Presents a fully updated, self-contained textbook covering the core theory and practice of both classical and modern optical microscopy techniques.

This book offers a beginner’s guide to using light microscopes. It begins with a brief introduction to the physics of optics, which will give the reader a basic grasp of the behaviors of light. In turn, each part of the microscope is explained using clear and simple English, together withdetailed photographs and diagrams. The reader will learn the function, care and correct use of each part. A troubleshooting section also helps resolve some of the most common issues encountered in light microscopy. Most people have a general idea of how to use a microscope, but many never get the full benefit, because they receive no training. With easy-to-follow steps and detailed images, this guide will help everyone achieve the best results, and be confident using their microscope. This book is intended for anyone using a light microscope, such as university students, people in lab environments, hobbyists, educators who teach science to young children, and anyone with a general interest in these valuable tools.

This book provides a comprehensive introduction to the field of scanning optical microscopy for scientists and engineers. The book concentrates mainly on two instruments: the Confocal Scanning Optical Microscope (CSOM), and the Optical Interference Microscope (OIM). A comprehensive discussion of the theory and design of the Near-Field Scanning Optical Microscope (NSOM) is also given. The text discusses the practical aspects of building a confocal scanning optical microscope or optical interference microscope, and the applications of these microscopes to phase imaging, biological imaging, and semiconductor inspection and metrology.A comprehensive theoretical discussion of the depth and transverse resolution is given with emphasis placed on the practical results of the theoretical calculations and how these can be used to help understand the operation of these microscopes. - Provides a comprehensive introduction to the field of scanning optical microscopy for scientists and engineers - Explains many practical applications of scanning optical and interference microscopy in such diverse fields as biology and semiconductor metrology - Discusses in theoretical terms the origin of the improved depth and transverse resolution of scanning optical and interference microscopes with emphasis on the practical results of the theoretical calculations - Considers the practical aspects of building a confocal scanning or interference microscope and explores some of the design tradeoffs made for microscopes used in various applications - Discusses the theory and design of near-field optical microscopes - Explains phase imaging in the scanning optical and interference microscopes

Optical microscopy is one of the most valuable--but under utilized--tools for analyzing fiber reinforced polymer matrix composites. This hands-on instructional book covers everything: sample preparation, microscopic techniques, and applications. The power of optical microscopy to study the microstructure of these heterogeneous, anisotropic materials is illustrated with over 180 full color images.

Choice Recommended Title, March 2020 Optical microscopy is used in a vast range of applications ranging from materials engineering to in vivo observations and clinical diagnosis, and thanks to the latest advances in technology, there has been a rapid growth in the number of methods available. This book is aimed at providing users with a practical guide to help them select, and then use, the most suitable method for their application. It explores the principles behind the different forms of optical microscopy, without the use of complex maths, to provide an understanding to help the reader utilise a specific method and then interpret the results. Detailed physics is provided in boxed sections, which can be bypassed by the non-specialist. It is an invaluable tool for use within research groups and laboratories in the life and physical sciences, acting as a first source for practical information to guide less experienced users (or those new to a particular methodology) on the range of techniques available. Features: The first book to cover all current optical microscopy methods for practical applications Written to be understood by a non-optical expert with inserts to provide the physical science background Brings together conventional widefield and confocal microscopy, with advanced non-linear and super resolution methods, in one book To learn more about the author please visit here.

Knowledge of microscope design is rapidly becoming more important. Microscopes are used in critical applications such as drug development, clinical tests, and genomics. Considerable expertise is required for the evaluation, design, and manufacture of these instruments. Several subsystems must be integrated: the source, the illumination optics, the specimen, the objective lens, the tube optics, and the sensor. The large numerical aperture of a microscope is essential for small spot size and high brightness; however, the large numerical aperture also presents difficult issues in optical design and fabrication. This book provides a foundation for developing design expertise through education, practice, and exploration. It is suitable for lens designers, optical engineers, and students with a basic knowledge of microscope structure.