This Joint ISTA and FAO Handbook provides comprehensive guidelines for the setting up and managing of seed testing laboratories, including those that cater to small- and medium-scale seed enterprises and farmers’ cooperatives, which operate in low input production systems. The adherence to the guidelines by seed testing laboratories results in the use of uniform procedures across the board thereby facilitating inter-laboratory transferability of data. This harmonization enhances confidence in seed quality assurance mechanisms. The publication is an update of a previous one that was also jointly published by both organizations in 1969 and revised in 1979 and 1983. Its utility is enhanced by a set of new up-to-date information that builds upon existing data. The Handbook provides guidance on the range of seed testing equipment, procedures, and management systems that have become available in the several decades since the previous editions. Seed testing is a critical component of the seed value chain and is essential for the production of quality seeds, i.e. seeds that are alive, can germinate and produce vigorous seedlings; are healthy and come from lots that meet set thresholds for genetic and analytical purity. The Handbook covers all aspects of seed testing, from laboratory design and equipment selection to sample preparation, testing methods, and data analysis. Importantly, the publication also addresses the challenges of testing seeds in low input systems and provides guidance on how to adapt procedures to these settings. Therefore, it serves as a reference material and training resource for everyone involved in seed quality assurance procedures, in particular the personnel of seed testing laboratories and regulatory agencies; seed producers and farmers. The updated publication represents a significant improvement over previous guidelines, as it incorporates advances in technology and provides guidance on a broader range of testing procedures. The guidelines are designed to be adaptable to different contexts and seed types and include recommendations for quality management systems, proficiency testing, and inter-laboratory comparisons, which are essential for ensuring the accuracy and reliability of seed testing results.

An essential reference for students, seed technologists, researchers, and seed industry personnel, this comprehensive guide outlines the most widely performed modern seed quality tests, explores the principles behind them, the history of seed testing, why seeds are tested and when, and sampling, sub-sampling, seed laboratory management, accreditation, and seed quality assurance programs. The authors describe statistical applications to seed testing and tolerances, and they provide a detailed morphological and structural description of seed formation and development. The book examines the testing of genetic traits and transgenic seeds, including DNA and protein genetic purity tests, and cultivar purity identification for conventional seeds. In addition to the most common seed purity and viability tests, tests for seed and seedling vigor, seed-borne diseases and seed moisture determination are also discussed.