In terrestrial ecosystems, soil microorganisms and soil animals are essential for litter degradation, soil formation and the availability of nutrients and trace elements. The measurement of biological soil parameters allows a rapid evaluation of the effects of chemical and physical influences due to pollutants or soil management. This book introduces a number of well proved methods for the analysis of carbon, nitrogen, phosphorus and sulfur cycles. It focuses further on the determination of the number and biomass of microorganisms, algae and animals in the soil. Particular emphasis is placed on the comprehensible and complete description of the experimental procedures.

The fourth edition of Soil Microbiology, Ecology and Biochemistry updates this widely used reference as the study and understanding of soil biota, their function, and the dynamics of soil organic matter has been revolutionized by molecular and instrumental techniques, and information technology. Knowledge of soil microbiology, ecology and biochemistry is central to our understanding of organisms and their processes and interactions with their environment. In a time of great global change and increased emphasis on biodiversity and food security, soil microbiology and ecology has become an increasingly important topic. Revised by a group of world-renowned authors in many institutions and disciplines, this work relates the breakthroughs in knowledge in this important field to its history as well as future applications. The new edition provides readable, practical, impactful information for its many applied and fundamental disciplines. Professionals turn to this text as a reference for fundamental knowledge in their field or to inform management practices. - New section on "Methods in Studying Soil Organic Matter Formation and Nutrient Dynamics" to balance the two successful chapters on microbial and physiological methodology - Includes expanded information on soil interactions with organisms involved in human and plant disease - Improved readability and integration for an ever-widening audience in his field - Integrated concepts related to soil biota, diversity, and function allow readers in multiple disciplines to understand the complex soil biota and their function

This book presents a wide range of biotechnological methods for application in soil microbiology analysis, including all essential methods involving molecular biology, immunology, microbiology, and structural biology, such as transcriptome analysis, RNAi technology, molecular matchmaking, RAPD, T-RFLP and FT/MS. The techniques and procedures presented here offer practical guides for immediate use in the laboratory. This volume will be of use both to the first-timer and to the experienced scientist.

This practical handbook describes sampling and laboratory assessment methods for the biodiversity of a number of key functional groups of soil organisms, including insects, earthworms, nematodes, fungi and bacteria. The methods have been assembled and the protocols drafted by a number of scientists associated with the UNEP-GEF funded Conservation and Sustainable Management of Below-Ground Biodiversity Project, executed by the Tropical Soil Biology and Fertility (TSBF) Institute of the International Center for Tropical Agriculture (CIAT). The methods provide a standardized basis for characterizing soil biodiversity and current land uses in terrestrial natural, semi-natural and agroecosystems in tropical forests and at forest margins. The aim is to assess soil biodiversity against current and historic land use practices both at plot and landscape scales and, further, to identify opportunities for improved sustainable land management through the introduction, management or remediation of soil biota, thus reducing the need for external inputs such as fertilizers and pesticides. The book also contains extensive advice on the handling of specimens and the allocation of organisms to strain or functional group type. Published with TSBF-CIAT, CTA, UNEP and GEF

Quality control and quality assurance in applied soil microbiology and biochemistry. Soil sampling, handling, storage and analysis. Enrichment, isolation and counting of soil microrganisms. Anaerobic microbial activities in soil. Enzyme activities. Microbial biomass. Community structure. Field methods. Bioremediation of soil.

Soil sampling for microbiological analysis; Statistical treatment of microbial data; Soil sterilization; Soil water potencial; Most probable number counts; Light microscopic methods for studying soil microorganisms; Viruses; Recovery and enumeration of viable bacteria; Coliform bacteria; Autotrophic nitrifying bacteria; Free-living dinitrogen-fixing bacteria; Legume nodule symbionts; Anaerobic bacteria and processes; Denitrifiers; Actiomycetes; Frankia and the actinorhizal symbiosis; Filamentous fungi; Vesicular-arbuscular mycorrhizal fungi; Isolation of microorganisms producting antibiotics; Microbiological procedures for biodegradation research; Algae and cyanobacteria; Marking soil bacteria with lacZY; Detection of specific DNA sequences in environmental sample via polymerase chaim reaction; Isolation and purification of bacterial DNA from soil; Microbial biomass; Soil enzymes; Carbon mineralization; Isotopic methods for the study of soil organic matter dynamics ; Practical considerations in the use of nitrogen tracers in agricultural and environmental research; Nitrogen availability; Nitrogen mineralization, immobilization, and nitrification; Dinitrogen fixation; Measuring denitrification in the field; Sulfur oxidation and reduction in soils; Iron and manganese oxidation and reduction.

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