Rapid Detection and Characterization of Foodborne Pathogens by Molecular Techniques
Title | Rapid Detection and Characterization of Foodborne Pathogens by Molecular Techniques PDF eBook |
Author | Robert E. Levin |
Publisher | CRC Press |
Pages | 582 |
Release | 2009-10-26 |
Genre | Science |
ISBN | 142009243X |
Written by one of the most prolific and respected researchers in food safety, this volume describes molecular techniques for the detection and discrimination of major infectious bacteria associated with foods. Each chapter deals with a specific organism and techniques applied to that organism. Particular focus is placed on genes associated with pathogenicity used in the polymerase chain reaction (PCR) including real-time PCR for specific detection of pathogenic bacteria and the inherent limitations of such methodology with certain pathogens. Methods for extracting microorganisms from complex food matrices andDNA purification techniques are also emphasized.
Rapid Detection, Characterization, and Enumeration of Foodborne Pathogens
Title | Rapid Detection, Characterization, and Enumeration of Foodborne Pathogens PDF eBook |
Author | J. Hoorfar |
Publisher | American Society for Microbiology Press |
Pages | 583 |
Release | 2011-04-18 |
Genre | Science |
ISBN | 1555815421 |
Detect foodborne pathogens early and minimize consumer exposure. • Presents the latest guidelines for fast, easy, cost-effective foodborne pathogen detection. • Enables readers to avoid common pitfalls and choose the most effective and efficient method, assemble the necessary resources, and implement the method seamlessly. • Includes first-hand laboratory experience from more than 85 experts from research centers across the globe.
Microarray Detection and Characterization of Bacterial Foodborne Pathogens
Title | Microarray Detection and Characterization of Bacterial Foodborne Pathogens PDF eBook |
Author | Guillermo López-Campos |
Publisher | Springer Science & Business Media |
Pages | 134 |
Release | 2012-03-12 |
Genre | Science |
ISBN | 1461432499 |
This is a review of recent advances on the use of DNA microarray for diagnosing foodborne pathogens. Rapid detection and characterization of foodborne pathogens is critical for food safety. Many relevant technologies have been intensively developed to date. DNA microarray technology offers a new way to food safety involving pathogen detection and characterization. DNA microarray can be used for detection and characterization of pathogens by analyzing hybridization patterns between capture probes and nucleic acids isolated from food samples or bacteria. It allows more rapid, accurate, and cost-effective detection of pathogens compared with traditional approaches of cultivation or immuno-assays. The application of DNA microarrays to different foodborne bacteria, such as Campylobacter, Salmonella, Listeria monocytogenes, or Shiga toxin producing Escherichia coli, will improve their rapid identification and characterization of their genetic traits (e.g., antimicrobial resistance, virulence). As bacterial foodborne diseases are posing more serious threats to public healthcare, development of rapid and accurate methods for pathogen detection and characterization is critical to their proper control at the earliest time.
Molecular Techniques in Food Biology
Title | Molecular Techniques in Food Biology PDF eBook |
Author | Aly Farag El Sheikha |
Publisher | John Wiley & Sons |
Pages | 456 |
Release | 2018-04-09 |
Genre | Technology & Engineering |
ISBN | 111937460X |
Molecular Techniques in Food Biology: Safety, Biotechnology, Authenticity & Traceability explores all aspects of microbe-food interactions, especially as they pertain to food safety. Traditional morphological, physiological, and biochemical techniques for the detection, differentiation, and identification of microorganisms have severe limitations. As an alternative, many of those responsible for monitoring food safety are turning to molecular tools for identifying foodborne microorganisms. This book reviews the latest molecular techniques for detecting, identifying, and tracing microorganisms in food, addressing both good foodborne microbes, such as those used for fermentation and in probiotics, and harmful ones responsible for foodborne illness and food quality control problems. Molecular Techniques in Food Biology: Safety, Biotechnology, Authenticity & Traceability brings together contributions by leading international authorities in food biology from academe, industry, and government. Chapters cover food microbiology, food mycology, biochemistry, microbial ecology, food biotechnology and bio-processing, food authenticity, food origin traceability, and food science and technology. Throughout, special emphasis is placed on novel molecular techniques relevant to food biology research and for monitoring and assessing food safety and quality. Brings together contributions from scientists at the leading edge of the revolution in molecular food biology Explores how molecular techniques can satisfy the dire need to deepen our understanding of how microbial communities develop in foods of all types and in all forms Covers all aspects of food safety and hygiene, microbial ecology, food biotechnology and bio-processing, food authenticity, food origin traceability, and more Fills a yawning gap in the world literature on food traceability using molecular techniques This book is an important working resource for professionals in agricultural, food science, biomedicine, and government involved in food regulation and safety. It is also an excellent reference for advanced students in agriculture, food science and food technology, biochemistry, microbiology, and biotechnology, as well as academic researchers in those fields.
