Test No. 489: In Vivo Mammalian Alkaline Comet Assay
Title | Test No. 489: In Vivo Mammalian Alkaline Comet Assay PDF eBook |
Author | OECD |
Publisher | OECD Publishing |
Pages | 25 |
Release | 2014-09-26 |
Genre | |
ISBN | 9264224173 |
The in vivo alkaline single cell gel electrophoresis assay, also called alkaline Comet Assay is a method measuring DNA strand breaks in eukaryotic cells.
OECD Guidelines for the Testing of Chemicals, Section 4 Test No. 489: In Vivo Mammalian Alkaline Comet Assay
Title | OECD Guidelines for the Testing of Chemicals, Section 4 Test No. 489: In Vivo Mammalian Alkaline Comet Assay PDF eBook |
Author | OECD |
Publisher | OECD Publishing |
Pages | 27 |
Release | 2016-07-29 |
Genre | |
ISBN | 9264264884 |
The in vivo alkaline single cell gel electrophoresis assay, also called alkaline Comet Assay is a method measuring DNA strand breaks in eukaryotic cells.
OECD Guidelines for the Testing of Chemicals, Section 4 Test No. 470: Mammalian Erythrocyte Pig-a Gene Mutation Assay
Title | OECD Guidelines for the Testing of Chemicals, Section 4 Test No. 470: Mammalian Erythrocyte Pig-a Gene Mutation Assay PDF eBook |
Author | OECD |
Publisher | OECD Publishing |
Pages | 37 |
Release | 2022-06-30 |
Genre | |
ISBN | 9264366067 |
This Test Guideline describes an in vivo erythrocyte Pig-a gene mutation assay (hereafter called the Pig-a assay) which uses an endogenous mammalian gene, the phosphatidylinositol glycan class A gene (Pig-a), as a reporter of somatic-cell gene mutation. In vivo gene mutation tests, such as the Pig-a assay, are especially relevant for assessing mutagenicity because physiological factors, such as absorption of the test chemical from the site of exposure, distribution of the test chemical throughout the test system via systemic circulation, and in vivo metabolism and DNA repair processes, all contribute to the mutagenic responses.
OECD Guidelines for the Testing of Chemicals, Section 4 Test No. 482: Genetic Toxicology: DNA Damage and Repair, Unscheduled DNA Synthesis in Mammalian Cells in vitro
Title | OECD Guidelines for the Testing of Chemicals, Section 4 Test No. 482: Genetic Toxicology: DNA Damage and Repair, Unscheduled DNA Synthesis in Mammalian Cells in vitro PDF eBook |
Author | OECD |
Publisher | OECD Publishing |
Pages | 7 |
Release | 1986-10-23 |
Genre | |
ISBN | 926407144X |
The Test Guideline for Unscheduled DNA Synthesis (UDS) in mammalian cells in vitro describes procedures utilizing primary cultures, human lymphocytes or established cell lines, to detect DNA repair synthesis after excision and removal of a stretch ...
OECD Guidelines for the Testing of Chemicals, Section 4 Test No. 490: In Vitro Mammalian Cell Gene Mutation Tests Using the Thymidine Kinase Gene
Title | OECD Guidelines for the Testing of Chemicals, Section 4 Test No. 490: In Vitro Mammalian Cell Gene Mutation Tests Using the Thymidine Kinase Gene PDF eBook |
Author | OECD |
Publisher | OECD Publishing |
Pages | 24 |
Release | 2016-07-29 |
Genre | |
ISBN | 9264264906 |
This TG includes two distinct in vitro mammalian gene mutation assays requiring two specific tk heterozygous cells lines: L5178Y tk+/-3.7.2C cells for the mouse lymphoma assay (MLA) and TK6 tk+/- cells for the TK6 assay.
OECD Guidelines for the Testing of Chemicals, Section 4 Test No. 473: In Vitro Mammalian Chromosomal Aberration Test
Title | OECD Guidelines for the Testing of Chemicals, Section 4 Test No. 473: In Vitro Mammalian Chromosomal Aberration Test PDF eBook |
Author | OECD |
Publisher | OECD Publishing |
Pages | 22 |
Release | 2016-07-29 |
Genre | |
ISBN | 9264264647 |
This test identifies agents that cause structural chromosome aberrations in cultured mammalian somatic cells.
OECD Guidelines for the Testing of Chemicals, Section 4 Test No. 486: Unscheduled DNA Synthesis (UDS) Test with Mammalian Liver Cells in vivo
Title | OECD Guidelines for the Testing of Chemicals, Section 4 Test No. 486: Unscheduled DNA Synthesis (UDS) Test with Mammalian Liver Cells in vivo PDF eBook |
Author | OECD |
Publisher | OECD Publishing |
Pages | 8 |
Release | 1997-07-21 |
Genre | |
ISBN | 9264071520 |
The purpose of the unscheduled DNA synthesis (UDS) test with mammalian liver cells in vivo is to identify substances that induce DNA repair after excision and removal of a stretch of DNA containing a region of damage induced by chemical substances ...