All areas of high performance liquid chromatography (HPLC) relevant to the separation of amino acid and polypeptide samples are described in these comprehensive volumes. Eighty-four chapters cover the application of HPLC to research in the life sciences. Both basic information and specific details are presented on such areas as commercially available HPLC columns, ligand-exchange chromatography, peptide hormone separations, peptide antibiotic separations and drug-protein binding. Practical suggestions are given to help you avoid pitfalls which can exist in the analysis of biological materials.

Introduction to the general principles of HPLC; HPLC instrumentation; Mobile phases; Detection methods; Separation of free amino acids; Resolution of amino acids as diastereomeric derivatives.

Introduction to the general principles of HPLC; HPLC instrumentation; Mobile phases; Detection methods; Separation of free amino acids; Resolution of amino acids as diastereomeric derivatives.