The book is structured in nine sections, each containing several chapters. The volume starts with an overview of analytical techniques and progresses through purification of proteins; protein modification and inactivation; protein size, shape, and structure; enzyme kinetics; protein-ligand interactions; industrial enzymology; and laboratory quality control. The book is targeted at all scientists interested in protein research.

In the post-genomic world, advances in the comprehension of cell behaviour will depend upon scientists deciphering the molecular basis of interactions between proteins and membranes. Bringing together contributions from chemists, biologists and physicists, Biophysical Chemistry: Membranes and Proteins demonstrates how multidisciplinary teams can gain insights into understanding complex biological systems. This book reflects both the scope and the interdisciplinary nature of the field, with topics including: modelling of biological systems; membrane structure and interactions; probing biomolecules; and channels and receptors. Full of stimulating articles and opinions, readers from academia and industry will welcome the wide range of coverage and the state-of-the-art science.

Proteins: Concepts in Biochemistry teaches the biochemical concepts underlying protein structure, evolution, stability, folding, and enzyme kinetics, and explains how interactions in macromolecular structures determine protein function. Intended for a one-semester course in biochemistry or biophysical chemistry with a focus on proteins, this textbo

Proteins are central to all living systems and are described in this title. The first chapter describes the chemical properties of polypeptide chains and the implications of their covalent structures (Chapter 1). The conformational properties of polypeptides determine the structures that proteins can adopt (Chapter 2), to produce three-dimensional structures of incredible diversity and amazing functional properties (Chapter 3). Proteins in solution have very important dynamic properties that are crucial for their biological activities (Chapter 4). They also have a propensity to lose their folded structures and unfold, and how proteins do this and how they manageto fold to their native three-dimensional structure remains a major question (Chapter 5). The final three chapters describe the most fundamental functional properties of proteins. Central is their interactions with other molecules (Chapter 6). The most impressive and important property of proteins is their ability of catalyze the rates of chemical reactions by many orders of magnitude, and usually incredibly specifically (Chapter 7). Such potent chemical capabilities must be controlled very closely (Chapter 8).

Biophysical Characterization of Proteins in Developing Biopharmaceuticals, Second Edition, presents the latest on the analysis and characterization of the higher-order structure (HOS) or conformation of protein based drugs. Starting from the very basics of protein structure, this book explains the best way to achieve this goal using key methods commonly employed in the biopharmaceutical industry. This book will help today’s industrial scientists plan a career in this industry and successfully implement these biophysical methodologies. This updated edition has been fully revised, with new chapters focusing on the use of chromatography and electrophoresis and the biophysical characterization of very large biopharmaceuticals. In addition, best practices of applying statistical analysis to biophysical characterization data is included, along with practical issues associated with the concept of a biopharmaceutical’s developability and the technical decision-making process needed when dealing with biophysical characterization data. Presents basic protein characterization methods and tools applicable to (bio)pharmaceutical research and development Highlights the capabilities and limitations of each technique Discusses the underlining science of each tool Empowers industrial biophysical chemists by providing a roadmap for applying biophysical tools Outlines the needs for new characterization and analytical tools in the biopharmaceutical industry

DNA, RNA and proteins are undoubtedly the most important biological molecules. Being large macromolecules, their physical, chemical and biological properties can differ from those of the monomers from which they are made. This book describes their structures, origins and other key issues.

The second edition of Structure in Protein Chemistry showcases the latest developments and innovations in the field of protein structure analysis and prediction. The book begins by explaining how proteins are purified and describes methods for elucidating their sequences of amino acids and defining their posttranslational modifications. Comprehensive explanations of crystallography and of noncovalent forces-ionic interactions, hydrogen bonding, and the hydrophobic effect-act as a prelude to an exhaustive description of the atomic details of the structures of proteins. The resulting understanding of protein molecular structure forms the basis for discussions of the evolution of proteins, the symmetry of the oligomeric associations that produce them, and the chemical, mathematical, and physical basis of the techniques used to study their structures. The latter include image reconstruction, nuclear magnetic resonance spectroscopy, proton exchange, optical spectroscopy, electrophoresis, covalent cross-linking, chemical modification, immunochemistry, hydrodynamics, and the scattering of light, X-radiation, and neutrons. These procedures are applied to study the folding of polypeptides and the assembly of oligomers. Biological membranes and their proteins are also discussed. Structure in Protein Chemistry, Second Edition, bridges the gap between introductory biophysical chemistry courses and research literature. It serves as a comprehensive textbook for advanced undergraduates and graduate students in biochemistry, biophysics, and structural and molecular biology. Professionals engaged in chemical, biochemical, and molecular biological research will find it a useful reference.