A Textbook of Pharmacology II (according to PCI regulation) is the title of the book. The writers' original intention for the book was to provide a consolidated database enabling a simple comprehension of drug pharmacology. This book aims to enlighten readers on cutting-edge drug delivery methods and provide guidance to tutors and students on the essential ideas of Pharmacology II. The primary goal in writing this textbook was to fulfill undergraduates' needs in accordance with PCI regulations by presenting material in an eloquent, concise manner. This volume is intended to inform postgraduate students on Pharmaceutical Jurisprudence in addition to adhering to the PCI curriculum for pharmacy undergraduate degrees. We guarantee that this book will be highly appreciated by academics, professionals in the field, postgraduate students, and graduates. Nonetheless, recommendations for how to make the text better are welcome and will be duly noted.

The major aim to write this textbook is to provide information in articulate summarized manner to accomplish necessities of undergraduates as per PCI regulation. This volume is designed not only according to curriculum of undergraduate courses in pharmacy by PCI but also to communicate knowledge on Pharmaceutical Jurisprudence for post graduate learners. We assured this book will be originated very valuable by graduates, post graduates, professors and industrial learners. However any suggestion for further improvement of text are welcome and will be taken due note of.

It gives immense pleasure to introduce the book, “Pharmacology II”. The Book has been strictly written as per the syllabus prescribed by the Pharmacy Council of India, for Bachelor in Pharmacy students. Pharmacology and therapeutics is the base for the disease management. This book comprising all pharmacological and clinical treatments schemes and drug combinations for various diseases and also contains pathophysiological with treatment targets. In this book, major focused on pharmacology of star drug of respective category includes contraindications, indications and ADR, Drug Interaction and pharmacokinetics in a very simple and understandable manner.

1 From Classical Genetics to Modern Genetics 2 Genetic Engineering and r-DNA Technology 3 Genetic Complexity 4 Enzymes used in Gene Manipulation of DNA and RNA 5 Restriction Endonucleases-Molecular Scalpels 6 Isolation and Purification of DNA 7 Plasmids 8 Isolation and Purification of Plasmid DNA 9 Preparation of RNA 10 Autoradiography 11 Electrophoresis of DNA 12 Pulsed Field Gel Electrophoresis (PFGE) 13 Nucleic Acid Staining 14 Nucleic Acid Labelling 15 Molecular Probes 16 Hybridization Techniques 17 DNA Fingerprinting (DNA Profiling) 18 Restriction Fragment Length Polymorphism (RFLP) 19 Slotting Techniques 20 Vectors 21 Gene Cloning 22 Screening of Recombinants 23 Protoplast Technology - Fusion Techniques 24 DNA-Protein Interactions 25 DNA Libraries - (a) Genomic Library (b) cDNA Library 26 Chromosome Walking and Jumping 27 Site-Directed Mutagenesis 28 DNA Synthesis 29 Techniques of (Chromosome) Gene Mapping 30 DNA Sequencing (Gene Sequencing) 31 Ribozymes 32 Polymerase Chain Reaction (PCR) 33 Random Amplification of Polymorphic DNA (RAPD) 34 Subtraction Hybridization 35 Ribotyping 36 Protein Engineering 37 Strain Imr.;rovement of Industrially Important Organisms 38 Gene Transfer Technologies (DNA Transfer Methods) 39 Transgenic Animals 40 Human Gene Therapy 41 Gene Targeting 42 Antisense Therapy 43 RNA Interference 44 Flow Cytogenetics 45 Human Genome Project - Part I 45 Human Genome Project - Part II 47 Genomics 48 Proteomics 49 Bioinformatics - I (Primer) 50 Bioinformatics - II (Tools and Techniques) 51 Ethical, Legal, Social, Environmental and Health Issues Related to Gene Biotechnology 52 Sythetic Biology Glossary References.

1 General Anaesthetics 2 Local anaesthetics 3 Alcohols And alcoholism 4 Antipsychotics 5 Anti anxiety Drugs 6 Sedatives and Hydnotics 7 Antidepressant drugs 8 Antimanic drugs Mood stabilising agents 9 CNS stimulants 10 Antiepileptic drugs 11 Pharmacotherapy of parkinsons disease 12 Pharmacotherapy of alzheimers disease 13 Opioid analgesics and antagonists 14 Non steroidal anti inflammatory agents 15 Pharmacotherapy of rheumatoid arthritis 16 Pharmacotherapy of gout 17 Drugs used in bronchial asthma and COPD 18 Nasal decongestants expectorants and antitussive agents 19 Pharmacotherapy of peptic ulcer 20 Pharmacology of emetics and anti emetics 21 pharmacotherapy of contipation 22 pharmacotherapy of diarrhoea 23 Question Bank of pune university B Pharm Pharmacology Examination

About the Author : - SD Seth is currently Chair in Clinical Pharmacology at the ICMR and an honorary Advisor to the Clinical Trials Registry India. He has served as a faculty in AIIMS for 29 years. He is the founder member of the National Poisons Information Centre at AIIMS. Professor Seth is a member of several prestigious Committees like the Scientific Advisory Committee of the Drugs for Neglected Diseases Initiative, Geneva, Drugs Technical Advisory Board, Investigational New Drug Committee, National Pharmacovigilance Steering Committee, and other committees of ICMR, CSIR, DST, DBT and Ministry of Health.Vimlesh Seth has a teaching experience of 30 years at the Department of Paediatrics, AIIMS. She has been a recipient of the award James Flett Gold Medal for her work in growth and development of children. In addition, research work guided by her has been awarded the President's medal for the Indian Rheumatic Association, Dr Vaishnav Award and PV Sukhatam Award.

Introduction 2. Synthesis Of Some Official Medicinal Compounds 3. Assay Of Some Official Compounds 4. Monograph Analysis Of The Following Compounds 5. Identification And Estimation Of Drug Metabolites From Biological Fluids 6. Determination Of Partition Coefficient Of Compounds For Qsar Analysis 7. I.R. Spectra Of Some Official Medicinal Compounds