Biophotonics involves understanding how light interacts with biological matter, from molecules and cells, to tissues and even whole organisms. Light can be used to probe biomolecular events, such as gene expression and protein-protein interaction, with impressively high sensitivity and specificity. The spatial and temporal distribution of biochemic

Despite a number of books on biophotonics imaging for medical diagnostics and therapy, the field still lacks a comprehensive imaging book that describes state-of-the-art biophotonics imaging approaches intensively developed in recent years. Addressing this shortfall, Advanced Biophotonics: Tissue Optical Sectioning presents contemporary methods and

The field of biophotonics is rapidly emerging in both academia and industry. It is the convergence of photonics and life sciences. Photonics - the science and technology of light generation, manipulation and measurement - has itself seen a remarkable expansion in the past 20 years, both in research and in commercialization, particularly in telecommunications. The life sciences have an increasing need for new technologies to which photonics can make significant contributions. As biology and medicine move into the post-genomics era, it is increasingly important to have highly sensitive tools for probing cells, tissues and whole organism structure and functions. Through photonic technologies optical fibers and sensitive imaging detectors, these measurements can often be done in a non- or minimally-invasive way, which is tremendously valuable for clinical and remote-sensing applications. In clinical medicine the ability to probe and image tissues is leading to a wide range of novel diagnostic methods; examples of these techniques are given in this book. Finally, the new field of nanotechnology is now penetrating into biophotonics. Examples include the use of nanoparticles such as metal nanospheres or rods and quantum dots for enhanced cell and tissue imaging and local light energy absorption. As will be evident, this volume is not intended as a comprehensive text on biophotonics. Rather, it presents ‘snapshots’ of some of the most exciting developments, from a perspective of photonic technologies, and life-sciences applications.

Paras Prasad’s text provides a basic knowledge of a broad range of topics so that individuals in all disciplines can rapidly acquire the minimal necessary background for research and development in biophotonics. Introduction to Biophotonics serves as both a textbook for education and training as well as a reference book that aids research and development of those areas integrating light, photonics, and biological systems. Each chapter contains a topic introduction, a review of key data, and description of future directions for technical innovation. Introduction to Biophotonics covers the basic principles of Optics Optical spectroscopy Microscopy Each section also includes illustrated examples and review questions to test and advance the reader’s knowledge. Sections on biosensors and chemosensors, important tools for combating biological and chemical terrorism, will be of particular interest to professionals in toxicology and other environmental disciplines. Introduction to Biophotonics proves a valuable reference for graduate students and researchers in engineering, chemistry, and the life sciences.

Presents recent developments and application of fluorescent protein-labelling techniques and two-photon molecular probes. Introduces the theoretical and experimental researches of super-resolution localization microscopy, photoacoustic molecular (functional) imaging, and optical molecular tomography for small animal in vivo. Illustrates optical labeling techniques and imaging instruments and their application in biological studies. Suits well for researchers and graduates in biomolecular photonics fields.

Biophotonics is a burgeoning field that has afforded researchers and medical practitioners alike an invaluable tool for implementing optical microscopy. Recent advances in research have enabled scientists to measure and visualize the structural composition of cells and tissue while generating applications that aid in the detection of diseases such as cancer, Alzheimer’s, and atherosclerosis. Rather than divulge a perfunctory glance into the field of biophotonics, this textbook aims to fully immerse senior undergraduates, graduates, and research professionals in the fundamental knowledge necessary for acquiring a more advanced awareness of concepts and pushing the field beyond its current boundaries. The authors furnish readers with a pragmatic, quantitative, and systematic view of biophotonics, engaging such topics as light-tissue interaction, the use of optical instrumentation, and formulating new methods for performing analysis. Designed for use in classroom lectures, seminars, or professional laboratories, the inclusion and incorporation of this textbook can greatly benefit readers as it serves as a comprehensive introduction to current optical techniques used in biomedical applications. Caters to the needs of graduate and undergraduate students as well as R&D professionals engaged in biophotonics research. Guides readers in the field of biophotonics, beginning with basic concepts before proceeding to more advanced topics and applications. Serves as a primary text for attaining an in-depth, systematic view of principles and applications related to biophotonics. Presents a quantitative overview of the fundamentals of biophotonic technologies. Equips readers to apply fundamentals to practical aspects of biophotonics.

This book introduces senior-level and postgraduate students to the principles and applications of biophotonics. It also serves as a valuable reference resource or as a short-course textbook for practicing physicians, clinicians, biomedical researchers, healthcare professionals, and biomedical engineers and technicians dealing with the design, development, and application of photonics components and instrumentation to biophotonics issues. The topics include the fundamentals of optics and photonics, the optical properties of biological tissues, light-tissue interactions, microscopy for visualizing tissue components, spectroscopy for optically analyzing the properties of tissue, and optical biomedical imaging. It also describes tools and techniques such as laser and LED optical sources, photodetectors, optical fibers, bioluminescent probes for labeling cells, optical-based biosensors, surface plasmon resonance, and lab-on-a-chip technologies. Among the applications are optical coherence tomography (OCT), optical imaging modalities, photodynamic therapy (PDT), photobiostimulation or low-level light therapy (LLLT), diverse microscopic and spectroscopic techniques, tissue characterization, laser tissue ablation, optical trapping, and optogenetics. Worked examples further explain the material and how it can be applied to practical designs, and the homework problems help test readers’ understanding of the text.