Molecular Characterization of Pluripotency in Embryos and Embryonic Stem Cells
Title | Molecular Characterization of Pluripotency in Embryos and Embryonic Stem Cells PDF eBook |
Author | Josep Pareja Gómez |
Publisher | |
Pages | 228 |
Release | 2010 |
Genre | |
ISBN | 9788469456811 |
Pluripotent Stem Cells
Title | Pluripotent Stem Cells PDF eBook |
Author | Uma Lakshmipathy |
Publisher | Humana Press |
Pages | 0 |
Release | 2013-04-02 |
Genre | Science |
ISBN | 9781627033473 |
Human pluripotent stem cells such as human embryonic stem cells (hESC) and induced pluripotent stem cells (iPSC) with their unique developmental plasticity hold immense potential as cellular models for drug discovery and in regenerative medicine as a source for cell replacement. While hESC are derived from a developing embryo, iPSC are generated with forced expression of key transcription factors to convert adult somatic cells to ESC-like cells, a process termed reprogramming. Using iPSC overcomes ethical issues concerning the use of developing embryos and it can be generated from patient-specific or disease-specific cells for downstream applications. Pluripotent Stem Cells: Methods and Protocols highlights the best methods and systems for the entire work flow. Divided into four convenient sections, topics include a focus on producing iPSC from diverse somatic sources, media systems for expanding ESC and iPSC with detailed protocols for directed differentiation into specific lineages, commonly used cellular and molecular characterization methods , and the potential application of labeled stem cells with specific methods for cloning, gene delivery and cell engineering. Written in the successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Pluripotent Stem Cells: Methods and Protocols seeks to serve both professionals and novices with its well-honed methodologies in an effort to further our knowledge of this essential cellular feature.
Embryonic Stem Cells
Title | Embryonic Stem Cells PDF eBook |
Author | Craig Atwood |
Publisher | BoD – Books on Demand |
Pages | 692 |
Release | 2011-04-26 |
Genre | Science |
ISBN | 9533071966 |
Pluripotency is a prerequisite for the subsequent coordinated differentiation of embryonic stem cells into all tissues of the body. This book describes recent advances in our understanding of pluripotency and the hormonal regulation of embryonic stem cell differentiation into tissue types derived from the ectoderm, mesoderm and endoderm.
Generation and Molecular Characterization of Mouse Embryonic Stem Cells Derived Trunk-like Structures
Title | Generation and Molecular Characterization of Mouse Embryonic Stem Cells Derived Trunk-like Structures PDF eBook |
Author | Adriano Bolondi |
Publisher | |
Pages | 0 |
Release | 2022 |
Genre | |
ISBN |
Embryonic Stem Cell Protocols
Title | Embryonic Stem Cell Protocols PDF eBook |
Author | Kursad Turksen |
Publisher | Springer Nature |
Pages | 363 |
Release | 2022-07-25 |
Genre | Science |
ISBN | 1071624377 |
This fully updated volume explores improved and extended protocols for embryonic stem cell (ESC) research, provided with the most direct and informative methodologies currently available. The book examines how these models for cell lineage and differentiation studies have continued to mature into a critical research workhorse. Written for the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Embryonic Stem Cell Protocols, Fourth Edition serves as an ideal resource for researching mining the depths of the ESC field.
Human Naïve Pluripotent Stem Cells
Title | Human Naïve Pluripotent Stem Cells PDF eBook |
Author | Peter Rugg-Gunn |
Publisher | Humana |
Pages | 288 |
Release | 2021-12-07 |
Genre | Science |
ISBN | 9781071619070 |
This volume provides readers with a comprehensive collection of methods to guide them on how to generate, characterize, and use naïve human pluripotent stem cells (hPSCs). The chapters in this book cover topics such as three predominant routes to generate naïve hPSC lines; methods to differentiate naïve hPSCs into specialized cell types; and techniques to characterize naïve hPSCs using key molecular landmarks that benchmark and quality control the cell lines. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Human Naïve Pluripotent Stem Cells: Methods and Protocols is a valuable resource for novice and expert researchers who are looking to learn more or expand their research in this developing field.
Gametogenesis, Early Embryo Development and Stem Cell Derivation
Title | Gametogenesis, Early Embryo Development and Stem Cell Derivation PDF eBook |
Author | Tiziana A.L. Brevini |
Publisher | Springer Science & Business Media |
Pages | 77 |
Release | 2012-09-30 |
Genre | Science |
ISBN | 1461455324 |
This Brief offers a concise, handy overview of the main concepts related to Embryology, revisited through the novel concepts that are applied daily in stem cell research and cell therapy oriented investigations. It is based on three main areas: -The process involved in female gamete differentiation and maturation. The main aspects related to cell biology will be covered and an overview of the epigenetic regulation of gametogenesis will be presented. -Early stages of embryo development with a careful analysis of the regulatory mechanisms driving cleavage, polarization and genome activation. -Stem cell and gametogenesis. The use of the oocyte as a possible source for the derivation of stem cell lines is discussed and depicted as a powerful tool to investigate oocyte potency and asymmetric imprinting. The potential biological implications are evaluated and use of stem cells to derive oocytes is presented.