Laser Tweezers in Cell Biology
Title | Laser Tweezers in Cell Biology PDF eBook |
Author | |
Publisher | Academic Press |
Pages | 241 |
Release | 1997-11-24 |
Genre | Science |
ISBN | 0080859534 |
Volume 55 in Methods in Cell Biology is a concise laboratory book that emphasizes the methods and technologies needed to use single polarized laser light source that functions simultaneously as an optical trap and a dual-beam interferometer.* * Provides a practical laboratory guide for methods and technologies used with laser tweezers* Includes comprehensive and easy-to-follow protocols
Laser Tweezers in Cell Biology
Title | Laser Tweezers in Cell Biology PDF eBook |
Author | Michael P. Sheetz |
Publisher | |
Pages | 228 |
Release | 1998 |
Genre | Cytology |
ISBN | 9780125641579 |
Volume 55 in Methods in Cell Biology is a concise laboratory book that emphasizes the methods and technologies needed to use single polarized laser light source that functions simultaneously as an optical trap and a dual-beam interferometer. * * Provides a practical laboratory guide for methods and technologies used with laser tweezers * Includes comprehensive and easy-to-follow protocols
Optical Tweezers
Title | Optical Tweezers PDF eBook |
Author | Philip H. Jones |
Publisher | Cambridge University Press |
Pages | 565 |
Release | 2015-12-03 |
Genre | Science |
ISBN | 1107051169 |
A comprehensive guide to the theory, practice and applications of optical tweezers, combining state-of-the-art research with a strong pedagogic approach.
Optical Tweezers
Title | Optical Tweezers PDF eBook |
Author | Arne Gennerich |
Publisher | Springer Nature |
Pages | 753 |
Release | 2022-09-05 |
Genre | Science |
ISBN | 1071622293 |
This detailed volume explores a wide variety of techniques involving optical tweezers, a technology that has become increasingly more accessible to a broad range of researchers. Beginning with recent technical advances, the book continues by covering the application of optical tweezers to study DNA-protein interactions and DNA motors, protocols to perform protein (un)folding experiments, the application of optical tweezers to study actin- and microtubule-associated motor proteins, and well as protocols for investigating the function and mechanical properties of microtubules and intermediate filaments, and more. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Optical Tweezers: Methods and Protocols, Second Edition serves as an ideal resource for expanding the accessibility and use of optical traps by scientists of diverse disciplines.
Micromanipulation by Light in Biology and Medicine
Title | Micromanipulation by Light in Biology and Medicine PDF eBook |
Author | Karl Otto Greulich |
Publisher | Springer Science & Business Media |
Pages | 308 |
Release | 2012-12-06 |
Genre | Science |
ISBN | 1461241103 |
There are probably few people who do not dream of the good old times, when do ing science often meant fascination, excitement, even adventure. In our time, do ing science involves often technology and, perhaps, even business. But there are still niches where curiosity and fascination have their place. The subject of this book, technological as its title may sound, is one of the fortunate examples. It will report on lasers generating the coldest places in the Universe, and on table top laser microtools which can produce a heat "inferno" as it prevails in the interior of the Sun, or simulate, for specific plant cells, microgravity of the space around our plan et Earth. There will be some real surprises for the reader. The applications range from basic studies of the driving forces of cell division (and thus life) via genetic modification of cells (for example, for plant breeding) to medical applications such as blood cell analysis and finally in vitro fertilization. What are these instruments: laser microbeams and optical tweezers? Both are lasers coupled with a fluorescence microscope. The laser microbeam uses a pulsed ultraviolet laser. Light is focused, as well as possible, in space and time, in order to obtain extremely high light intensities - high enough to generate, for a very short instant, extremely hot spots which can be used to cut, fuse or perforate biological material.
Methods in Cell Biology
Title | Methods in Cell Biology PDF eBook |
Author | Michael P. Sheetz |
Publisher | |
Pages | 228 |
Release | 1998 |
Genre | Lasers in cytology |
ISBN |
The Use of Optical Tweezers and Laser Scissors in Cell Biology
Title | The Use of Optical Tweezers and Laser Scissors in Cell Biology PDF eBook |
Author | Nicholas W. Hyun |
Publisher | |
Pages | 52 |
Release | 2014 |
Genre | |
ISBN | 9781303968563 |
Optical tweezers and laser scissors are invaluable tools to manipulate cells on the micro and nano scale level. By integrating laser technologies with imaging and biochemical techniques, robust systems can be created to study various biological and biochemical processes. The purpose of this thesis is to describe two different optical methods used to study two cell-based problems. In the first experiment, optical tweezers are used to examine the effects of viscosity on sperm motility and energetics using a 1064nm Nd:YVO4 continuous laser. In the second experiment, laser scissors are used to study growth cone response from laser induced damage using a Vanguard Nd: YVO4 second harmonic generator (SHG) 532 nm picosecond green laser. The proceeding sections describe the optical systems for each experiment as well as the methods used. Results of both studies are presented and discussed in detail.