This volume contains shortened versions of the methods published in the looseleaf manual Current Protocols in Molecular Biology. It presents fully-tested, current techniques based on material from the core manual and from the quarterly update service. Includes all step-by-step descriptions of methods covered in the first ten chapters of CPMB and provides enough detail to perform the experiments (only introductions, annotations, and commentary have been omitted). Marginal notes explain the hows and whys of many steps, and provide tips on safety, storage, and anticipated results. Includes references and recipes for all reagents and media and helpful tables and illustrations.