Diagnosis of Plant Virus Diseases presents a comprehensive summary of methods currently available for the diagnosis of plant diseases caused by viruses and viroids. Up-to-date literature references are provided, brief accounts of the basis for particular methods are included, and detailed protocols are presented. Procedures discussed include the use of host plants, electron microscopy of in vitro preparations, serological procedures (especially forms of ELISA, monoclonal antibodies, serological specific electron microscopy, and immunoblotting), and nucleic acid hybridization procedures. Strategies are outlined for implicating virus-like pathogens as causes of diseases of unknown etiology, and problems involved in identifying complexes of transmission-dependent and helper viruses are discussed. The book will be extremely useful for phytopathologists, plant virologists, and research students and workers in plant virology laboratories and diagnostic plant pathology laboratories.

Handbook of Plant Virus Diseases presents basic information about viral-caused and viral-like diseases in many cultivated crops. The editors, internationally known plant pathologists, provide authoritative descriptive symptomatic signatures of virus diseases, to aid in the diagnosis and possible control of viruses. This handbook organizes cultivated plants into groups according to their final destinations and uses after harvest-a useful grouping system that indicates that some diseases, their resultant epidemiology, and control measures are characteristic within different groups.

This edited book elucidates the evolution of plant virus, genomic structure, diversity, plant-virus interaction, subcellular movement etc. The book reviews the biological machineries which allow the emergence of virus populations adapted by plant. The main objective of this book is the demonstration of a clear synergistic effect of plant viruses, an effect that was unexpectedly as important as applied alone. Ornamental plants are very popular and economically important worldwide. The international market of ornamentals is constantly expanding. Viruses and viroids can significantly reduce both decorative value and quality of propagated material of ornamentals. Due to the wide range of ornamental plant species and cultivars and their wide geographical distribution, the diversity of viruses that infect them is also high. The new emerging viruses are the causal agent for the economic loss of many important ornamental plants. Therefore, this book also adds value to current knowledge of virus stress response in ornamental plants and will provide the groundwork necessary for building future strategies for product enhancement. This book is of interest to teachers, researchers, capacity builders and policymakers. It can serve as additional reading material for undergraduate and graduate students of virology, agriculture and plant sciences.

Using molecular methods for plant disease diagnosis provides diagnosticians with a number of advantages over more traditional methods. They can allow the identification of morphologically similar species, for example, or the detection of infection prior to symptom formation. Not only can molecular tools help by increasing the efficacy, accuracy and speed of diagnosis; their common technological basis provides further benefits, especially where resources are limited and traditional skills are hard to sustain. This book provides protocols for nucleic acid-based methods currently applied to plant pathogen detection and identification. It takes the practitioner through the full range of molecular diagnostic and detection methods and, as these generic techniques are appropriate for use on any target with minimal modification, also provides a useful resource for students of plant pathology and plant pathologists. Beginning with the background and future directions of the science, it then addresses DNA barcoding, microarrays, polymerase chain reactions (PCR), quality assurance and more, forming a complete reference on the subject.

Seeds provide an efficient means in disseminating plant virus and viroid diseases. The success of modern agriculture depends on pathogen free seed with high yielding character and in turn disease management. There is a serious scientific concern about the transmission of plant viruses sexually through seed and asexually through plant propagules. The present book provides the latest information along with the total list of seed transmitted virus and viroid diseases at global level including, the yield losses, diagnostic techniques, mechanism of seed transmission, epidemiology and virus disease management aspects. Additional information is also provided on the transmission of plant virus and virus-like diseases through vegetative propagules. It is also well known that seed transmitted viruses are introduced into new countries and continents during large-scale traffic movements through infected germplasm and plant propogules. The latest diagnostic molecular techniques in different virus-host combinations along with disease management measures have been included. The book shall be a good reference source and also a text book to the research scientists, teachers, students of plant pathology, agriculture, horticulture, life sciences, green house managers, professional entrepreneurs, persons involved in quarantines and seed companies. This book has several important features of seed transmitted virus diseases and is a good informative source and thus deserves a place in almost all university libraries, seed companies and research organizations.

Around the globe, besides fungal and bacterial diseases, both virus and viroid diseases have acquired greater importance in the realm of plant pathology and call for effective management measures as they are responsible for heavy yield losses and are a matter of vital importance and concern to farmers, horticulturists, gardeners and foresters. Understanding disease epidemiology is of vital importance for formulating viable disease management practices in a given agro-ecosystem. The development and progress of plant disease epidemics are variable from region to region. Epidemiology is not a static process, but rather a dynamic course that varies with a change in the ecology, host, vector and virus systems.

Plant virus and sub-viral pathogens pose severe constraints to the production of wide range of economically important crops worldwide. The crops raised both through true seed and vegetative propagated materials are affected with number of virus and virus-like diseases. The virus may enter into plants through seed planting materials or by vectors. Once the virus is in the field, it multiplies and spreads following definite patterns depending upon the nature of the vector and agro-meteorological conditions. Disease free crops and plants are great economic and social importance in feeding the world's population. Detection of virus and sub-viral agents at initial stages of infection is critical to reduce economic losses. For nearly two decades, ELISA and its variants played a major role in large scale virus testing and also in the production of virus-free planting materials.