Rooted in thousands of pages of Access to Information documents and dozens of interviews carried out throughout Latin America, Blood of Extraction examines the increasing presence of Canadian mining companies in Latin America and the environmental and human rights abuses that have occurred as a result. By following the money, Gordon and Webber illustrate the myriad ways Canadian-based multinational corporations, backed by the Canadian state, have developed extensive economic interests in Latin America over the last two decades at the expense of Latin American people and the environment. Latin American communities affected by Canadian resource extraction are now organized into hundreds of opposition movements, from Mexico to Argentina, and the authors illustrate the strategies used by the Canadian state to silence this resistance and advance corporate interests.

Written in Blood features the work of Appalachia’s leading scholars and activists making available an accurate, ungilded, and uncensored understanding of our history. Combining new revelations from the past with sketches of a sane path forward, this is a deliberate collection looking at our past, present, and future. Sociologist Wess Harris (When Miners March) further documents the infamous Esau scrip system for women, suggesting an institutionalized practice of forced sexual servitude that was part of coal company policy. In a conversation with award-winning oral historian Michael Kline, federal mine inspector Larry Layne explains corporate complicity in the 1968 Farmington Mine disaster which killed seventy-eight men and became the catalyst for the passage of major changes in U.S. mine safety laws. Mine safety expert and whistleblower Jack Spadaro speaks candidly of years of attempts to silence his courageous voice and recalls government and university collaboration in covering up details of the 1972 Buffalo Creek flooding disaster, which killed over a hundred people and left four thousand homeless. Moving to the next generation of thinkers and activists, attorney Nathan Fetty examines current events in Appalachia and musician Carrie Kline suggests paths forward for people wishing to set their own course rather than depend on the kindness of corporations.

In this new edition, the editors have thoroughly updated and dramatically expanded the number of protocols to take advantage of the newest technologies used in all branches of research and clinical medicine today. These proven methods include real time PCR, SNP analysis, nested PCR, direct PCR, and long range PCR. Among the highlights are chapters on genome profiling by SAGE, differential display and chip technologies, the amplification of whole genome DNA by random degenerate oligonucleotide PCR, and the refinement of PCR methods for the analysis of fragmented DNA from fixed tissues. Each fully tested protocol is described in step-by-step detail by an established expert in the field and includes a background introduction outlining the principle behind the technique, equipment and reagent lists, tips on trouble shooting and avoiding known pitfalls, and, where needed, a discussion of the interpretation and use of results.

During the last decade, the South American continent has seen a strong push for transnational integration, initiated by the former Brazilian president Fernando Henrique Cardoso, who (with the endorsement of eleven other nations) spearheaded the Initiative for the Integration of Regional Infrastructure in South America (IIRSA), a comprehensive energy, transport, and communications network. The most aggressive transcontinental integration project ever planned for South America, the initiative systematically deploys ten east-west infrastructural corridors, enhancing economic development but raising important questions about the polarizing effect of pitting regional needs against the colossal processes of resource extraction. Providing much-needed historical contextualization to IIRSA’s agenda, Beyond the City ties together a series of spatial models and offers a survey of regional strategies in five case studies of often overlooked sites built outside the traditional South American urban constructs. Implementing the term “resource extraction urbanism,” the architect and urbanist Felipe Correa takes us from Brazil’s nineteenth-century regional capital city of Belo Horizonte to the experimental, circular, “temporary” city of Vila Piloto in Três Lagoas. In Chile, he surveys the mining town of María Elena. In Venezuela, he explores petrochemical encampments at Judibana and El Tablazo, as well as new industrial frontiers at Ciudad Guayana. The result is both a cautionary tale, bringing to light a history of societies that were “inscribed” and administered, and a perceptive examination of the agency of architecture and urban planning in shaping South American lives.

Phlebotomy uses large, hollow needles to remove blood specimens for lab testing or blood donation. Each step in the process carries risks - both for patients and health workers. Patients may be bruised. Health workers may receive needle-stick injuries. Both can become infected with bloodborne organisms such as hepatitis B, HIV, syphilis or malaria. Moreover, each step affects the quality of the specimen and the diagnosis. A contaminated specimen will produce a misdiagnosis. Clerical errors can prove fatal. The new WHO guidelines provide recommended steps for safe phlebotomy and reiterate accepted principles for drawing, collecting blood and transporting blood to laboratories/blood banks.

This presentation describes various aspects of the regulation of tissue oxygenation, including the roles of the circulatory system, respiratory system, and blood, the carrier of oxygen within these components of the cardiorespiratory system. The respiratory system takes oxygen from the atmosphere and transports it by diffusion from the air in the alveoli to the blood flowing through the pulmonary capillaries. The cardiovascular system then moves the oxygenated blood from the heart to the microcirculation of the various organs by convection, where oxygen is released from hemoglobin in the red blood cells and moves to the parenchymal cells of each tissue by diffusion. Oxygen that has diffused into cells is then utilized in the mitochondria to produce adenosine triphosphate (ATP), the energy currency of all cells. The mitochondria are able to produce ATP until the oxygen tension or PO2 on the cell surface falls to a critical level of about 4–5 mm Hg. Thus, in order to meet the energetic needs of cells, it is important to maintain a continuous supply of oxygen to the mitochondria at or above the critical PO2 . In order to accomplish this desired outcome, the cardiorespiratory system, including the blood, must be capable of regulation to ensure survival of all tissues under a wide range of circumstances. The purpose of this presentation is to provide basic information about the operation and regulation of the cardiovascular and respiratory systems, as well as the properties of the blood and parenchymal cells, so that a fundamental understanding of the regulation of tissue oxygenation is achieved.

The new WHO guidelines provide recommended steps for safe phlebotomy and reiterate accepted principles for drawing, collecting blood and transporting blood to laboratories/blood banks. The main areas covered by the toolkit are: 1. bloodborne pathogens transmitted through unsafe injection practices;2. relevant elements of standard precautions and associated barrier protection;3. best injection and related infection prevention and control practices;4. occupational risk factors and their management.