A contemporary guide to the diagnostic principles and practices of immunology and serology in the clinical laboratory.

A contemporary guide to the diagnostic principles and practices of immunology and serology in the clinical laboratory.

The fourth edition of The Immunoassay Handbook provides an excellent, thoroughly updated guide to the science, technology and applications of ELISA and other immunoassays, including a wealth of practical advice. It encompasses a wide range of methods and gives an insight into the latest developments and applications in clinical and veterinary practice and in pharmaceutical and life science research. Highly illustrated and clearly written, this award-winning reference work provides an excellent guide to this fast-growing field. Revised and extensively updated, with over 30% new material and 77 chapters, it reveals the underlying common principles and simplifies an abundance of innovation. The Immunoassay Handbook reviews a wide range of topics, now including lateral flow, microsphere multiplex assays, immunohistochemistry, practical ELISA development, assay interferences, pharmaceutical applications, qualitative immunoassays, antibody detection and lab-on-a-chip. This handbook is a must-read for all who use immunoassay as a tool, including clinicians, clinical and veterinary chemists, biochemists, food technologists, environmental scientists, and students and researchers in medicine, immunology and proteomics. It is an essential reference for the immunoassay industry. Provides an excellent revised guide to this commercially highly successful technology in diagnostics and research, from consumer home pregnancy kits to AIDS testing.www.immunoassayhandbook.com is a great resource that we put a lot of effort into. The content is designed to encourage purchases of single chapters or the entire book. David Wild is a healthcare industry veteran, with experience in biotechnology, pharmaceuticals, medical devices and immunodiagnostics, which remains his passion. He worked for Amersham, Eastman-Kodak, Johnson & Johnson, and Bristol-Myers Squibb, and consulted for diagnostics and biotechnology companies. He led research and development programs, design and construction of chemical and biotechnology plants, and integration of acquired companies. Director-level positions included Research and Development, Design Engineering, Operations and Strategy, for billion dollar businesses. He retired from full-time work in 2012 to focus on his role as Editor of The Immunoassay Handbook, and advises on product development, manufacturing and marketing. - Provides a unique mix of theory, practical advice and applications, with numerous examples - Offers explanations of technologies under development and practical insider tips that are sometimes omitted from scientific papers - Includes a comprehensive troubleshooting guide, useful for solving problems and improving assay performancee - Provides valuable chapter updates, now available on www.immunoassayhandbook.com

Phlebotomy Essentials, Eighth Edition provides accurate, up-to-date, and practical information and instruction in phlebotomy procedures and techniques, along with a comprehensive background in phlebotomy theory and principles. It is appropriate for use as an instructional text or as a reference for those who wish to update skills or study for national certification. Enhanced with new images, a more efficient design, and new contributions from leading subject matter experts, this updated edition details how today's phlebotomists work in an approach optimized for how today's students learn. Combined with an optional Workbook, Exam Review book, and updated digital courseware, the latest edition of Phlebotomy Essentials represents a cornerstone of preparation for a successful career in phlebotomy.

Immunodiagnostic tests are analytical methods that use antibodies as reagents whose results are used to aid diagnosis and are widely used in many scientific disciplines and in many different ways. Perhaps the most widespread and obvious use is in clinical applications, but immunodiagnostic tests are also used in other fields such as forensic science and environmental and food analysis. The different types of test range from simple manual methods to fully automated systems with sophisticated integrated detection. Immunodiagnostics: A Practical Approach starts off by explaining the principles and development of immunodiagnostic tests, specifically the use of radioisotopes as tracers. Chapter 2 explains the use of solid-phase supports to bind immunoreagents. Enzymes are widely used as labels in immunoassays and their use with colourimetric, fluorimetric, and chemiluminescent detection systems is described. The use of enzymes as labels reflects the move away from radioisotopes and one of the most powerful non- radioisotpoc prodcedures is the time-resolved fluorescence assay. Enzymes can also be used as a simple method of obtaining high performance from immunodiagnostics and this application is covered later in the book. The next set of techniques to be described are light scattering techniques, which can be used in either simple manual assays or in sophisticated automated procedures. The penultimate chapter describes the principles of automation of immunodiagnostic tests. The last topic to be discussed is that of quality assurance.

Principles and Practice of Pediatric Infectious Disease provides the comprehensive and actionable coverage you need to understand, diagnose, and manage the ever-changing, high-risk clinical problems caused by pediatric infectious diseases. With new chapters, expanded and updated coverage, and increased worldwide perspectives, this authoritative medical reference offers the latest need-to-know information in an easily-accessible, high-yield format for quick answers and fast, effective intervention! Spend less time searching thanks to a consistent, easily-accessible format featuring revised high-yield information boxes, highlighted key points, and an abundance of detailed illustrations and at-a-glance tables. Be prepared for the unexpected! A veritable "who's who" of global authorities provides practical knowledge to effectively diagnose and manage almost any infectious disease you may encounter. Quickly look up the answers you need by clinical presentation, pathogen, or type of host. Get expanded coverage for all types of infectious diseases including new chapters on infection related to pets and exotic animals, and tickborne infections. Apply the latest recommendations and treatments for emerging and re-emerging diseases including the H1N1 virus.

The underlying technology and the range of test parameters available are evolving rapidly. The primary advantage of POCT is the convenience of performing the test close to the patient and the speed at which test results can be obtained, compared to sending a sample to a laboratory and waiting for results to be returned. Thus, a series of clinical applications are possible that can shorten the time for clinical decision-making about additional testing or therapy, as delays are no longer caused by preparation of clinical samples, transport, and central laboratory analysis. Tests in a POC format can now be found for many medical disciplines including endocrinology/diabetes, cardiology, nephrology, critical care, fertility, hematology/coagulation, infectious disease and microbiology, and general health screening. Point-of-care testing (POCT) enables health care personnel to perform clinical laboratory testing near the patient. The idea of conventional and POCT laboratory services presiding within a hospital seems contradictory; yet, they are, in fact, complementary: together POCT and central laboratory are important for the optimal functioning of diagnostic processes. They complement each other, provided that a dedicated POCT coordination integrates the quality assurance of POCT into the overall quality management system of the central laboratory. The motivation of the third edition of the POCT book from Luppa/Junker, which is now also available in English, is to explore and describe clinically relevant analytical techniques, organizational concepts for application and future perspectives of POCT. From descriptions of the opportunities that POCT can provide to the limitations that clinician’s must be cautioned about, this book provides an overview of the many aspects that challenge those who choose to implement POCT. Technologies, clinical applications, networking issues and quality regulations are described as well as a survey of future technologies that are on the future horizon. The editors have spent considerable efforts to update the book in general and to highlight the latest developments, e.g., novel POCT applications of nucleic acid testing for the rapid identification of infectious agents. Of particular note is also that a cross-country comparison of POCT quality rules is being described by a team of international experts in this field.