Characterization of Human Induced Pluripotent Stem Cell-derived Cardiomyocytes and Human Primary Cardiomyocytes on the Single-cell Level
Title | Characterization of Human Induced Pluripotent Stem Cell-derived Cardiomyocytes and Human Primary Cardiomyocytes on the Single-cell Level PDF eBook |
Author | Christina Schmid |
Publisher | |
Pages | |
Release | 2021 |
Genre | |
ISBN |
Calcium Handling in hiPSC-Derived Cardiomyocytes
Title | Calcium Handling in hiPSC-Derived Cardiomyocytes PDF eBook |
Author | Lee Yee-Ki |
Publisher | Springer Science & Business Media |
Pages | 63 |
Release | 2012-06-21 |
Genre | Science |
ISBN | 1461440939 |
Calcium is crucial in governing contractile activities of myofilaments in cardiomyocytes, any defeats in calcium homeostasis of the cells would adversely affect heart pumping action. The characterization of calcium handling properties in human induced pluripotent stem cell-derived cardiomyocytes (iPS-CMCs) is of significant interest and pertinent to the stem cell and cardiac regenerative field because of their potential patient-specific therapeutic use.
Functional Characterization of Cardiomyocytes Derived from Human Induced Pluripotent Stem Cells
Title | Functional Characterization of Cardiomyocytes Derived from Human Induced Pluripotent Stem Cells PDF eBook |
Author | Xiaowu Sheng |
Publisher | |
Pages | 61 |
Release | 2012 |
Genre | |
ISBN |
Pluripotent Stem-Cell Derived Cardiomyocytes
Title | Pluripotent Stem-Cell Derived Cardiomyocytes PDF eBook |
Author | Yoshinori Yoshida |
Publisher | |
Pages | 304 |
Release | 2021 |
Genre | Cardiovascular system |
ISBN | 9781071614846 |
This volume provides methodologies for ES and iPS cell technology on the study of cardiovascular diseases. Chapters guide readers through protocols on cardiomyocyte generation from pluripotent stem cells, physiological measurements, bioinformatic analysis, gene editing technology, and cell transplantation studies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Pluripotent Stem-Cell Derived Cardiomyocytes aims to help researchers set up experiments using pluripotent stem cell-derived cardiac cells.
Biochemical Characterization of Induced Pluripotent Stem Cell-derived Cardiomyocytes as a Model of Barth Syndrome
Title | Biochemical Characterization of Induced Pluripotent Stem Cell-derived Cardiomyocytes as a Model of Barth Syndrome PDF eBook |
Author | Alisha J. House |
Publisher | |
Pages | 0 |
Release | 2022 |
Genre | |
ISBN |
Barth Syndrome (BTHS) is an X-linked inborn error of metabolism (IEM) which manifests as a multi-systemic disease. One of the primary symptoms is dilated cardiomyopathy, and alongside the cardiovascular disease that arises, patients often experience metabolic abnormalities such as 3-methylglutaconic aciduria, growth retardation, and neutropenia. There has been a need for the development of a suitable in vitro modeling system which will accurately recapitulate the biochemical and physical nature of BTHS. The purpose of this project has been to develop a model for studying the biochemical pathogenesis of Barth Syndrome using human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs). To achieve this, groundwork was laid in developing differentiation methods for producing ventricular hiPSC-CMs from hiPSCs. Simultaneously, a group of methods utilizing mass spectrometry in the biochemical analysis of hiPSC-CMs was developed, and this has provided a novel platform for the detection of many metabolites for the purpose of analyzing the metabolic abnormalities due to BTHS. Biochemical characterization of hiPSC-CMs is a novel tool to be used in assessing biochemical abnormalities and has not previously been achieved.
Pluripotent Stem-Cell Derived Cardiomyocytes
Title | Pluripotent Stem-Cell Derived Cardiomyocytes PDF eBook |
Author | Yoshinori Yoshida |
Publisher | Humana |
Pages | 304 |
Release | 2022-08-08 |
Genre | Science |
ISBN | 9781071614860 |
This volume provides methodologies for ES and iPS cell technology on the study of cardiovascular diseases. Chapters guide readers through protocols on cardiomyocyte generation from pluripotent stem cells, physiological measurements, bioinformatic analysis, gene editing technology, and cell transplantation studies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Pluripotent Stem-Cell Derived Cardiomyocytes aims to help researchers set up experiments using pluripotent stem cell-derived cardiac cells.
Cardiac Regeneration using Stem Cells
Title | Cardiac Regeneration using Stem Cells PDF eBook |
Author | Keiichi Fukuda |
Publisher | CRC Press |
Pages | 374 |
Release | 2013-04-10 |
Genre | Medical |
ISBN | 1466578408 |
To achieve cardiac regeneration using pluripotent stem (iPS) cells, researchers must understand iPS cell generation methods, cardiomyocyte differentiation protocols, cardiomyocyte characterization methods, and tissue engineering. This book presents the current status and future possibilities in cardiac regeneration using iPS cells. Written by top r