This manual provides a technical reference for the measurement of stable isotopes in a laboratory setting including:* A general guide to the use and application of stable isotopes in ecology and biogeochemistry* Sample collection techniques* Detailed step-by-step instructions for the preparation of samples* In-depth analysis of the instruments used to measure stable isotopes* Data processing and data interpretation

A solid introduction to stable isotopes that can also be used as an instructive review for more experienced researchers and professionals. The book approaches the use of isotopes from the perspective of ecological and biological research, but its concepts can be applied within other disciplines. A novel, step-by-step spreadsheet modeling approach is also presented for circulating tracers in any ecological system, including any favorite system an ecologist might dream up while sitting at a computer. The author’s humorous and lighthearted style painlessly imparts the principles of isotope ecology. The online material contains color illustrations, spreadsheet models, technical appendices, and problems and answers.

This book highlights new and emerging uses of stable isotope analysis in a variety of ecological disciplines. While the use of natural abundance isotopes in ecological research is now relatively standard, new techniques and ways of interpreting patterns are developing rapidly. The second edition of this book provides a thorough, up-to-date examination of these methods of research. As part of the Ecological Methods and Concepts series which provides the latest information on experimental techniques in ecology, this book looks at a wide range of techniques that use natural abundance isotopes to: follow whole ecosystem element cycling understand processes of soil organic matter formation follow the movement of water in whole watersheds understand the effects of pollution in both terrestrial and aquatic environments study extreme systems such as hydrothermal vents follow migrating organisms In each case, the book explains the background to the methodology, looks at the underlying principles and assumptions, and outlines the potential limitations and pitfalls. Stable Isotopes in Ecology and Environmental Science is an ideal resource for both ecologists who are new to isotopic analysis, and more experienced isotope ecologists interested in innovative techniques and pioneering new uses.

The emerging multidisciplinary field of earth system science sets out to improve our understanding functioning ecosystems, at a global level across the entire planet. Stable Isotopes and Biosphere - Atmosphere Interactions looks to one of its most powerful tools — the application of stable isotope analyses — to understanding biosphere-atmosphere exchange of the greenhouse gases, and synthesizes much of the recent progress in this work. Stable Isotopes and Biosphere - Atmosphere Interactions describes recent progress in understanding the mechanisms, processes and applications of new techniques. It makes a significant contribution to the emerging, multidisciplinary study of the Earth as an interacting system. This book will be an important reference for students and researchers in biology, ecology, biogeochemistry, meteorology, and atmospheric science and will be invaluable for anyone with any interest in the future of the planet. - Describes applications of new stable isotope techniques to the emerging fields of earth system science and global change - Illustrates advances in scaling of physiological processes from leaf/soil to the global scale - Contains state-of-the-art, critical reviews written by international researchers and experts

Stable isotope ratio variation in natural systems reflects the dynamics of Earth systems processes and imparts isotope labels to Earth materials. Carbon isotope ratios of atmospheric CO2 record exchange of carbon between the biosphere and the atmosphere; the incredible journeys of migrating monarchs is documented by hydrogen isotopes in their wings; and water carries an isotopic record of its source and history as it traverses the atmosphere and land surface. Through these and many other examples, improved understanding of spatio-temporal isotopic variation in Earth systems is leading to innovative new approaches to scientific problem-solving. This volume provides a comprehensive overview of the theory, methods, and applications that are enabling new disciplinary and cross-disciplinary advances through the study of "isoscapes": isotopic landscapes. "This impressive new volume shows scientists deciphering and using the natural isotope landscapes that subtly adorn our spaceship Earth.", Brian Fry, Coastal Ecology Institute, Louisiana State University, USA "An excellent timely must read and must-have reference book for anybody interested or engaged in applying stable isotope signatures to questions in e.g. Anthropology, Biogeochemistry, Ecology, or Forensic Science regarding chronological and spatial movement, changes, or distribution relating to animals, humans, plants, or water.", Wolfram Meier-Augenstein, Centre for Anatomy & Human Identification, University of Dundee, UK "Natural resources are being affected by global change, but exactly where, how, and at what pace? Isoscapes provide new and remarkably precise answers.", John Hayes, Woods Hole Oceanographic Institution, USA "This exciting volume is shaping a new landscape in environmental sciences that is utilizing the remarkable advances in isotope research to enhance and extend the capabilities of the field.", Dan Yakir, Weizmann Institute of Science, Israel

The use of Compound-specific Stable Isotope Analysis (CSIA) is increasing in many areas of science and technology for source allocation, authentication, and characterization of transformation reactions. Until now, there have been no textbooks available for students with an analytical chemical background or basic introductory books emphasising the instrumentation and theory. This book is the first to focus solely on stable isotope analysis of individual compounds in sometimes complex mixtures. It acts as both a lecture companion for students and a consultant for advanced scientists in fields including forensic and environmental science. The book starts with a brief history of the field before going on to explain stable isotopes from scratch. The different ways to express isotope abundances are introduced together with isotope effects and isotopic fractionation. A detailed account of the required technical equipment and general procedures for CSIA is provided. This includes sections on derivatization and the use of microextraction techniques in GC-IRMS. The very important topic of referencing and calibration in CSIA is clearly described. This differs from approaches used in quantitative analysis and is often difficult for the newcomer to comprehend. Examples of successful applications of CSIA in food authenticity, forensics, archaeology, doping control, environmental science, and extraterrestrial materials are included. Applications in isotope data treatment and presentation are also discussed and emphasis is placed on the general conclusions that can be drawn from the uses of CSIA. Further instrumental developments in the field are highlighted and selected experiments are introduced that may act as a basis for a short practical course at graduate level.

This book has a very strong practical orientation, telling readers what methodologies are available using stable isotopes, how studies should be designed and executed to maximise effectiveness and incisiveness in terms of data obtained and outcomes in terms of understanding events and processes in plant and ecosystem functioning. The readership is very much aimed toward postgraduates with good knowledge of general biological principles and underlying chemical and physical processes. Pre-existing knowledge of or experience in application and assay of stable isotopes is not required. The stable isotope research field is one of the most rapidly growing areas of ecophysiology and techniques using stable isotopes comprise an ever increasing component of research programs of university postgraduates and a wide range of agencies conducting environmental monitoring and rehabilitation programs. The book is tailor-made for such an audience.