Even before the time of organized scientific investigation, hu mans had begun pondering and attempting to explain the work ing of the brain and the mental and behavioral states it produces. In the last twenty years there has been an almost explosive in crease in brain research. Beginning perhaps with the pioneering efforts of Francis O. Schmitt to establish the Neuroscience Re search Program and the later development of the Society for Neuroscience, there has emerged a large and powerful multi disciplinary research force devoted to understanding even the ru dimentary aspects of brain functioning. Chemists, physicists, and engineers with their special expert ise in quantitative physical measurements have teamed up with the neurobiologists, who best know the texture and design of brains, to produce particularly effective new approaches. No where is this more evident than in the recently developed meth ods like positron emission tomography and magnetic resonance imaging-techniques that allow one to observe on-going brain ac tivity in humans. This volume concerns a considerably more modest approach: the use of microelectrodes to electrochemically monitor certain aspects of chemical dynamics in functioning ani mal brain systems. The method provides signals that can be di rectly related to chemical neurotransmission. It is a relatively new technique, the first practical measurements having been made in the 1970s, and it is continuously undergoing refinement. The organizer of this book, Jay Justice, is eminently qualified for the task.

Since the first implant of a carbon microelectrode in a rat 35 years ago, there have been substantial advances in the sensitivity, selectivity and temporal resolution of electrochemical techniques. Today, these methods provide neurochemical information that is not accessible by other means. The growing recognition of the versatility of electrochemi

The new edition of this successful reference offers both cutting-edge and classic pharmacological methods. Thoroughly revised and expanded to two volumes, it offers an updated selection of the most frequently used assays for reliably detecting the pharmacological effects of potential drugs. Every chapter has been updated, and numerous assays have been added. Each of the more than 1,000 assays comprises a detailed protocol outlining purpose and rationale, and a critical assessment of the results and their pharmacological and clinical relevance.

Understanding the phenomenon of long-lasting vulnerability to addiction is essential to developing successful treatments. Written by an international team of authorities in their respective fields, Advances in the Neuroscience of Addiction provides an excellent overview of the available and emerging approaches used to investigate the biol

Now expanded and updated to include molecular biology and genetic engineering techniques. The second edition of this successful reference book contains a comprehensive selection of the most frequently used assays for reliably detecting the pharmacological effects of potential drugs. Each of the more than 1000 assays comprises a detailed protocol outlining the purpose and rationale of the method, a critical assessment of the results and their pharmacological and clinical relevance. The enclosed and fully searchable CD ROM allows easy identification of specific tests. An appendix with up-to-date guidelines and legal regulations for animal experiments in various countries will help the reader to plan experiments more effectively.

Modern neuroscience research is inherently multidisciplinary, with a wide variety of cutting edge new techniques to explore multiple levels of investigation. This Third Edition of Guide to Research Techniques in Neuroscience provides a comprehensive overview of classical and cutting edge methods including their utility, limitations, and how data are presented in the literature. This book can be used as an introduction to neuroscience techniques for anyone new to the field or as a reference for any neuroscientist while reading papers or attending talks. - Nearly 200 updated full-color illustrations to clearly convey the theory and practice of neuroscience methods - Expands on techniques from previous editions and covers many new techniques including in vivo calcium imaging, fiber photometry, RNA-Seq, brain spheroids, CRISPR-Cas9 genome editing, and more - Clear, straightforward explanations of each technique for anyone new to the field - A broad scope of methods, from noninvasive brain imaging in human subjects, to electrophysiology in animal models, to recombinant DNA technology in test tubes, to transfection of neurons in cell culture - Detailed recommendations on where to find protocols and other resources for specific techniques - "Walk-through" boxes that guide readers through experiments step-by-step

At the nexus of advances in molecular genetics and findings in redox biology, this volume elaborates on the dynamics governing cellular redox states and aggregates the body of evidence linking oxidative stress and redox modulation with a host of monogenetic and polygenetic diseases.