The Histology Handbook is a clear, concise reference book that will provide the user with the information and understanding to assist in the performance and troubleshooting of histology related tasks and help us all succeed in our common pursuit of "the perfect slide". The book contains both basic information to facilitate the understanding of staining theory, as well as specific procedures that can be utilized in the histology laboratory. Additionally, there is a wealth of information regarding the troubleshooting of day to day common issues that can be the cause of sub-optimal results.

The examination and interpretation of tissue sections seen under the light microscope in a laboratory setting is an example of student-directed, independent problem solving. The proper reading of a histologic section is an acquired art that can only be developed through practice, close observation and repetition. This laboratory manual was designed as a guide for students to aid them in this endeavor. The laboratory study guide/manual was designed to be used as a supplement to any current textbook and/or atlas of Histology. Learning objectives provide the overall goals for each chapter. The narrative of the study guide explains how to systematically breakdown, examine and interpret each tissue and/or organ encountered, without regard to a given histologic slide from a specific slide collection. Thus, this systematic method can be used to examine and interpret histologic preparations from any collection or of any species. The student is encouraged to sketch, label and create a personalized atlas while using this laboratory manual as a guide. The vocabulary that should be developed and used during the laboratory can be found quickly by going to the bold face type in the appropriate segment of the text. Each chapter contains one or more tables in which key structures used in the identification of a tissue/organ are presented, offering the briefest possible summary of important histologic features. As a final short review, an appendix provides summary tables that compare and contrasts the basic differences of several structures that are somewhat similar in general architecture.

The second edition of this well-received book provides detailed information on the basic and advanced laboratory techniques in histopathology and cytology. It offers clear guidance on the principles and techniques of routine and special laboratory techniques. It also covers advanced laboratory techniques such as immunocytochemistry, flow cytometry, liquid-based cytology, polymerase chain reactions, tissue microarray, molecular technology, etc. The book's second edition covers several important recent topics with many new chapters, such as liquid biopsy, artificial neural network, digital pathology, and next-generation sequencing. Each chapter elucidates basic principle, practical methods, troubleshooting, and clinical applications of the technique. It includes multiple colored line drawings, microphotographs, and tables to illustrate each technique. The book is a helpful guide to the post-graduate students and fellows in pathology, practicing pathologists, as well as laboratory technicians, and research students.

Key features: High quality full color photographs and descriptive texts on the location and removal of the organs from the mouse Instructive methods and clear visuals for trimming and orienting the organs for paraffin histology to obtain the best possible sections for analysis Full color photomicrographs of the resulting section for each organ stained with hematoxylin and eosin demonstrating important features and landmarks for the histologist to ensure the optimal area for analysis is achieved All in one, easy to use guide organized by individual organs of the laboratory mouse Spiralbound for easy reference in the lab This "one-stop" guide offers an essential resource for any academic, research or development operation where mouse necropsy and/or histology are performed. Connecting the reader ‘from the mouse to the microscope’, it provides a detailed guide for locating, trimming, orientating and embedding of the most frequently investigated tissues collected in the laboratory mouse. It shows where the organs reside in the mouse, how to trim and embed them as well as the resulting optimal sections. This guide brings together the wealth of scattered information into one high-quality text, the emphasis is on providing knowledge that will help histologists and scientists get better results in any downstream assays where ideal sections are needed.