This Test Guideline describes the HPLC method, which permits to determine the partition coefficient (n-octanol/water) (Pow). Reverse phase HPLC is performed on analytical columns packed with a solid phase containing long hydrocarbon chains ...

This Test Guideline describes an experimental method using HPLC for the estimation of the adsorption coefficient Koc in soil and in sewage sludge. The adsorption coefficient is defined as the ratio between the concentration of the substance in the ...

This Test Guideline describes a procedure for characterising the bioconcentration potential of substances in fish, using an aqueous (standard and minimised tests) or dietary exposure, under flow-through conditions (but semi-static regimes are ...

The Test Guideline (TG) describes the use of liver S9 sub-cellular fraction (RT-S9) of rainbow trout (Oncorhynchus mykiss) as a metabolising system to determine the clearance (CL, IN VITRO, INT ) of a test chemical using a substrate depletion approach. Introduction of the test chemical to the ...

The Test Guideline (TG) describes the use of cryopreserved rainbow trout (Oncorhynchus mykiss) hepatocytes (RT-HEP) as a metabolising system to determine the clearance (CL, IN VITRO, INT) of a test chemical using a substrate depletion approach. Introduction of the test chemical to the RT-HEP ...

This Test Guideline describes a method to assess the extent and kinetics of primary and ultimate biodegradation of organic chemicals whose route of entry into the environment begins with their discharge to wastewater. It consists of five simulation ...

This up-to-date, comprehensive toxicology handbook is devoted to the effects of environmental pollution on fish. Fish species represent nearly half of all vertebrates and have become important sentinels for environmental contamination and model organisms for understanding adverse outcomes from exposures. This new edition is written by recognized experts, and it highlights the significant research progress in fish toxicology that has resulted from rapid technological developments in analytical, biochemical, and genomic sciences. The book: Discusses fundamental topics such as toxicokinetics in fishes, processes governing biotransformation within these organisms, and reactive oxygen species and oxidative stress. Explains key target organ systems for chemical impacts in fish, such as the nervous and immune systems, and how fishes can develop resistance to chemical toxicity. Covers multi-transgenerational effects on fishes, epigenetics, proteomics and metabolomics, and adverse outcome pathways. Replacing the case studies in the first edition, this update delves into the impacts of microplastics, pharmaceuticals, and oil spills in dedicated final chapters. With nearly 200 illustrations and tables, this comprehensive reference work presents concepts in a way that is useful for both novices to and experts in the field of fish toxicology.