The Human Kidney Extracellular Matrix
Title | The Human Kidney Extracellular Matrix PDF eBook |
Author | Qingyang Kong |
Publisher | |
Pages | |
Release | 2020 |
Genre | |
ISBN |
Extracellular Matrix in the Kidney
Title | Extracellular Matrix in the Kidney PDF eBook |
Author | Hikaru Koide |
Publisher | |
Pages | 244 |
Release | 1994 |
Genre | Medical |
ISBN |
Molecular Biology of the Cell
Title | Molecular Biology of the Cell PDF eBook |
Author | |
Publisher | |
Pages | 0 |
Release | 2002 |
Genre | Cells |
ISBN | 9780815332183 |
Extracellular Matrix Biology
Title | Extracellular Matrix Biology PDF eBook |
Author | Richard O. Hynes |
Publisher | |
Pages | 0 |
Release | 2012 |
Genre | Cell interaction |
ISBN | 9781936113385 |
In most tissues, cells are surrounded by an extracellular matrix (ECM) containing proteins such as collagen, laminin, and fibronectin. The ECM plays an important role in regulating cell function. ECM proteins bind to integrins and other cell surface receptors, activating signaling pathways that regulate cellular morphology, adhesion, cell migration, cell proliferation, and apoptosis. Written and edited by experts in the field, this collection from Cold Spring Harbor Perspectives in Biology covers all aspects of ECM composition and function, as well as alterations in the ECM that occur during development, tumorigenesis, and other disease states. The contributors examine the various ECM proteins and proteoglycans, ECM receptors such as integrins, and the signaling pathways that mediate the effects of the ECM on cells. They also describe ECM functions in specific biological contexts, including angiogenesis, hemostasis, and thrombosis. Covering not only the biochemistry and cell biology of the ECM but also its roles in development, physiology, and pathology, this volume is an indispensable reference for cell biologists and all those interested in exploring the myriad functions of the ECM.
Fabrication and Characterization of an Acellular Human Kidney ECM-derived Hydrogel
Title | Fabrication and Characterization of an Acellular Human Kidney ECM-derived Hydrogel PDF eBook |
Author | Jin Xu |
Publisher | |
Pages | 35 |
Release | 2015 |
Genre | |
ISBN |
Today, more than 900,000 patients in the United States live with end-stage renal disease, with many more suffer from poor renal regeneration. Current methods commonly use synthetic tissue-engineered constructs to deliver cells, drugs, or as scaffolds for reconstruction of injured tissues. However, these constructs usually lack vascularization in vivo, and have poor nutrient diffusion abilities that limit their effectiveness. On the other hand, we have found that extracellular matrix (ECM) hydrogels can be used as scaffolds to facilitate the repair and reconstruction of various tissues. The objective of this study is to fabricate and characterize the mechanics and cell response in vitro of ECM hydrogels prepared from decellularized human kidney tissues. Our preliminary data indicate that Sodium dodecyl sulfate (SDS) decellularization of human kidney tissue sections gives the most optimal results in terms of cellular material removal and ECM structure preservation; decellularized human kidney ECM, when mixed with collagen gel on a 1:1 ratio, shows similar mechanical properties as commonly used type I collagen gel. These data suggest that decellularized human kidney ECM hydrogels are able to provide the structural support necessary for cellular activities. Given these findings and the fact that ECM hydrogels are derived from naturally occurring kidney materials, I hypothesize that decellularized human kidney ECM hydrogel is able to not only provide structural support for cell growth and proliferation, but also to enhance bioactivity and vascularization. To test this hypothesis, I performed angiogenesis and tubulogenesis studies by seeding human umbilical vein endothelial cells and human kidney microvascular endothelial cells onto or in the decellularized human kidney ECM hydrogel as well as type I collagen gel.
Differential Regulation of Extracellular Matrix Modulators by Cytokines in Human Renal Cells
Title | Differential Regulation of Extracellular Matrix Modulators by Cytokines in Human Renal Cells PDF eBook |
Author | Larine Nee |
Publisher | |
Pages | 164 |
Release | 2002 |
Genre | Cytokines |
ISBN |
Organogenesis of the Kidney
Title | Organogenesis of the Kidney PDF eBook |
Author | Lauri Saxen |
Publisher | Cambridge University Press |
Pages | 185 |
Release | 1987-04-16 |
Genre | Medical |
ISBN | 0521301521 |
Although this description of a model system for cell differentation and organogenesis emphasizes the mammalian kidney, detailed coverage is also given to the development of the transient excretory organs.