Topics covered in this book include RNA silencing and its suppression in plant virus infection, virus replication mechanisms, the association of cellular membranes with virus replication and movement, plant genetic resistance to viruses, viral cell-to-cell spread, long distance movement in plants, virus induced ER stress, virus diversity and evolution, virus-vector interactions, cross protection, geminiviruses, negative strand RNA viruses, viroids, and the diagnosis of plant viral diseases using next generation sequencing. This book was anticipated to help plant pathologists, scholars, professors, teachers and advanced students in the field with a comprehensive state-of-the-art knowledge of the subject.

In calling this series Molecular Plant Virology, I had in mind aspects of plant virology of interest to biochemists, molecular geneticists, biophysicists, genetic engineers, or, collectively, �molecular biologists�. At the same time, the intention was to provide up-to-date reviews, by expert contributors, on current research topics in plant virology of interest and referential use to virologists and plant biologists. The selected topics are pitched mainly at a research level, but with sufficient introduction and cross-referencing to enable graduate students to enter this fascinating field and, hopefully, not get lost.

Vector transmission of pathogens affecting human, animal, and plant health continues to plague mankind both in industrialized and Third World coun tries. The diseases caused by these pathogens cost billions of dollars an nually in medical expenses and lost productivity. Some cause widespread of food-and fiber-producing plants and animals, whereas others destruction present direct and immediate threats to human life and further development in Third World countries. During the past 15 years or so, we have witnessed an explosive increase in interest in how vectors acquire, carry, and subsequently inoculate dis ease agents to human, animal, and plant hosts. This interest transcends the boundaries of anyone discipline and involves researchers from such varied fields as human and veterinary medicine, entomology, plant pa thology, virology, physiology, microbiology, parasitology, biochemistry, molecular biology, genetic engineering, ultrastructure, biophysics, bio systematics, biogeography, ecology, behavioral sciences, and others. Ac companying and perhaps generating this renewed interest is the realization that fundamental knowledge of pathogen-vector-host interrelationships is a first and necessary step in our quest for efficient, safe methods of disease control.

The Encyclopedia of Plant Viruses and Viroids provides an up-to-date information on the viruses and viroids infecting all types of cultivated and weed plants at global level; and is unique among plant virology texts as it is organized alphabetically by the genus name of the host plant infected. It allows the reader to readily determine all of the different viruses currently reported naturally infecting the plant genus and species. Information is provided for each virus and viroids on common synonyms, current taxonomic status, geographical distribution, symptoms induced, other known hosts, means of transmission and properties of both the virus particles and the genome. Where ever the same virus is known to infect multiple hosts, host-specific information, biological properties and genome characters are presented under each host affected. The index can be utilized to identify other crops infected by the same virus, showing which other crops might be at risk of infection in the event of introduction of a virus that has not previously reported in an area, or which might serve as potential virus reservoirs for infection of more sensitive or economically important crops than the host in which it is initially identified. The taxonomy and nomenclature of the viruses and viroids are followed based on the current guidelines of the 10th ICTV Report, covering up to 2018 and also about many new viruses and viroids that have been reported but not yet recognized as species by ICTV, have been included in this Encyclopedia. The uniqueness of this Encyclopedia is that all the known viruses and viroids affecting more than 1010 plant species at global level are described and the plant species are arranged in alphabetical order of the scientific name of the plant along the relevant information on 1518 viruses and viroids and is the ready-reckoner of the global plant species and their viruses and viroids for students, scientists, teachers of Plant Pathology& Virology; and also for the crop protection professionals, agricultural policymakers, seed companies and quarantine agencies.

This volume discusses traditional and current techniques that are successfully used to diagnose plant viruses and study molecular plant-virus interactions. The chapters in this book cover topics such as in vivo detection of double-stranded RNA, developing rice mutant using CRISPR-Cas9-based technology, protein-protein interaction assays, purification and transfection of protoplasts, protocols for gene silencing, and transmission electron microscopy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and practical, Plant Virology: Methods and Protocols is a valuable resource for plant pathologists, microbiologists, virologists, graduate students, and teachers who are interested in learning more about the developments in plant virology research.

In calling this series Molecular Plant Virology, I had in mind aspects of plant virology of interest to biochemists, molecular geneticists, biophysicists, genetic engineers, or, collectively,molecular biologists. At the same time, the intention was to provide up-to-date reviews, by expert contributors, on current research topics in plant virology of interest and referential use to virologists and plant biologists. The selected topics are pitched mainly at a research level, but with sufficient introduction and cross-referencing to enable graduate students to enter this fascinating field and, hopefully, not get lost.

In calling this series Molecular Plant Virology, I had in mind aspects of plant virology of interest to biochemists, molecular geneticists, biophysicists, genetic engineers, or, collectively,molecular biologists. At the same time, the intention was to provide up-to-date reviews, by expert contributors, on current research topics in plant virology of interest and referential use to virologists and plant biologists. The selected topics are pitched mainly at a research level, but with sufficient introduction and cross-referencing to enable graduate students to enter this fascinating field and, hopefully, not get lost.