Explores the principles, and presents step-by-step recipes for isolating and measuring biological materials using modern immunochemical methods, including such new procedures as high-performance immunoaffinity chromatography, capillary electrophoresis, and blotting. Also includes clear drawings of

Analytical Techniques in Biosciences: From Basics to Applications presents comprehensive and up-to-date information on the various analytical techniques obtainable in bioscience research laboratories across the world. This book contains chapters that discuss the basic bioanalytical protocols and sample preparation guidelines. Commonly encountered analytical techniques, their working principles, and applications were presented. Techniques, considered in this book, include centrifugation techniques, electrophoretic techniques, chromatography, titrimetry, spectrometry, and hyphenated techniques. Subsequent chapters emphasize molecular weight determination and electroanalytical techniques, biosensors, and enzyme assay protocols. Other chapters detail microbial techniques, statistical methods, computational modeling, and immunology and immunochemistry.The book draws from experts from key institutions around the globe, who have simplified the chapters in a way that will be useful to early-stage researchers as well as advanced scientists. It is also carefully structured and integrated sequentially to aid flow, consistency, and continuity. This is a must-have reference for graduate students and researchers in the field of biosciences. - Presents basic analytical protocols and sample-preparation guidelines - Details the various analytical techniques, including centrifugation, spectrometry, chromatography, and titrimetry - Describes advanced techniques such as hyphenated techniques, electroanalytical techniques, and the application of biosensors in biomedical research - Presents biostatistical tools and methods and basic computational models in biosciences

The book appeared in two previous Slovak editions for university students in Czechoslovakia. This edition presents a completely new version updated according to recent advances not only in immunochemistry and essential immunology but also in molecular biology, biochemistry and molecular genetics. The scope of the book is considerable since the goal was to cover the field of immunochemistry from the widest point of view including both the topic and methods of contemporary immunochemistry. Each chapter provides basic information on a specific subtopic, clearly and understandab ly, and presents principles of individual immunochemical methods. I am confident that the book will fill the gap between the books on essential immunology and highly specialised books on individual areas of immunoche mistry (e. g. on antibodies, antigens, numerous immunochemical techniques, etc. ). It may also prove useful for beginning investigators from different biological and medical fields as it supplies basic information needed for solving their scientific problems by immunochemical approaches. I do hope that readers will find the text stimulatory and pleasury to read. I wish to thank all colleagues and friends for supplying their own results, suggestions and for their encouraging comments. My thanks go also to the editors and publishers for their valuable contribution to the preparation of the book. 1 Introduction The term immunochemistry was coined by the Swedish chemist ARRHENIUS who used it for the first time in his lectures in 1907.

This concise yet comprehensive guide to the methods and protocols of immunohistochemistry covers established techniques and current developments in the field such as the use of epitope tags, multiple immunolabeling and diagnostic immunohistochemistry.

Immunochemistry of Solid-Phase Immunoassay fills a niché in the field of immunoassay and immunology. Although solid-phase immunoassay constitutes a major technology in biology and medicine, there is no comprehensive source devoted to the immunochemical principles involved. As a result, this book will benefit students, technicians, and researchers who use this technology, as well as immunodiagnostic and biotech companies who develop the technology. The book is not a methods manual; instead, it incorporates the concepts, data, and opinions of more than 25 investigators working in this field. Topics discussed include: the chemistry of solid-phases, the behavior or antibodies and antigens on solid phases, membrane solid-phases, reaction kinetics, antigen quantitation, enzyme systems, photophysics, immunochemical considerations in data analyses, multianalyte assays and occupancy concepts, antibody quantitation, streptavidin, a review of data analysis software, and solid-phase peptide immunoassay.

This volume provides a comprehensive reference guide for researchers to study the applications of labeled antibodies. Chapters guide reader through the the and practice of immunohistochemistry, immunocytochemistry and immunofluorescence techniques. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and useful tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Immunohistochemistry and Immunofluorescence: Methods and Protocols aims to be a useful practical guide to scientists to help further their study in this field.