Plant bacterial diseases are a major factor in economic crop losses worldwide.The transportation and use of clean, pathogen-free seed and propagative material is the most vital step in controlling bacterial diseases, but this requires accurate, rapid, sensitive, and reliable pathogen detection.The all-new second edition of Detection of Plant-Pathogenic Bacteria in Seed and Other Planting Material is an essential, easy-to-use lab manual describing and illustrating the latest methods for detecting a wide range of bacteria responsible for many of the world’s most economically significant bacterial plant diseases in crops.Yet this book does more than describe and illustrate the use of real-time PCR, rapid PCR-based assays, and other methods for specific, sensitive detection of bacteria in seed and propagative material. Its 48 chapters offer background information on the given bacteria and the diseases they cause, describe symptoms, detail their epidemiology, identify strategies for disease management, and provide protocols for detection in thorough detail.

The need for detection assays. Review of extraction and isolation methods. Detection and identification of bacteria. Detection of plant paghogenic bacteria in grain legumes and cereals. Detection of plant pathogenic bacteria in vegetables. Detection of plant pathogenic bacteria in vegetatively propagated crops.

Identification schemes; Gram-negative bacteria; Gram-positive bacteria; Cell wall-free prokaryotes.

Significant advancements have been made in pathogen detection technologies during the last decade. Indexing of plants and plant parts for the presence of specific pathogens has been most effective in some instances for avoiding and/or controlling disease. The new technologies for detecting low levels of pathogens will increase the value of indexing as a tool for plant disease control. Providing an overview of the status of detection technology, this volume is directed not only to scientists and students interested in detection technology, but also to those interested in formulating and implementing disease control and quarantine regulations. This book provides a conceptual framework which presents the current scientific literature, state-of-the-art assessments, and speculations on future developments and requirements of pathogen indexing methods. Chapters cover the different pathogen groups, review current practices in areas where detection technology has become important, and provide perspectives on how indexing technologies can be applied, how well it has worked, and which problems remain. Statistical treatment of detection limits, sampling strategies, risk assessment, cost, standardization, and quality control are also covered.

Rice seed health and quarantine; The rice plant and its environment; Equipment; Samples and sampling; dry seed inspection; Fungi; Bacteria; Nematodes; Viruses and mycoplasmalike organisms; Field inspection; Seed treatment; Weed seed; Insect pests; Fungal pathogens; Bacterial pathogens; Nematode pest; Organisms causing grain discoloration and damage.

This substantially updated edition now in full colour provides key techniques used when working with fungal and fungal-like plant pathogens. As a practical manual it also deals with disease recognition, detection and identification of fungi, plus methods to characterise and curate fungi and handle them under quarantine and quality assurance systems. Fungal Plant Pathogens: Applied Techniques, 2nd edition provides a valuable guide to investigating fungal plant diseases and interpreting laboratory findings for postgraduate and advanced undergraduate students, extension plant pathologists, consultants and advisers in agriculture, forestry and horticulture, and the food supply chain.