For the first time in over 20 years, a comprehensive collection of photographs and descriptions of species in the fungal genus Fusarium is available. This laboratory manual provides an overview of the biology of Fusarium and the techniques involved in the isolation, identification and characterization of individual species and the populations in which they occur. It is the first time that genetic, morphological and molecular approaches have been incorporated into a volume devoted to Fusarium identification. The authors include descriptions of species, both new and old, and provide protocols for genetic, morphological and molecular identification techniques. The Fusarium Laboratory Manual also includes some of the evolutionary biology and population genetics thinking that has begun to inform the understanding of agriculturally important fungal pathogens. In addition to practical “how-to” protocols it also provides guidance in formulating questions and obtaining answers about this very important group of fungi. The need for as many different techniques as possible to be used in the identification and characterization process has never been greater. These approaches have applications to fungi other than those in the genus Fusarium. This volume presents an introduction to the genus Fusarium, the toxins these fungi produce and the diseases they can cause. "The Fusarium Laboratory Manual is a milestone in the study of the genus Fusarium and will help bridge the gap between morphological and phylogenetic taxonomy. It will be used by everybody dealing with Fusarium in the Third Millenium." --W.F.O. Marasas, Medical Research Council, South Africa

This volume provides a collection of molecular protocols detailing the most common and modern techniques on fusarium wilt. Chapters guide readers through methods on initial isolation, molecular-based identification, genome characterization, generation of mutants, and characterization of interactions with other organisms including host plants. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Fusarium wilt: Methods and Protocols aims to be a valuable resource for mycologists, plant pathologists, microbiologists, geneticists, and other scientists that have an interest in members of the Fusarium oxysporum species complex or closely related fungi.

Contains a tribute to Paul E. Nelson, plus biographical information.

Fusarium species are ubiquitous environmental fungi and can cause severe invasive infections in plants. They are crop pathogens, and consumption of such infected crops can cause diseases in humans and animals. Furthermore, they act as spoilage organisms in stored products, such as wheat, sorghum, rice, and corn (maize). Fusarium species are mycotoxin producers and contaminate food and grains. Therefore, their eradication and management have economic importance as they can cause enormous economic and agricultural production losses. Despite the fact that the genus Fusarium Link (1809) has been known for over 200 years, new scientific information is being revealed by rapid advancements and breakthrough findings of interdisciplinary studies. This book presents an introductory overview of an update to the scientific knowledge about Fusarium. It discusses various aspects of Fusarium, such as its genetic diversity, root rot incidence and severity, genetic resistance, molecular markers, mycotoxins, diseases caused by Fusarium, and their management and the biological control of these phytopathogens. Furthermore, it also elaborates upon new plant secondary metabolites that are effective against Fusarium and the molecular interaction between Fusarium and the plant.

This 1984 book resulted from a symposium held by the British Mycological Society to bring together the many diverse areas of study of the genus Fusarium. Among the contributors to this volume were scientists working on applied and fundamental aspects of the subject. The book highlights the importance of the genus pathology, animal husbandry, human disease and as biodeteriogens. Yet other Fusarium spp. are sources of useful biochemicals: for example, one species is utilised for the manufacture of microbial protein for human consumption. All these topics are covered in this volume, which with continue to be of interest to a wide range of biologists and students.

The first book of its kind to focus on the diagnosis, prevention, and treatment of patients with fungal infections, this definitive reference returns in a completely revised, full-color new edition. It presents specific recommendations for understanding, controlling, and preventing fungal infections based upon underlying principles of epidemiology and infection control policy, pathogenesis, immunology, histopathology, and laboratory diagnosis and antifungal therapy. More than 560 photographs, illustrations, and tables depict conditions as they appear in real life and equip you to identify clinical manifestations with accuracy. Expanded therapy content helps you implement the most appropriate treatment quickly, and a bonus CD-ROM-featuring all of the images from the text-enables you to enhance your electronic presentations. Includes specific recommendations for diagnosing, preventing, and treating fungal infections in various patient populations based upon underlying principles of epidemiology and infection control policy, pathogenesis, immunology, histopathology, and laboratory diagnosis and antifungal therapy. Covers etiologic agents of disease, fungal infections in special hosts such as pediatric patients and patients with cancer and HIV, infections of specific organ systems, and more, to make you aware of the special considerations involved in certain cases. Features clinically useful and reader-friendly practical tools-including algorithms, slides, graphs, pictorials, photographs, and radiographs-that better illustrate and communicate essential points, promote efficient use in a variety of clinical and academic settings, and facilitate slide making for lectures and presentations. Offers a CD-ROM containing all of the book's images for use in your electronic presentations. Offers more clinically relevant images-more than 300 in full color for the first time-to facilitate diagnosis. Features expanded therapy-related content, including up-to-date treatment strategies and drug selection and dosing guidelines. Includes several new sections in the chapter on fungal infections in cancer patients that reflect the formidable clinical challenges these infections continue to present. Presents the work of additional international contributors who have defined many of the key issues in the field, providing more of a global perspective on the best diagnostic and management approaches. Uses a new, full-color design to enhance readability and ease of access to information.

Also included is a thorough review of the molecular genetics of both trichothecene and fumonisin biosynthesis, presenting more than 15 years of molecular biological research in an accessible form. Part one then reviews the natural occurrence and toxicity of agriculturally important mycotoxins, with historical case studies of suspected mycotoxicoses in humans and animals. These chapters also contain updates on the molecular genetics of additional mycotoxins and the importance of mycotoxins in plant diseases. This useful reference presents concise descriptions of mycotoxin-producing Fusarium species, as defined by the most recent concepts of fungal species biology and evolution. Each species' report includes a risk assessment based on its mycotoxin profile, occurrence in food and feed crops, and association with human and animal mycotoxicoses. Data on species distribution, mycotoxin profiles, and animal toxicity facilitate risk assessment for food and feed safety.