Protein NMR Spectroscopy
Title | Protein NMR Spectroscopy PDF eBook |
Author | John Cavanagh |
Publisher | Elsevier |
Pages | 915 |
Release | 2010-07-21 |
Genre | Science |
ISBN | 008047103X |
Protein NMR Spectroscopy, Second Edition combines a comprehensive theoretical treatment of NMR spectroscopy with an extensive exposition of the experimental techniques applicable to proteins and other biological macromolecules in solution. Beginning with simple theoretical models and experimental techniques, the book develops the complete repertoire of theoretical principles and experimental techniques necessary for understanding and implementing the most sophisticated NMR experiments. Important new techniques and applications of NMR spectroscopy have emerged since the first edition of this extremely successful book was published in 1996. This updated version includes new sections describing measurement and use of residual dipolar coupling constants for structure determination, TROSY and deuterium labeling for application to large macromolecules, and experimental techniques for characterizing conformational dynamics. In addition, the treatments of instrumentation and signal acquisition, field gradients, multidimensional spectroscopy, and structure calculation are updated and enhanced. The book is written as a graduate-level textbook and will be of interest to biochemists, chemists, biophysicists, and structural biologists who utilize NMR spectroscopy or wish to understand the latest developments in this field. Provides an understanding of the theoretical principles important for biological NMR spectroscopy Demonstrates how to implement, optimize and troubleshoot modern multi-dimensional NMR experiments Allows for the capability of designing effective experimental protocols for investigations of protein structures and dynamics Includes a comprehensive set of example NMR spectra of ubiquitin provides a reference for validation of experimental methods
Fundamentals of Protein NMR Spectroscopy
Title | Fundamentals of Protein NMR Spectroscopy PDF eBook |
Author | Gordon S. Rule |
Publisher | Springer Science & Business Media |
Pages | 543 |
Release | 2006-02-16 |
Genre | Science |
ISBN | 1402035004 |
NMR spectroscopy has proven to be a powerful technique to study the structure and dynamics of biological macromolecules. Fundamentals of Protein NMR Spectroscopy is a comprehensive textbook that guides the reader from a basic understanding of the phenomenological properties of magnetic resonance to the application and interpretation of modern multi-dimensional NMR experiments on 15N/13C-labeled proteins. Beginning with elementary quantum mechanics, a set of practical rules is presented and used to describe many commonly employed multi-dimensional, multi-nuclear NMR pulse sequences. A modular analysis of NMR pulse sequence building blocks also provides a basis for understanding and developing novel pulse programs. This text not only covers topics from chemical shift assignment to protein structure refinement, as well as the analysis of protein dynamics and chemical kinetics, but also provides a practical guide to many aspects of modern spectrometer hardware, sample preparation, experimental set-up, and data processing. End of chapter exercises are included to emphasize important concepts. Fundamentals of Protein NMR Spectroscopy not only offer students a systematic, in-depth, understanding of modern NMR spectroscopy and its application to biomolecular systems, but will also be a useful reference for the experienced investigator.
Protein NMR Spectroscopy
Title | Protein NMR Spectroscopy PDF eBook |
Author | Lu-Yun Lian |
Publisher | John Wiley & Sons |
Pages | 345 |
Release | 2011-06-09 |
Genre | Science |
ISBN | 1119972825 |
Nuclear Magnetic Resonance (NMR) spectroscopy, a physical phenomenon based upon the magnetic properties of certain atomic nuclei, has found a wide range of applications in life sciences over recent decades. This up-to-date volume covers NMR techniques and their application to proteins, with a focus on practical details. Providing newcomers to NMR with practical guidance to carry out successful experiments with proteins and analyze the resulting spectra, those familiar with the chemical applications of NMR will also find it useful in understanding the special requirements of protein NMR.
Protein NMR Techniques
Title | Protein NMR Techniques PDF eBook |
Author | Alexander Shekhtman |
Publisher | Humana Press |
Pages | 534 |
Release | 2016-05-01 |
Genre | |
ISBN | 9781493961955 |
In its expanded third edition, this Methods in Molecular Biology volume offers techniques for NMR sample preparation, solution and solid state NMR methodologies and data processing, materials lists, step-by-step protocols, troubleshooting tips and more."
Intrinsically Disordered Proteins Studied by NMR Spectroscopy
Title | Intrinsically Disordered Proteins Studied by NMR Spectroscopy PDF eBook |
Author | Isabella C. Felli |
Publisher | Springer |
Pages | 428 |
Release | 2015-09-19 |
Genre | Science |
ISBN | 3319201646 |
This book discusses the paradigm-shifting phenomenon of intrinsically disordered proteins (IDPs) and hybrid proteins containing ordered domains and functional IDP regions (IDPRs). The properties of IDPs and IDPRs are highly complementary to those deriving from the presence of a unique and well-defined three-dimensional fold. Ignored for a long time in high-resolution studies of proteins, intrinsic protein disorder is now recognized as one of the key features for a large variety of cellular functions, where structural flexibility presents a functional advantage in terms of binding plasticity and promiscuity and this volume explores this exciting new research. Recent progress in the field has radically changed our perspective to study IDPs through NMR: increasingly complex IDPs can now be characterized, a wide range of observables can be determined reporting on the structural and dynamic properties, computational methods to describe the structure and dynamics are in continuous development and IDPs can be studied in environments as complex as whole cells. This volume communicates the new exciting possibilities offered by NMR and presents open questions to foster further developments. Intrinsically Disordered Proteins Studied by NMR Spectroscopy provides a snapshot to researchers entering the field as well as providing a current overview for more experienced scientists in related areas.
Protein NMR
Title | Protein NMR PDF eBook |
Author | Ranajeet Ghose |
Publisher | |
Pages | 446 |
Release | 2018 |
Genre | Medicine |
ISBN | 9781493973866 |
This volume covers state-of-the-art applications of solid-state and solution nuclear magnetic resonance( NMR) spectroscopy to study protein structure, dynamics and interactions. Chapters detail various aspects of data acquisition and processing, determination of the structure, multi-timescale dynamics of entities ranging from individual proteins to large macromolecular complexes to intact viral assemblies. The final two chapters will highlight the promise of NMR beyond field strengths of 1 GHz to study the structure, dynamics and interactions of a larger class of proteins and protein complexes of extraordinary biological interest. Written in the highly successful Methods in Molecular Biology series format, chapters provide detailed laboratory protocols and troubleshooting tips that would be of great practical help to NMR spectroscopists with different levels of expertise.
NMR of Proteins and Nucleic Acids
Title | NMR of Proteins and Nucleic Acids PDF eBook |
Author | Kurt Wüthrich |
Publisher | Wiley-Interscience |
Pages | 330 |
Release | 1986 |
Genre | Medical |
ISBN |
Lists of tables. The foundations: structure and NMR of biopolymers. Resonance assignments and structure determination in proteins. Resonance assignments and structure determiantion in nucleic acids. With NMR to biopolymer conformation and beyond.