OECD Guidelines for the Testing of Chemicals, Section 4 Test No. 470: Mammalian Erythrocyte Pig-a Gene Mutation Assay
Title | OECD Guidelines for the Testing of Chemicals, Section 4 Test No. 470: Mammalian Erythrocyte Pig-a Gene Mutation Assay PDF eBook |
Author | OECD |
Publisher | OECD Publishing |
Pages | 37 |
Release | 2022-06-30 |
Genre | |
ISBN | 9264366067 |
This Test Guideline describes an in vivo erythrocyte Pig-a gene mutation assay (hereafter called the Pig-a assay) which uses an endogenous mammalian gene, the phosphatidylinositol glycan class A gene (Pig-a), as a reporter of somatic-cell gene mutation. In vivo gene mutation tests, such as the Pig-a assay, are especially relevant for assessing mutagenicity because physiological factors, such as absorption of the test chemical from the site of exposure, distribution of the test chemical throughout the test system via systemic circulation, and in vivo metabolism and DNA repair processes, all contribute to the mutagenic responses.
Test No. 476: In Vitro Mammalian Cell Gene Mutation Tests using the Hprt and xprt genes
Title | Test No. 476: In Vitro Mammalian Cell Gene Mutation Tests using the Hprt and xprt genes PDF eBook |
Author | OECD |
Publisher | OECD Publishing |
Pages | 18 |
Release | 2015-07-28 |
Genre | |
ISBN | 9264243089 |
The in vitro mammalian cell gene mutation test can be used to detect gene mutations induced by chemical substances. In this test, the used genetic endpoints measure mutation at hypoxanthine-guanine phosphoribosyl transferase (HPRT), and at a transgene of xanthineguanine phosphoribosyl ...
Test No. 490: In Vitro Mammalian Cell Gene Mutation Tests Using the Thymidine Kinase Gene
Title | Test No. 490: In Vitro Mammalian Cell Gene Mutation Tests Using the Thymidine Kinase Gene PDF eBook |
Author | OECD |
Publisher | OECD Publishing |
Pages | 25 |
Release | 2015-07-28 |
Genre | |
ISBN | 9264242244 |
This TG includes two distinct in vitro mammalian gene mutation assays requiring two specific tk heterozygous cells lines: L5178Y tk+/-3.7.2C cells for the mouse lymphoma assay (MLA) and TK6 tk+/- cells for the TK6 assay.
Test No. 488: Transgenic Rodent Somatic and Germ Cell Gene Mutation Assays
Title | Test No. 488: Transgenic Rodent Somatic and Germ Cell Gene Mutation Assays PDF eBook |
Author | OECD |
Publisher | OECD Publishing |
Pages | 16 |
Release | 2011-07-28 |
Genre | |
ISBN | 9264122818 |
This Test Guideline describes an in vivo assay that detects chemicals that may induce gene mutations. In this assay, transgenic rats or mice that contain multiple copies of chromosomally integrated plasmid or phage shuttle vectors are used. The ...
Test No. 474: Mammalian Erythrocyte Micronucleus Test
Title | Test No. 474: Mammalian Erythrocyte Micronucleus Test PDF eBook |
Author | OECD |
Publisher | OECD Publishing |
Pages | 21 |
Release | 2014-09-26 |
Genre | |
ISBN | 9264224297 |
The mammalian in vivo micronucleus test detects damage induced by the test substance to the chromosomes or the mitotic apparatus of erythroblasts, by analysis of erythrocytes as sampled in bone marrow and/or peripheral blood cells of animals.
OECD Guidelines for the Testing of Chemicals, Section 4 Test No. 485: Genetic toxicology, Mouse Heritable Translocation Assay
Title | OECD Guidelines for the Testing of Chemicals, Section 4 Test No. 485: Genetic toxicology, Mouse Heritable Translocation Assay PDF eBook |
Author | OECD |
Publisher | OECD Publishing |
Pages | 6 |
Release | 1986-10-23 |
Genre | |
ISBN | 9264071504 |
The mouse heritable translocation test detects structural and numerical chromosome changes in mammalian germ cells as recovered in first generation progeny. The types of chromosome changes detected in this test system are reciprocal translocations ...
OECD Guidelines for the Testing of Chemicals, Section 4 Test No. 480: Genetic Toxicology: Saccharomyces cerevisiae, Gene Mutation Assay
Title | OECD Guidelines for the Testing of Chemicals, Section 4 Test No. 480: Genetic Toxicology: Saccharomyces cerevisiae, Gene Mutation Assay PDF eBook |
Author | OECD |
Publisher | OECD Publishing |
Pages | 5 |
Release | 1986-10-23 |
Genre | |
ISBN | 9264071407 |
This assay may be used to measure gene mutation in yeast, a eukaryotic micro-organism. Strains of Saccharomyces cerevisiae have been developed which detect forward or reverse mutations. A variety of haploid and diploid strains of the yeast can be ...