Adipose Tissue Protocols

Adipose Tissue Protocols
Title Adipose Tissue Protocols PDF eBook
Author Kaiping Yang
Publisher Humana Press
Pages 336
Release 2010-10-28
Genre Science
ISBN 9781617378447

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The radical expansion and revision of techniques and experimental approaches employed in the study of adipose tissue are reflected in this revised book. Several updated chapters are combined with many new contributions from internationally recognized experts. Conventional and state-of-the-art methodologies are covered, as well as whole animal, cellular and molecular approaches which have emerged in or been extended to adipose tissue research over the past few years. This revised all-in-one manual is essential reading for newcomers to this burgeoning area as well as a valuable volume for more experienced researchers.

Adipose Tissue Protocols

Adipose Tissue Protocols
Title Adipose Tissue Protocols PDF eBook
Author Gérard Ailhaud
Publisher Springer Science & Business Media
Pages 332
Release 2008-02-03
Genre Science
ISBN 1592592317

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Adipose tissue is recognized to be exquisitely sensitive to hormone action, and is also now recognized as a secretory and endocrine organ required for reproduction and good health. Adipocytes are “smart” cells able within the tissue to communicate with surrounding cells, but also with various organs, particularly via leptin acting on the central nervous system. Brown adipose tissue (BAT) and white adipose tissue (WAT) are known to be distinct tissues, whereas the heterogeneity of WAT depots is well est- lished. Unfortunately, excess WAT leads to obesity, which is the most common health problem in industrialized countries. Therefore, from both a scientific and a technical point of view, the time has come to create a survey of adipose tissues and their neglected adipocytes. In Adipose Tissue Protocols, I have attempted to gather together chapters from all areas of adipose tissue research—from in vivo to in vitro studies—and to provide methods covering a wide variety of techniques, including the choice of adipose tissue depot and of morphological techniques for the study of BAT and WAT; the isolation, subcellular fractionation, and transfection of adipocytes where the low density of these cells must be taken into account; assays of nutrient and ion fluxes and the metabolic aspects of nutrient uptake; assays of lipid-related enzymes; biopsies and quantification of lipid-related mRNAs; cultures of adipose precursor cells from WAT and BAT of various species, including human tissue; measurements of adipose secretory products; and assessment of WAT metabolism in vivo.

Adipose-Derived Stem Cells

Adipose-Derived Stem Cells
Title Adipose-Derived Stem Cells PDF eBook
Author Jeffrey M. Gimble
Publisher Humana Press
Pages 474
Release 2011-08-24
Genre Science
ISBN 9781617379611

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During the past decade, a wide range of scientific disciplines have adopted the use of adipose-derived stem/stromal cells (ASCs) as an important tool for research and discovery. In Adipose-Derived Stem Cells: Methods and Protocols, experts from the field, including members of the esteemed International Federation of Adipose Therapeutics and Science (IFATS), provide defined and established protocols in order to further codify the utilization of these powerful and accessible cells. With chapters organized around approaches spanning the discovery, pre-clinical, and clinical processes, much of the emphasis is placed on human ASC, while additional techniques involving small and large animal species are included. As a volume in the highly successful Methods in Molecular BiologyTM series, the detailed contributions include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Adipose-Derived Stem Cells: Methods and Protocols serves as a vital reference text for experienced researchers as well as new students on the path to further exploring the incredible potential of ASCs.

Monitoring Human Tissues for Toxic Substances

Monitoring Human Tissues for Toxic Substances
Title Monitoring Human Tissues for Toxic Substances PDF eBook
Author National Research Council
Publisher National Academies Press
Pages 224
Release 1991-02-01
Genre Science
ISBN 0309044375

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The National Human Monitoring Program (NHMP) identifies concentrations of specific chemicals in human tissues, including toxicologic testing and risk assessment determinations. This volume evaluates the current activities of the NHMP; identifies important scientific, technical, and programmatic issues; and makes recommendations regarding the design of the program and use of its products.

Skin Tissue Engineering

Skin Tissue Engineering
Title Skin Tissue Engineering PDF eBook
Author Sophie Böttcher-Haberzeth
Publisher Springer Nature
Pages 260
Release 2019-05-30
Genre Science
ISBN 1493994735

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This volume provides protocols describing the isolation and culture of diverse cell types stemming from the skin and the use of these cells and cell constructs for wound healing, bioengineering applications, and translational medicine purposes. The book is divided into three sections describing the isolation and culture of diverse skin cells, managing these cells within co-culture systems and skin models, as well as using these skin models in a test setting. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Skin Tissue Engineering: Methods and Protocols serves as a vital aid to basic and clinical researchers such as biologists, physicians, and biomedical engineers working with and being interested in basic science, and clinically and laboratory-applicable translational regenerative medicine.

Angiogenesis in Adipose Tissue

Angiogenesis in Adipose Tissue
Title Angiogenesis in Adipose Tissue PDF eBook
Author Yihai Cao
Publisher Springer Science & Business Media
Pages 374
Release 2013-09-05
Genre Medical
ISBN 1461480698

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Angiogenesis has recently played a critical role in regulation of adipose tissue expansion and regression. Like most other tissues in the body, adipose expansion and regression is accompanied by alteration of blood vessel density and structures. The vascular alteration plays an active role in regulation of adipose tissue size and functions. Targeting blood vessels in the adipose tissue have demonstrated to be a novel approach for possibly treatment of cancer, obesity and other metabolic diseases. This book provides the most updated information on this type research and discusses future opportunities for therapy..

The Adipose Organ

The Adipose Organ
Title The Adipose Organ PDF eBook
Author Saverio Cinti
Publisher
Pages 94
Release 1999
Genre Medical
ISBN 9788885030329

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