2+ The regulation of intracellular Ca is a common theme presented in many 2+ papers over the last 20 or so years, and the description of the Ca -sensitive indicator dye fura 2 in 1985 resulted in a massive increase in these types of 2+ studies. Aspects of the regulation of intracellular Ca have been dealt with in many of the subsequent chapters and will therefore not be covered again. Calcium Signaling Protocols results from a chance discussion with Dr. R. I. Norman of the Department of Medicine at Leicester University and r- resents a major effort from a group of extremely helpful and very patient - thors. Putting a book like this together takes time and I am indebted to these authors without whom this project would have remained a chance discussion. I am also very grateful to Professor J. M. Walker, the series editor, for all his help and advice over the course of this project and particularly his help editing the first batch of chapters. I would also like to thank Dr E. L. Pallett for help and advice regarding interconversion of Mac and Word files and for archiving chapters.

Calcium Entry Channels in Non-Excitable Cells focuses on methods of investigating the structure and function of non-voltage gated calcium channels. Each chapter presents important discoveries in calcium entry pathways, specifically dealing with the molecular identification of store-operated calcium channels which were reviewed by earlier volumes in the Methods in Signal Transduction series. Crystallographic and pharmacological approaches to the study of calcium channels of epithelial cells are also discussed. Calcium ion is a messenger in most cell types. Whereas voltage gated calcium channels have been studied extensively, the non-voltage gated calcium entry channel genes have only been identified relatively recently. The book will fill this important niche.

T cells play a vital role mediating adaptive immunity, a specific acquired resistance to an infectious agent produced by the introduction of an antigen. There are a variety of T cell types with different functions. They are called T cells, because they are derived from the thymus gland. This volume discusses how T cells are regulated through the operation of signaling mechanisms. Topics covered include positive and negative selection, early events in T cell receptor engagement, and various T cell subsets.

The first edition of James Putney's Calcium Signaling offered readers a comprehensive view of the fascinating diversity of technologies that the new field of calcium signaling employed. And while that work is still regarded as a premier text on the basics of calcium signaling, progress has been so dramatic that an update is now required. In Cal

This new edition focuses on the applications of fluorescent and bioluminescent techniques for studying calcium signalling and calcium dependent processes. Cameleon probes, targeting bioluminescent probes, low affinity fluorescence indicators and chelators, and photometric techniques are all covered in depth. Also described in detail are methods for monitoring mitochondrial function, measuring calcium in the nuclear envelope and nucleoplasm, measuring calcium dependent gene expression, monitoring calcium signals in multicellular preparations, and measuring calcium extrusion. A major problem of fluorescent calcium measurements is the artifacts from zinc and transition metals and a chapter is devoted to their detection and minimization. Some of the pharmacological methods of studying calcium release are also covered. With many of the chapters written by the very scientists who developed the methods they are discussing this book is the most up-to-date, invaluable book for those studying cellular calcium.