During the past three decades there has been a large amount of research on biological nitrogen fixation, in part stimulated by increasing world prices of nitrogen-containing fertilizers and environmental concerns. In the last several years, research on plant--microbe interactions, and symbiotic and asymbiotic nitrogen fixation has become truly interdisciplinary in nature, stimulated to some degree by the use of modern genetic techniques. These methodologies have allowed us to make detailed analyses of plant and bacterial genes involved in symbiotic processes and to follow the growth and persistence of the root-nodule bacteria and free-living nitrogen-fixing bacteria in soils. Through the efforts of a large number of researchers we now have a better understanding of the ecology of rhizobia, environmental parameters affecting the infection and nodulation process, the nature of specificity, the biochemistry of host plants and microsymbionts, and chemical signalling between symbiotic partners. This volume gives a summary of current research efforts and knowledge in the field of biological nitrogen fixation. Since the research field is diverse in nature, this book presents a collection of papers in the major research area of physiology and metabolism, genetics, evolution, taxonomy, ecology, and international programs.

Rhizobia are bacteria which inhabit the roots of plants in the pea family and "fix" atmospheric nitrogen for plant growth. They are thus of enormous economic importance internationally and the subject of intense research interest. Handbook for Rhizobia is a monumental book of practical methods for working with these bacteria and their plant hosts. Topics include the general microbiological properties of rhizobia and their identification, their potential as symbionts, methods for inoculating rhizobia onto plants, and molecular genetics methods for Rhizobium in the laboratory. The book will be invaluable to Rhizobium scientists, soil microbiologists, field and laboratory researchers at agricultural research centers, agronomists, and crop scientists.

The International Workshop on Biological Nitrogen Fixation for Increased Crop Productivity, Enhanced Human Health and Sustained Soil Fertility was held in June 2002 at ENSAM-INRA, Montpellier, France. The main goal of the workshop was to define research strategies for a Challenge Program initiative on Biological Nitrogen Fixation, and develop a pre-proposal based on the concept note submitted and approved by the CGIAR iSC. More than 35 participants from various scientific disciplines, research institutions and from four different continents (Africa, Asia, Americas and Europe) attended the workshop. This book, based on the proceedings of the workshop, takes a holistic approach to harnessing legume BNF technologies, starting from a socioeconomic perspective, and progressing to agronomic and genetic options, and bringing together innovative aspects and participatory research strategies.

Chemical fertilizers have had a significant impact on food production in the recent past, and are today an indispensable part of modern agriculture. On the other hand, the oil crisis of the 1970s and the current Middle East problems are constant reminders of the vulnerability of our fossil fuel dependent agriculture. There are vast areas of the developing world where N fertilizers are neither available nor affordable and, in most of these countries, balance of payment problems have resulted in the removal of N fertilizer subsidies. The external costs of environmental degradation and human health far exceed economic concerns. Input efficiency of N fertilizer is one of the lowest and, in turn, contributes substantially to environmental pollution. Nitrate in ground and surface waters and the threat to the stability of the ozone layer from gaseous oxides of nitrogen are major health and environmental concerns. The removal of large quantities of crop produce from the land also depletes soil of its native N reserves. Another concern is the decline in crop yields under continuous use of N fertilizers. These economic, environmental and production considerations dictate that biological alternatives which can augment, and in some cases replace, N fertilizers must be exploited. Long-term sustainability of agricultural systems must rely on the use and effective management of internal resources. The process of biological nitrogen fixation offers and economically attractive and ecologically sound means of reducing external nitrogen input and improving the quality and quantity of internal resources. In this book, we outline sustainability issues that dictate an increased use of biological nitrogen fixation and the constraints on its optimal use in agriculture.

Biological nitrogen fixation has essential role in N cycle in global ecosystem. Several types of nitrogen fixing bacteria are recognized: the free-living bacteria in soil or water; symbiotic bacteria making root nodules in legumes or non-legumes; associative nitrogen fixing bacteria that resides outside the plant roots and provides fixed nitrogen to the plants; endophytic nitrogen fixing bacteria living in the roots, stems and leaves of plants. In this book there are 11 chapters related to biological nitrogen fixation, regulation of legume-rhizobium symbiosis, and agriculture and ecology of biological nitrogen fixation, including new models for autoregulation of nodulation in legumes, endophytic nitrogen fixation in sugarcane or forest trees, etc. Hopefully, this book will contribute to biological, ecological, and agricultural sciences.

Phylogenetic classification of nitrogen-fixing organisms. Physiology of nitrogen fixation in free-living heterotrophs. Nitrogen fixation by photosynthetic bacteria. Nitrogen fixation in cyanobacteria. Nitrogen fixation by methanogenic bacteria. Associative nitrogen-fixing bacteria. Actinorhizal symbioses. Ecology of bradyrhizobium and rhizobium. The rhizobium infection process. Physiology of nitrogen-fixing legume nodules: compartments, and functions. Hydrogen cycling in symbiotic bacteria. Evolution of nitrogen-fixing symbioses. The rhizobium symbiosis of the nonlegume parasponia. Genetic analysis of rhizobium nodulation. Nodulins in root nodule development. Plant genetics of symbiotic nitrogen fixation. Molecular genetics of bradyrhizobium symbioses. The enzymology of molybdenum-dependent nitrogen fixation. Alternative nitrogen fixation systems. Biochemical genetics of nitrogenase. Regulation of nitrogen fixation genes in free-living and symbiotic bacteria. Isolated iron-molybdenum cofactor of nitrogenase.

Nitrogen fixation by leguminous plants is especially important when farmers are trying to minimise fertilizer use for cost or environmental reasons. This second edition of the highly successful book, first published in 1991, contains thoroughly updated and revised material on the theory and practice of nitrogen fixation in tropical cropping systems.