Molecular Methods for the Detection and Characterization of Foodborne and Environmental Pathogens
Title | Molecular Methods for the Detection and Characterization of Foodborne and Environmental Pathogens PDF eBook |
Author | Suresh D. Pillai |
Publisher | DEStech Publications, Inc |
Pages | 168 |
Release | 2017 |
Genre | Science |
ISBN | 1605950793 |
Techniques for the detection and characterization of microbial pathogens in foods and environmental samples * Molecular techniques and their underlying theories and protocols laid out step by step * Protocols cover: sample processing, DNA extraction, PCR amplification of target genes, methods for quantifying and quality checking DNA/RNA samples * Also presents conventional microbiology, DNA, RNA and other genetic techniques using commercially available materials and kits * Guidance for examining, recording, and interpreting data. Combining the depth of a textbook and the guidance of a lab manual, this volume introduces contemporary molecular methods that are widely used to identify and characterize microbial populations and specific pathogens. The book is structured to explain the basic theory and the rationale for choosing appropriate approaches and specific methods. This material is supplemented by in-depth descriptions of lab experiments. Step-by-step guidelines are provided for a variety of experimental objectives. The techniques explained in this volume can be used in a variety of instructional settings and have been taught to students in academic disciplines such as food science, nutrition, environmental technology, and animal science.
Rapid Detection, Characterization and Enumeration of Foodborne Pathogens
Title | Rapid Detection, Characterization and Enumeration of Foodborne Pathogens PDF eBook |
Author | Jeffrey Hoorfar |
Publisher | |
Pages | 0 |
Release | 2011 |
Genre | |
ISBN |
Industrial and Environmental Biotechnology
Title | Industrial and Environmental Biotechnology PDF eBook |
Author | Nuzhat Ahmed |
Publisher | Horizon Press |
Pages | 0 |
Release | 2014-04-14 |
Genre | Science |
ISBN | 9781898486527 |
The contamination of the environment by herbicides, pesticides, solvents, various industrial byproducts (including toxic metals, radionucleotides and metalloids) is of enormous economic and environmental significance. Biotechnology can be used to develop "green" or environmentally friendly solutions to these problems by harnessing the ability of bacteria to adapt metabolic pathways, or recruit new genes to metabolise harmful compounds into harmless byproducts. In addition to its role in cleaning-up the environment, biotechnology can be used for the production of novel compounds with both agricultural and industrial applications. Internationally acclaimed authors from diverse fields present comprehensive reviews of all aspects of Industrial and Environmental Biotechnology. Chapters concerned with environmental biotechnology cover two major categories of pollutants: organic compounds and metals. Organic pollutants include cyclic aromatic compounds, with/without nitrogenous or chloride substitutions while metal pollutants include copper, chromate, silver, arsenic and mercury. The genetic basis of bioremediation and the microbial processes involved are examined, and the current and/or potential applications of bioremediation are discussed. The use of biotechnology for industrial and agricultural applications includes a chapter on the use of enzymes as biocatalysts to synthesize novel opiate derivatives of medical value. The conversion of low-value molasses to higher value products by biotechnological methods and the use tissue culture methods to improve sugar cane and potatoes crop production is discussed.