A Complete Guide to Gene Cloning: From Basic to Advanced

A Complete Guide to Gene Cloning: From Basic to Advanced
Title A Complete Guide to Gene Cloning: From Basic to Advanced PDF eBook
Author Nayana Patil
Publisher Springer Nature
Pages 177
Release 2022-04-26
Genre Science
ISBN 3030968510

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This comprehensive guide to gene cloning provides beginning and advanced readers with the background, standard techniques, practical applications, and ethical and safety considerations in the field. A one-stop reference for students, researchers in academia and industry, and anyone interested in a thorough but accessible overview.

Molecular Biology of the Cell

Molecular Biology of the Cell
Title Molecular Biology of the Cell PDF eBook
Author
Publisher
Pages 0
Release 2002
Genre Cells
ISBN 9780815332183

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Molecular Biology Techniques

Molecular Biology Techniques
Title Molecular Biology Techniques PDF eBook
Author Heather B. Miller
Publisher Academic Press
Pages 227
Release 2011-10-18
Genre Science
ISBN 0123855454

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This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein. The third edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The "project approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein - students can actually visualize positive clones following IPTG induction. - Cover basic concepts and techniques used in molecular biology research labs - Student-tested labs proven successful in a real classroom laboratories - Exercises simulate a cloning project that would be performed in a real research lab - "Project" approach to experiments gives students an overview of the entire process - Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions

Gene Cloning and Manipulation

Gene Cloning and Manipulation
Title Gene Cloning and Manipulation PDF eBook
Author Christopher Howe
Publisher Cambridge University Press
Pages 250
Release 2007-07-12
Genre Medical
ISBN 1139463586

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Updated to reflect advances in the field, this introduction provides a broad, but concise, coverage of recombinant DNA techniques. Written for advanced undergraduates, graduates and scientists who want to use this technology, emphasis is placed on the concepts underlying particular types of cloning vectors to aid understanding and to enable readers to devise suitable strategies for novel experimental situations. An introduction to the basic biochemical principles is presented first. Then PCR and cloning using E. coli hosts and plasmid, phage and hybrid vectors are described, followed by the generation and screening of libraries and how to modify, inactivate or express cloned sequences. Finally genetic manipulation in a range of other organisms is discussed, including other bacteria, fungi, algae and plants, insects and mammals. A series of 'real-life' biological problems are also presented to enable readers to assess their understanding of the material and to prepare for exams.

Synthetic Biology for Therapeutics

Synthetic Biology for Therapeutics
Title Synthetic Biology for Therapeutics PDF eBook
Author Urartu Ozgur Safak Seker
Publisher Walter de Gruyter GmbH & Co KG
Pages 184
Release 2024-11-18
Genre Science
ISBN 3111329496

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Synthetic biology is enabling scientists to use engineered genetic circuits in the cells as the basis for the development of new living therapeutics and as a powerful new weapon in the fight against diseases, especially cancer. Bacteria-mediated therapy is a promising alternative cancer treatment. The book covers the recent developments of cellular therapies from a synthetic biology perspective including engineered microbial therapies, CAR-T therapies etc. and is an indispensable guide to scientists in both biotechnology and medicine.

A Practical Guide to Protein Engineering

A Practical Guide to Protein Engineering
Title A Practical Guide to Protein Engineering PDF eBook
Author Tuck Seng Wong
Publisher Springer Nature
Pages 213
Release 2020-10-29
Genre Science
ISBN 3030568989

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This textbook introduces readers in an accessible and engaging way to the nuts and bolts of protein expression and engineering. Various case studies illustrate each step from the early sequence searches in online databases over plasmid design and molecular cloning techniques to protein purification and characterization. Furthermore, readers are provided with practical tips to successfully pursue a career as a protein engineer. With protein engineering being a fundamental technique in almost all molecular biology labs, the book targets advanced undergraduates and graduate students working in molecular biology, biotechnology and related scientific fields.

Technologies in Cell Culture

Technologies in Cell Culture
Title Technologies in Cell Culture PDF eBook
Author
Publisher BoD – Books on Demand
Pages 296
Release 2024-06-26
Genre Science
ISBN 1837682917

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Technologies in Cell Culture - A Journey From Basics to Advanced Applications is a comprehensive book that offers a broad overview of the subject, encompassing fundamental concepts, modern techniques, and their diverse applications across various fields. Comprising eleven chapters authored by leading international experts in their respective fields, this book adeptly navigates the complexities of cell culture. It provides valuable insights into bioprocessing, cancer biology, regenerative medicine, and more. The book explores innovative strategies for restoring eyesight in individuals with age-related macular degeneration through retinal pigment epithelium monolayers derived from autologous adipose tissue stem cells. We discover novel approaches for utilizing in vitro techniques to evaluate new medications targeting crucial molecular pathways. A deeper comprehension of the tumor microenvironment can be achieved by conducting coculture studies on cancer cells and adipocytes. The readers will gain insight into organoid intelligence, a culmination of advancements in stem cell technologies, bioengineering, and artificial intelligence. For anyone intrigued by unraveling the mysteries of cellular life, Technologies in Cell Culture - A Journey From Basics to Advanced Applications is an indispensable resource, catering to both novice scientists and seasoned researchers seeking to expand their knowledge. Join us on this journey as we explore the myriad ways in which cell culture technology can impact biomedicine and beyond.