and concise directions to the reader and covers both standard protocols and more applied mycological methods. This book provides useful information for undergraduates, post-graduates, and specialists and researchers studying fungal biology.

This book highlights perspectives, insights, and data in the coupled fields of aquatic microbial ecology and biogeochemistry when viewed through the lens of collaborative duos – dual career couples. Their synergy and collaborative interactions have contributed substantially to our contemporary understanding of pattern, process and dynamics. This is thus a book by dual career couples about dual scientific processes. The papers herein represent wide-ranging topics, from the processes that structure microbial diversity to nitrogen and photosynthesis metabolism, to dynamics of changing ecosystems and processes and dynamics in individual ecosystems. In all, these papers take us from the Arctic to Africa, from the Arabian Sea to Australia, from small lakes in Maine and Yellowstone hot vents to the Sargasso Sea, and in the process provide analyses that make us think about the structure and function of all of these systems in the aquatic realm. This book is useful not only for the depth and breadth of knowledge conveyed in its chapters, but serves to guide dual career couples faced with the great challenges only they face. Great teams do make great science.

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This book discusses how aquatic microbial communities develop interactive metabolic coordination both within and between species to optimize their energetics. It explains that microbial community structuration often includes functional stratification among a multitude of organisms that variously exist either suspended in the water, lodged in sediments, or bound to one another as biofilms on solid surfaces. The authors describe techniques that can be used for preparing and distributing microbiologically safe drinking water, which presents the challenge of successfully removing the pathogenic members of the aquatic microbial community and then safely delivering that water to consumers. Drinking water distribution systems have their own microbial ecology, which we must both understand and control in order to maintain the safety of the water supply. Since studying aquatic microorganisms often entails identifying them, the book also discusses techniques for successfully isolating and cultivating bacteria. As such, it appeals to microbiologists, microbial ecologists and water quality scientists.

Next Generation Sequencing technologies are increasingly revealing that microbial taxa likely to be parasites or symbionts are probably much more prevalent and diverse than previously thought. Every well studied free-living species has parasites; parasites themselves can be parasitized. As a rule of thumb, there is an estimated 4 parasitic species for any given host, and the better a host is studied the more parasites are known to infect it. Therefore, parasites and other symbionts should represent a very large number of species and may far outnumber those with 'free-living' lifestyles. Paradoxically, free-living hosts, which form the bulk of our knowledge of biology, may be a minority! Microbial parasites typically are characterized by their small size, short generation time, and high rates of reproduction, with simple life cycle occurring generally within a single host. They are diverse and ubiquitous in the environment, comprising viruses, prokaryotes and eukaryotes. This Frontiers Research Topic sought to provide a broad overview but concise, comprehensive, well referenced and up-to-date state of the art for everyone involved with microbial parasites in aquatic microbial ecology.