Advanced Photon Counting
Title | Advanced Photon Counting PDF eBook |
Author | Peter Kapusta |
Publisher | Springer |
Pages | 371 |
Release | 2015-04-23 |
Genre | Science |
ISBN | 3319156365 |
This volume focuses on Time-Correlated Single Photon Counting (TCSPC), a powerful tool allowing luminescence lifetime measurements to be made with high temporal resolution, even on single molecules. Combining spectrum and lifetime provides a “fingerprint” for identifying such molecules in the presence of a background. Used together with confocal detection, this permits single-molecule spectroscopy and microscopy in addition to ensemble measurements, opening up an enormous range of hot life science applications such as fluorescence lifetime imaging (FLIM) and measurement of Förster Resonant Energy Transfer (FRET) for the investigation of protein folding and interaction. Several technology-related chapters present both the basics and current state-of-the-art, in particular of TCSPC electronics, photon detectors and lasers. The remaining chapters cover a broad range of applications and methodologies for experiments and data analysis, including the life sciences, defect centers in diamonds, super-resolution microscopy, and optical tomography. The chapters detailing new options arising from the combination of classic TCSPC and fluorescence lifetime with methods based on intensity fluctuation represent a particularly unique highlight.
Advanced Photon Counting
Title | Advanced Photon Counting PDF eBook |
Author | Peter Kapusta |
Publisher | |
Pages | |
Release | 2015 |
Genre | |
ISBN | 9783319156378 |
This volume focuses on Time-Correlated Single Photon Counting (TCSPC), a powerful tool allowing luminescence lifetime measurements to be made with high temporal resolution, even on single molecules. Combining spectrum and lifetime provides a "fingerprint" for identifying such molecules in the presence of a background. Used together with confocal detection, this permits single-molecule spectroscopy and microscopy in addition to ensemble measurements, opening up an enormous range of hot life science applications such as fluorescence lifetime imaging (FLIM) and measurement of Förster Resonant Energy Transfer (FRET) for the investigation of protein folding and interaction. Several technology-related chapters present both the basics and current state-of-the-art, in particular of TCSPC electronics, photon detectors and lasers. The remaining chapters cover a broad range of applications and methodologies for experiments and data analysis, including the life sciences, defect centers in diamonds, super-resolution microscopy, and optical tomography. The chapters detailing new options arising from the combination of classic TCSPC and fluorescence lifetime with methods based on intensity fluctuation represent a particularly unique highlight.
Advanced Photon Counting Techniques VIII.
Title | Advanced Photon Counting Techniques VIII. PDF eBook |
Author | |
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Pages | |
Release | 2014 |
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Advanced Photon Counting Techniques X.
Title | Advanced Photon Counting Techniques X. PDF eBook |
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Advanced Photon Counting Techniques VI
Title | Advanced Photon Counting Techniques VI PDF eBook |
Author | Mark A. Itzler |
Publisher | |
Pages | 250 |
Release | |
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ISBN |
Annotation Includes Proceedings Vol. 7821.
Advanced Photon Counting Techniques XII
Title | Advanced Photon Counting Techniques XII PDF eBook |
Author | |
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Pages | |
Release | 2018 |
Genre | |
ISBN | 9781510618299 |
Advanced Photon Counting Techniques VI.
Title | Advanced Photon Counting Techniques VI. PDF eBook |
Author | |
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Release | 2012 |
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