Data Analytics for Protein Crystallization
Title | Data Analytics for Protein Crystallization PDF eBook |
Author | Marc L. Pusey |
Publisher | Springer |
Pages | 245 |
Release | 2017-11-27 |
Genre | Computers |
ISBN | 3319589377 |
This unique text/reference presents an overview of the computational aspects of protein crystallization, describing how to build robotic high-throughput and crystallization analysis systems. The coverage encompasses the complete data analysis cycle, including the set-up of screens by analyzing prior crystallization trials, the classification of crystallization trial images by effective feature extraction, the analysis of crystal growth in time series images, the segmentation of crystal regions in images, the application of focal stacking methods for crystallization images, and the visualization of trials. Topics and features: describes the fundamentals of protein crystallization, and the scoring and categorization of crystallization image trials; introduces a selection of computational methods for protein crystallization screening, and the hardware and software architecture for a basic high-throughput system; presents an overview of the image features used in protein crystallization classification, and a spatio-temporal analysis of protein crystal growth; examines focal stacking techniques to avoid blurred crystallization images, and different thresholding methods for binarization or segmentation; discusses visualization methods and software for protein crystallization analysis, and reviews alternative methods to X-ray diffraction for obtaining structural information; provides an overview of the current challenges and potential future trends in protein crystallization. This interdisciplinary work serves as an essential reference on the computational and data analytics components of protein crystallization for the structural biology community, in addition to computer scientists wishing to enter the field of protein crystallization.
Protein Crystallization Strategies for Structural Genomics
Title | Protein Crystallization Strategies for Structural Genomics PDF eBook |
Author | Naomi E. Chayen |
Publisher | Internat'l University Line |
Pages | 300 |
Release | 2007 |
Genre | Science |
ISBN | 9780972077439 |
Protein Crystallography
Title | Protein Crystallography PDF eBook |
Author | Gwyndaf Evans |
Publisher | Royal Society of Chemistry |
Pages | 244 |
Release | 2018-06-21 |
Genre | Science |
ISBN | 1782627286 |
Protein crystallography has become vital to further understanding the structure and function of many complex biological systems. In recent years, structure determination has progressed tremendously however the quality of crystals and data sets can prevent the best results from being obtained. With contributions from world leading researchers whose software are used worldwide, this book provides a coherent approach on how to handle difficult crystallographic data and how to assess its quality. The chapters will cover all key aspects of protein crystallography, from instrumentation and data processing through to model building. This book also addresses challenges that protein crystallographers will face such as dealing with data from microcrystals and multi protein complexes. This book is ideal for both academics and researchers in industry looking for a comprehensive guide to protein crystallography.
Preparation and Analysis of Protein Crystals
Title | Preparation and Analysis of Protein Crystals PDF eBook |
Author | Alexander McPherson |
Publisher | John Wiley & Sons |
Pages | 424 |
Release | 1982-05-04 |
Genre | Science |
ISBN |
A complete guide to techniques and procedures for the preparation of proteins for crystallographic studies. Describes methods for protein crystallization; formation of isomorphous heavy atoms; x-ray diffraction and analysis; and photographic and computerbased data collection methods and instrumentation.
Crystals, X-rays and Proteins
Title | Crystals, X-rays and Proteins PDF eBook |
Author | Dennis Sherwood |
Publisher | OUP Oxford |
Pages | 640 |
Release | 2010-11-05 |
Genre | Science |
ISBN | 0191035521 |
A complete account of the theory of the diffraction of X-rays by crystals, with particular reference to the processes of determining the structures of protein molecules. This book is aimed primarily at structural biologists and biochemists but will also be valuable to those entering the field with a background in physical sciences or chemistry. It may be used at any post-school level, and develops from first principles all relevant mathematics, diffraction and wave theory, assuming no mathematical knowledge beyond integral calculus. The book covers a host of important topics in the area, including: - The practical aspects of sample preparation and X-ray data collection, using both laboratory and synchrotron sources - Data analysis at both theoretical and practical levels - The important role played by the Patterson function in structure analysis, by both molecular replacement and experimental phasing approaches - Methods for improving the resulting electron density map - The theoretical basis of methods used in refinement of protein crystal structures - In-depth explanation of the crucial task of defining the binding sites of ligands and drug molecules - The complementary roles of other diffraction methods: these reveal further detail of great functional importance in a crystal structure.
Practical Protein Crystallography
Title | Practical Protein Crystallography PDF eBook |
Author | Duncan E. McRee |
Publisher | Academic Press |
Pages | 403 |
Release | 2012-12-02 |
Genre | Science |
ISBN | 0323138888 |
Designed for easy use by both new and experienced protein crystallographers, this much-needed book is for anyone interested in solving protein structures by the method of crystallography. It contains many examples ofactual experiments and data, including electron density maps. Computer methods and computer code samples are presented. Practical Protein Crystallography is loaded with new information on area detectors, synchrotron radiation techniques, and the latest computer methods, and features the XtalView software system. Graduate students and teachers in physical biochemistry and pharmaceutical researchers will find this text a timely and convenient aid.
Protein Crystallization
Title | Protein Crystallization PDF eBook |
Author | Terese M. Bergfors |
Publisher | Internat'l University Line |
Pages | 505 |
Release | 2009 |
Genre | Medical |
ISBN | 0972077448 |