Second-harmonic generation (SHG) microscopy has shown great promise for imaging live cells and tissues, with applications in basic science, medical research, and tissue engineering. Second Harmonic Generation Imaging offers a complete guide to this optical modality, from basic principles, instrumentation, methods, and image analysis to biomedical a

This detailed collection serves as a unique and excellent collection of state-of-the-art methods and protocols to interrogate cell migration in a wide variety of different contexts and model organisms, as well as advanced image analysis and quantitative assessment of a diverse array of parameters related to cell migration. The book focuses on the cell biology of cell migration, developmental model systems to assess cell migration during morphogenesis, cell migration in cancers and the tumor micro-environment, as well as blood vessel formation and interactions. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Cell Migration in Three Dimensions provides a solid foundation for scientists of different disciplines to investigate cell migration in biological processes. Chapters 7, 12, 16, 17, 19, 22, and 24 are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Mechanobiology of Cell-Matrix Interactions focuses on characterization and modeling of interactions between cells and their local extracellular environment, exploring how these interactions may mediate cell behavior. Studies of cell-matrix interactions rely on integrating engineering, (molecular and cellular) biology, and imaging disciplines. Recent advances in the field have begun to unravel our understanding of how cells gather information from their surrounding environment, and how they interrogate such information during the cell fate decision making process. Topics include adhesive and integrin-ligand interactions; extracellular influences on cell biology and behavior; cooperative mechanisms of cell-cell and cell-matrix interactions; the mechanobiology of pathological processes; (multi-scale) modeling approaches to describe the complexity or cell-matrix interactions; and quantitative methods required for such experimental and modeling studies.

Mathematical modeling, analysis and simulation are set to play crucial roles in explaining tumor behavior, and the uncontrolled growth of cancer cells over multiple time and spatial scales. This book, the first to integrate state-of-the-art numerical techniques with experimental data, provides an in-depth assessment of tumor cell modeling at multiple scales. The first part of the text presents a detailed biological background with an examination of single-phase and multi-phase continuum tumor modeling, discrete cell modeling, and hybrid continuum-discrete modeling. In the final two chapters, the authors guide the reader through problem-based illustrations and case studies of brain and breast cancer, to demonstrate the future potential of modeling in cancer research. This book has wide interdisciplinary appeal and is a valuable resource for mathematical biologists, biomedical engineers and clinical cancer research communities wishing to understand this emerging field.

Cell migration is a key component of many biological processes including embryonic development, immune responses, wound healing, organ regeneration, and cancer cell metastasis, thus making it an exciting and crucial field of study. The aim of Cell Migration: Developmental Methods and Protocols, Second Edition is to bring together a wide range of these techniques from the more basic migration assays, which are still the foundation of many cell migration studies, to state-of-the-art techniques and recent technical advances. Divided into three convenient parts, the volume begins with a number of basic in vitro migration assays including measurements of wound healing, cell scattering, invasion, and chemotaxis, as well as more complex measurements of transendothelial migration, the use of microfluidic chambers, and imaging cell migration in 3D. It continues with procedures for the imaging and measurement of cell migration in vivo including protocols for the use of chick, drosophila, and zebrafish embryos, and methods to measure metastatic spread and angiogenesis in mice, then concludes with a vital section on emerging techniques in the cell migration field including the use of TIRF, FRAP, and FRET microscopy. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes from the experts on troubleshooting and avoiding known pitfalls. Comprehensive and up-to-date, Cell Migration: Developmental Methods and Protocols, Second Edition provides a comprehensive catalogue of techniques for the study of cell migration that can be used as a useful reference source for any researcher who wishes to explore this significant area of cell biology.

Explores a Range of Multiscale Biomechanics/Mechanobiology ConceptsCell and Matrix Mechanics presents cutting-edge research at the molecular, cellular, and tissue levels in the field of cell mechanics. This book involves key experts in the field, and covers crucial areas of cell and tissue mechanics, with an emphasis on the roles of mechanical forc

Biology and Engineering of Stem Cell Niches covers a wide spectrum of research and current knowledge on embryonic and adult stem cell niches, focusing on the understanding of stem cell niche molecules and signaling mechanisms, including cell-cell/cell-matrix interactions. The book comprehensively reviews factors regulating stem cell behavior and the corresponding approaches for understanding the subsequent effect of providing the proper matrix molecules, mechanical cues, and/or chemical cues. It encompasses a variety of tools and techniques for developing biomaterials-based methods to model synthetic stem cell niches in vivo, or to enhance and direct stem cell fate in vitro. A final section of the book discusses stem cell niche bioengineering strategies and current advances in each tissue type. - Includes the importance of Cell-Cell and Cell Matrix Interactions in each specific tissue and system - Authored and edited by authorities in this emerging and multidisciplinary field - Includes valuable links to 5-10 minute YouTube© author videos that describe main points