This revised edition will continue to serve as the most complete and up-to-date guide to the use of the avian embryo in studies of vertebrate development. It will include new approaches to analysis of the chick genome, gene knock-out studies using RNA interference, morpholinos, and other cutting edge techniques. As with the original edition, emphasis has been placed on providing practical guidance, highlighting potentials and pitfalls of all key cell biological and embryological techniques. *fully revised second edition *organized into basic and advanced Methods *new section on Functional Genomics

This revised edition will continue to serve as the most complete and up-to-date guide to the use of the avian embryo in studies of vertebrate development. It will include new approaches to analysis of the chick genome, gene knock-out studies using RNA interference, morpholinos, and other cutting edge techniques. As with the original edition, emphasis has been placed on providing practical guidance, highlighting potentials and pitfalls of all key cell biological and embryological techniques. - Fully revised second edition - Organized into basic and advanced Methods - New section on Functional Genomics

This outstanding work is the only modern book devoted to the chick embryo and has been an essential resource for geneticists, molecular and developmental biologists, and other life scientists who use the chick embryo as their research model. This new enlarged and updated second edition is published in response to continuing demand. The text provides a detailed description of development, from fertilization to hatching, with emphasis on the earlier stages though also covering individual organ systems in detail. There are reviews of the more recent molecular research and a new section highlighting the important landmarks in the history of chick embryology which have had an impact on our understanding of developmental processes. The book is beautifully illustrated with 74 text-figures and over 500 photographs, including nearly 200 new scanning electron micrographs. - Updated and expanded text to accompany diagrams - More than 200 new labelled scanning electron micrographs showing individual tissues in great detail - Reviews of recent molecular research - Discusses the roles of genes such as Hox genes, BMPs, and sonic hedgehog during early development - New sections on genetical anomalies, techniques, and the poultry industry

This is the first re-appraisal in 50 years of concepts of development made in birds. This book is a case study in evolutionary diversification of life histories. Although birds have a rather uniform body plan and physiology, they exhibit marked variation in development type, parental care, and rate of growth. Altricial birds are fully dependent on their parents for warmth and nutrition and begin posthatching life in a more or less embryonic condition. At the other extreme, such superprecocial species as the megapodes are independent of all parental care from hatching, and the neonate, able to fly, resembles an adult bird. This book thus attempts to present an integrative perspective of organism biology, ecology, and evolution.

A current and authoritative guide, Methods in Avian Embryology presents a combination of classical embryological techniques and modern molecular biological approaches to studying the developing avian embryo. The only one of its kind, this book is specifically devoted to providing a detailed approach to studying avian embryos. It also describes how to use this system to study problems in cell, developmental, and neurobiology. The protocols emphasize microsurgery, histology, and cellular and molecular marking, which are not covered in the usual molecular biology methods manuals. The methods include: embryonic transplantations, cell culture and organ culture, in situ hybridization, classical histological techniques, and retrovirally mediated gene transfer. Key Features* Complete and easy-to-follow procedures* Helpful illustrations* Distinguished group of authors* Wide range of approaches

The second edition of Avian Immunology provides an up-to-date overview of the current knowledge of avian immunology. From the ontogeny of the avian immune system to practical application in vaccinology, the book encompasses all aspects of innate and adaptive immunity in chickens. In addition, chapters are devoted to the immunology of other commercially important species such as turkeys and ducks, and to ecoimmunology summarizing the knowledge of immune responses in free-living birds often in relation to reproductive success. The book contains a detailed description of the avian innate immune system, encompassing the mucosal, enteric, respiratory and reproductive systems. The diseases and disorders it covers include immunodepressive diseases and immune evasion, autoimmune diseases, and tumors of the immune system. Practical aspects of vaccination are examined as well. Extensive appendices summarize resources for scientists including cell lines, inbred chicken lines, cytokines, chemokines, and monoclonal antibodies. The world-wide importance of poultry protein for the human diet, as well as the threat of avian influenza pandemics like H5N1 and heavy reliance on vaccination to protect commercial flocks makes this book a vital resource. This book provides crucial information not only for poultry health professionals and avian biologists, but also for comparative and veterinary immunologists, graduate students and veterinary students with an interest in avian immunology. - With contributions from 33 of the foremost international experts in the field, this book provides the most up-to-date review of avian immunology so far - Contains a detailed description of the avian innate immune system reviewing constitutive barriers, chemical and cellular responses; it includes a comprehensive review of avian Toll-like receptors - Contains a wide-ranging review of the "ecoimmunology" of free-living avian species, as applied to studies of population dynamics, and reviews methods and resources available for carrying out such research

This book reviews comprehensively incubation effects on embryonic development in birds and reptiles and presents the first ever synthesis of data from these two vertebrate classes. The book is in three parts. The first deals with the structure, shape and function of eggs. The second examines the effects of the four main parameters on the process of incubation: temperature, water relations, respiratory gas exchange, and turning. The third section deals with early embryonic development and the methods used to investigate and manipulate the embryo. Further chapters deal with aestivation, megapodes and oviparity. International experts in each field have contributed to this extensively referenced volume and it will be of great interest not only to research biologists, but also to bird and reptile breeders, whether in commercial organisations or in zoos.