This book is a thought-provoking assessment of assumptions inhibiting progress in comparative biology. The volume is inspired by a list generated years earlier by Donn Rosen, one of the most influential, innovative and productive comparative biologists of the latter 20th century. His list has assumed almost legendary status among comparative evolutionary biologists. Surprisingly many of the obstructing assumptions implicated by Rosen remain relevant today. Any comparative biologist hoping to avoid such assumptions in their own research will benefit from this introspective volume.

This Open Access volume provides comprehensive reviews and describes the latest techniques to study eukaryotic ribosome biogenesis. For more than 50 years ribosomes are a major research topic. Our knowledge about ribosome biogenesis and function such as transcription, mRNA modification, and translation was the sine qua non for developing the powerful RNA-based vaccines against RNA-viruses causing the world-threatening Covid-19 pandemia. The chapters in this book are organized into six parts. Part One discusses a comparative survey about the unity and diversity of ribosome biogenesis in pro- and eukaryotic cells. Part Two deals with the genomic organization of eukaryotic rDNA and the role of RNA polymerase I in ribosomal RNA transcription. Part Three explores in vitro methods to study RNA polymerase I structure and its function, and Part Four analyzes the nucleo-cytoplasmic transport of assembled ribosomes and RNP complexes. Part Five covers modifications that increase the complexity of rRNAs, and Part Six provides readers with a review of eukaryotic translation and - for the first time - describes a new method to analyze translation in vitro. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Ribosome Biogenesis: Methods and Protocols is a valuable resource for scientists and researchers interested in learning more about the increasing importance of in vitro RNA-technologies.

This book includes all 14 articles contributed to the Special Issue "Systematics and Conservation of Neotropical Amphibians and Reptiles” in the journal Diversity, originally published in 2019 and 2020.

Thousands of religions have adherents today, and countless more have existed throughout history. What accounts for this astonishing diversity? This extraordinarily ambitious and comprehensive book demonstrates how evolutionary systematics and philosophy can yield new insight into the development of organized religion. Lance Grande—a leading evolutionary systematist—examines the growth and diversification of hundreds of religions over time, highlighting their historical interrelationships. Combining evolutionary theory with a wealth of cultural records, he explores the formation, extinction, and diversification of different world religions, including the many branches of Asian cyclicism, polytheism, and monotheism. Grande deploys an illuminating graphic system of evolutionary trees to illustrate historical interrelationships among the world’s major religious traditions, rejecting colonialist and hierarchical “ladder of progress” views of evolution. Extensive and informative illustrations clearly and vividly indicate complex historical developments and help readers grasp the breadth of interconnections across eras and cultures. The Evolution of Religions marshals compelling evidence, starting far back in time, that all major belief systems are related, despite the many conflicts that have taken place among them. By emphasizing these broad historical interconnections, this book promotes the need for greater tolerance and deeper, unbiased understanding of cultural diversity. Such traits may be necessary for the future survival of humanity.

was the result of the efforts of Robert Cleverdon. The rapidly developing discipline of molecular biology and the rapidly expanding knowledge of the PPLO were brought together at this meeting. In addition to the PPLO specialists, the conference invited Julius Marmur to compare PPLO DNA to DNA of other organisms; David Garfinkel, who was one of the first to develop computer models of metabolism; Cyrus Levinthal to talk about coding; and Henry Quastler to discuss information theory constraints on very small cells. The conference was an announcement of the role of PPLO in the fundamental understanding of molecular biology. Looking back 40-some years to the Connecticut meeting, it was a rather bold enterprise. The meeting was international and inter-disciplinary and began a series of important collaborations with influences resonating down to the present. If I may be allowed a personal remark, it was where I first met Shmuel Razin, who has been a leading figure in the emerging mycoplasma research and a good friend. This present volume is in some ways the fulfillment of the promise of that early meeting. It is an example of the collaborative work of scientists in building an understanding of fundamental aspects of biology.