Viruses are major pathogens in humans, and in the organisms with which we share this planet. The massive health and economic burden these agents impose has spurred a huge research effort to understand their most intimate details. One outcome of this effort has been the production, in many but certainly not all cases, of effective vaccines and therapies. - other consequence has been the realization that we can exploit viruses and put them to work on our behalf. Viruses are still seen to have the most - tential as vehicles for gene delivery and other therapeutic applications. However, their ability to exploit cellular functions to their own ends makes viruses not only highly effective pathogens but also exquisite experimental tools. Work with viruses underpins much of our current understanding of molecular cell biology and related fields. For membrane traffic in parti- lar, viruses have been crucial in providing insights into key cellular fu- tions and the molecular mechanisms underlying these events.

This book summarizes all new data obtained after development of methods of Golgi complex sub fractionation, molecular biology and microscopy. It collects the full range of expertise, different points of view and different approaches. The book is devoted to molecular modes of the function of the Golgi apparatus as a whole, taking into account all experimental data. The book aims to make the functional organization of the Golgi apparatus more understandable.

This book covers the past, present and future of the intra-cellular trafficking field, which has made a quantum leap in the last few decades. It details how the field has developed and evolved as well as examines future directions.

The ability of viruses to exploit cellular functions for their own ends makes them highly effective pathogens and exquisite experimental tools. Work with viruses underpins much of our current understanding of molecular cell biology and related fields. Each of the eight chapters in this volume deals with a specific aspect of viral interactions with cellular membranes. These include chapters on viral entry, viral membrane fusion, viral membrane protein synthesis and transport, viral replication, viral interactions with cytoskeletal systems and the nucleus, the trafficking of viral membrane proteins and viral perturbation of host cell protein trafficking. These chapters should provide both an overview of cellular membrane trafficking mechanisms and viral interactions with these systems, as well as reviews of the current state of each of the fields.

A guide to modern scanning electron microscopy instrumentation, methodology and techniques, highlighting novel applications to cell and molecular biology.

This comprehensive account of the human herpesviruses provides an encyclopedic overview of their basic virology and clinical manifestations. This group of viruses includes human simplex type 1 and 2, Epstein–Barr virus, Kaposi's Sarcoma-associated herpesvirus, cytomegalovirus, HHV6A, 6B and 7, and varicella-zoster virus. The viral diseases and cancers they cause are significant and often recurrent. Their prevalence in the developed world accounts for a major burden of disease, and as a result there is a great deal of research into the pathophysiology of infection and immunobiology. Another important area covered within this volume concerns antiviral therapy and the development of vaccines. All these aspects are covered in depth, both scientifically and in terms of clinical guidelines for patient care. The text is illustrated generously throughout and is fully referenced to the latest research and developments.