Molecular Zoology Advances, Strategies, and Protocols Edited by Joan D. Ferraris and Stephen R. Palumbi Contemporary tools of molecular biology continue to open new areas of biological research and to provide important answers to classic problems. Zoological questions of mating strategies, physiological adaptation, genetic exchange between populations, cell lineages during development, and many others are now being powerfully addressed using tools from the molecular arsenal. To provide broad access to these tools requires an authoritative reference that highlights recent advances, lays out future strategies, and provides working protocols to a wide audience of zoological scientists. Molecular Zoology: Advances, Strategies, and Protocols outlines the core concepts of these critical molecular techniques and provides specific instructions for their use. The book is divided into two main parts: Research Strategies and Protocols. The first section features detailed descriptions of the research approaches that incorporate molecular tools in the study of developmental, physiological, ecological, and evolutionary processes. In addition to charting recent advances, this section shows how to interpret results and describes the advantages and disadvantages of alternative approaches. These chapters function as entry points to molecular zoology for broadly trained zoologists without formal molecular training, graduate students, and molecular biologists in other fields. The second section is a compilation of over 60 protocols which have been developed, tested, and perfected by leading researchers in the field. It provides step-by-step coverage of each protocol, featuring for each a summary of its underlying rationale, a list of necessary reagents and solutions, and a discussion of potential obstacles to a particular technique. Specific techniques covered in the book include: * Applications of parametric bootstrapping in molecular phylogenetics * Microsatellite analysis of genetic mating systems and genetic relatedness * Use of RAPD-PCR markers in genetic structure and genealogies * PCR-based cloning across large taxonomic distances * Cell lineage analysis using retroviral vectors * Osmoregulatory gene characterization and expression * Regulatory element identification and transcription factor analysis * Protocols for in situ hybridization, DNA footprinting, gene knockout, ribonuclease protection assay, and coupled transcription/translation reactions. Molecular Zoology: Advances, Strategies, and Protocols is an authoritative resource designed to provide both basic and in-depth explanations of molecular investigation procedures for research scientists in all areas of organismal and integrative biology, including zoology, marine biology, and ecology. With its extensive coverage of molecular protocols, graduate students in biology will also find this book to be an indispensable manual for laboratory work.

The process whereby a single cell, the fertilized egg, develops into an adult has fascinated for centuries. Great progress in understanding that process, h- ever, has been made in the last two decades, when the techniques of molecular biology have become available to developmental biologists. By applying these techniques, the exact nature of many of the interactions responsible for forming the body pattern are now being revealed in detail. Such studies are a large, and it seems ever-expanding, part of most life-science groups. It is at newcomers to this field that this book is primarily aimed. A number of different plants and animals serve as common model org- isms for developmental studies. In Molecular Methods in Developmental Bi- ogy: Xenopus and Zebrafish, a range of the molecular methods applicable to two of these organisms are described, these are the South African clawed frog, Xenopus laevis, and the zebrafish, Brachydanio rerio. The embryos of both of these species develop rapidly and externally, making them particularly suited to investigations of early vertebrate development. However, both Xenopus and zebrafish have their own advantages and disadvantages. Xenopus have large, robust embryos that can be manipulated surgically with ease, but their pseudotetraploidy and long generation time make them unsuitable candidates for genetics. This disadvantage may soon be overcome by using the diploid Xenopus tropicalis, and early experiments are already underway. The transp- ent embryos of zebrafish render them well-suited for in situ hybridization and immunohistochemistry, and good for observing mutations in genetic screens.

Plant Cell Biology, Second Edition: From Astronomy to Zoology connects the fundamentals of plant anatomy, plant physiology, plant growth and development, plant taxonomy, plant biochemistry, plant molecular biology, and plant cell biology. It covers all aspects of plant cell biology without emphasizing any one plant, organelle, molecule, or technique. Although most examples are biased towards plants, basic similarities between all living eukaryotic cells (animal and plant) are recognized and used to best illustrate cell processes. This is a must-have reference for scientists with a background in plant anatomy, plant physiology, plant growth and development, plant taxonomy, and more. - Includes chapter on using mutants and genetic approaches to plant cell biology research and a chapter on -omic technologies - Explains the physiological underpinnings of biological processes to bring original insights relating to plants - Includes examples throughout from physics, chemistry, geology, and biology to bring understanding on plant cell development, growth, chemistry and diseases - Provides the essential tools for students to be able to evaluate and assess the mechanisms involved in cell growth, chromosome motion, membrane trafficking and energy exchange

"This book is an introductory course in molecular biology for mathematicians, physicists, and engineers. It covers the basic features of DNA, proteins, and cells but in the context of recent technological advances, such as next-generation sequencing and high-throughput screens, and their applications. This enables readers to move rapidly from the b

First published in 1966 as a 60th birthday tribute to Max Delbrck, this influential work is republished as "The Centennial Edition." The book was hailed as "[introducing] into the literature of science, for the first time, a self-conscious historical element in which the participants in scientific discovery engage in writing their own chronicle ("Journal of History of Biology").