Stereological techniques allow biologists to create quantitative, three-dimensional descriptions of biological structures from two- dimensional images of tissue viewed under the microscope. For example, they can accurately estimate the size of a particular organelle, the total length of a mass of capillaries, or the number of neurons or synapses in a particular region of the brain. This book provides a practical guide to designing and critically evaluating stereological studies of the nervous system and other tissues. It explains the basic concepts behind design-based stereology and how to get started. Also included are detailed descriptions of how to prepare tissue appropriately, perform pilot studies and decide on the appropriate sampling strategy, and account for phenomena such as tissue shrinkage. Numerous examples of applications of stereological methods that are applicable to studies of the central system and a wide variety of other tissues are explained. The book is therefore essential reading for neurobiologists and cell biologists interested in generating accurate representations of cell and tissue architecture.

Unbiased Stereology, Second Edition is a practical guide to making unbiased 3-D measurements via the microscope. Only those stereological techniques which have been tried and tested by real application are included. Although this technology is essentially mathematical and statistical, the authors do not immerse the reader in complex analysis, but rather provide simple heuristic explanations and references to the original proof, and illustrate the theory by analogies drawn from everyday experience. To give practical experience in application of the techniques, exercises are provided at the end of each chapter, complete with detailed worked answers.

Stereological methods provide researchers with unparalleled quantitative data from tissue samples and allow for well-evidenced research advances in a broad range of scientific fields. Presenting a concise introduction to the methodology and application of stereological research in neuroscience, Neurostereology provides a fuller understanding of the use of these methods in research and a means for replicating successful scientific approaches. Providing sound footing for future research, Neurostereology is a useful tool for basic and clinical researchers and advanced students looking to integrate these methods into their research.

"An important book for the libraries of professionals in neurobiology, neurology, and neurosurgery." -- Doody's Book Review Service

The biological sciences cover a broad array of literature types, from younger fields like molecular biology with its reliance on recent journal articles, genomic databases, and protocol manuals to classic fields such as taxonomy with its scattered literature found in monographs and journals from the past three centuries. Using the Biological Literature: A Practical Guide, Fourth Edition is an annotated guide to selected resources in the biological sciences, presenting a wide-ranging list of important sources. This completely revised edition contains numerous new resources and descriptions of all entries including textbooks. The guide emphasizes current materials in the English language and includes retrospective references for historical perspective and to provide access to the taxonomic literature. It covers both print and electronic resources including monographs, journals, databases, indexes and abstracting tools, websites, and associations—providing users with listings of authoritative informational resources of both classical and recently published works. With chapters devoted to each of the main fields in the basic biological sciences, this book offers a guide to the best and most up-to-date resources in biology. It is appropriate for anyone interested in searching the biological literature, from undergraduate students to faculty, researchers, and librarians. The guide includes a supplementary website dedicated to keeping URLs of electronic and web-based resources up to date, a popular feature continued from the third edition.

This update to Peter R. Mouton's pioneering work provides bioscientists with the concepts needed in order to apply the principles and practices of unbiased stereology to research involving biological tissues. Mouton starts with a brief explanation of the history and theory of the process before defining the terms, concepts, and tools of unbiased stereological procedures. He compares and contrasts the procedures with less-exacting approaches to quantitative analysis of biological structure using specific examples from biomedical literature. The book incorporates existing best practices with new methodologies, such as the Rare Event Protocol, while simplifying the dense, often difficult literature on the Subject to show the utility and importance of unbiased stereology. This clear, insightful guide goes a step further than other books on this Subject by demonstrating not only how to use unbiased stereology but also how to interpret and present the results. Written by the official U.S. representative to the International Society for Stereology, this is the most complete, up-to-date resource on the science of unbiased stereology. Those new to bioscience research as well as experienced practitioners will find that Mouton's explanations are the perfect companion for stereology courses and workshops.