Chromosome Microdissection and Cloning: A Practical Guide is a straightforward guide to chromosome microdissection and cloning. It presents an overview of the procedures and briefly reviews a few areas of research in which these techniques are applied. Topics range from preparation of chromosomes for microdissection to molecular cloning of microdissected chromosomal DNA. Methods of chromosome microdissection, including video microscope method and oil chamber method, are described. Comprised of five chapters, this book begins with an overview of the structure and organization of chromosomes, followed by a description of methods for preparing and preserving chromosomal DNA in a manner that is useful for cloning and direct analysis. Microdissection of metaphase chromosomes and isolation of fragments can be accomplished in one of the three ways described in the next chapter: by microdissection using an upright microscope and glass capillaries in an oil chamber; by laser microbeam; and with the use of an inverted microscope equipped with a video camera and high magnification-high resolution lenses. A step-by-step guide to these techniques and solutions for common problems are given following each method. Protocols for cloning and identifying genetic sequences from defined chromosome regions, particularly using the polymerase chain reaction, are also discussed. The final chapter focuses on applications of chromosome microdissection, such as cloning of disease-specific genes and generating "sequence tagged sites" to be used in large DNA sequencing projects. This monograph will be particularly helpful to investigators setting up microdissection systems de novo.

Defines the origin of ring extra structurally abnormal chromosomes (ESACs), relates the genetic content of different ring ESACs derived from the same chromosome with the patient's pheuotype, generates probes for diagnostic use and refines the critical region of Wolf-Hirschhorn syndrome.

What exactly is a gene? How does cloning actually work? Are designer babies a bad idea? Could we ever clone a human? The Rough Guide To Genes & Cloning answers all these questions and more. From the inside story of cells and their structure and the sleuths who cracked the genetic code to DNA cloning, twins and Dolly the sheep. Illustrated throughout with helpful pictures and diagrams, this Rough Guide turns the microscope on the things that make us what we are.

Several developmental and historical threads are woven and displayed in these two volumes of Bacterial Artificial Chromosomes, the first on Library Construction, Physical Mapping, and Sequencing, and the second on Fu- tional Studies. The use of large-insert clone libraries is the unifying feature, with many diverse contributions. The editors have had quite distinct roles. Shaying Zhao has managed several BAC end-sequencing projects. Marvin Stodolsky during 1970–1980 contributed to the elucidation of the natural b- teriophage/prophage P1 vector system. Later, he became a member of the Genome Task Group of the Department of Energy (DOE), through which s- port flowed for most clone library resources of the Human Genome Program (HGP). Some important historical contributions are not represented in this volume. This preface in part serves to mention these contributions and also briefly surveys historical developments. Leon Rosner (deceased) contributed substantially in developing a PAC library for drosophila that utilized a PI virion-based encapsidation and tra- fection process. This library served prominently in the Drosophila Genome Project collaboration. PACs proved easy to purify so that they substantially replaced the YACs used earlier. Much of the early automation for massive clone picking and processing was developed at the collaborating Lawrence Berkeley National Laboratory. However, the P1 virion encapsidation system itself was too fastidious, and P1 virion-based methods did not gain popularity in other genome projects.

The ability to successfully clone genes underlies the majority of our knowledge in molecular and cellular biology. Gene Cloning introduces the diverse array of techniques available to clone genes and how they can be used effectively both in the research laboratory, to gain knowledge about the gene, and for use in biotechnology, medicine, the pharmaceutical industry, and agriculture. It shows how cloning genes is an integral part of genomics and underlines its relevance in the post-genomic age, as a tool required to test predictions of gene regulation and function made through bioinformatics. Applications of gene cloning in medicine, both for diagnosis and treatment, and in the pharmaceutical industry and agriculture, are also covered in the book. Gene Cloning takes a fresh approach to teaching molecular and cellular biology and will be a valuable resource to both undergraduates and lecturers of biological and biomedical science courses.

Do You Realize How Much Impact DNA Technology has on Your Life Today? Registering your child's DNA with the police.bold new medical cures.the perfect tomato.gene cloning and DNA manipulation are no longer remote events that will have impact in your life - they are today's headlines! In this highly-acclaimed guide, Karl Drlica fully explains the basis of the ongoing genetic revolution. He guides you through the science and technology you need to understand the issues and make crucial decisions. Each step of the way he explains complex topics using easy-to-understand analogies. This basic information will help you: * Take advantage of the benefits emerging from the new genetics. * Protect yourself from the discrimination that may arise from release of genetic information. * Make informed political decisions about how much DNA technology will impact your life. "With the Genetic Revolution happening in the court rooms and doctors offices, this book is required reading for jurors, those concerned with genetic disease, or just the curious!"- Richard R. Sinden, Ph. D., Center for Genome Research, Texas A&M University "Successful investing in biotechnology requires knowledge of the science which drives it. Karl Drlica explains it in layman's terms."- Edward F. Tills, Second Vice President, Financial Consultant, Smith Barney, Inc. "The best text available to give the non-scientist or the scientist from a different field the necessary information to appreciate the implications of the latest genetic revolution."- Robert G. Fowler, Ph.D., San Jose University

We have made important progress since the beginning of the current grant year. We have further developed the microdissection and PCR- assisted microcloning techniques using the linker-adaptor method. We have critically evaluated the microdissection libraries constructed by this microtechnology and proved that they are of high quality. We further demonstrated that these microdissection clones are useful in identifying corresponding YAC clones for a thousand-fold expansion of the genomic coverage and for contig construction. We are also improving the technique of cloning the dissected fragments in test tube by the TDT method. We are applying both of these PCR cloning technique to human chromosomes 2 and 5 to construct region-specific libraries for physical mapping purposes of LLNL and LANL. Finally, we are exploring efficient procedures to use unique sequence microclones to isolate cDNA clones from defined chromosomal regions as valuable resources for identifying expressed gene sequences in the human genome. We believe that we are making important progress under the auspices of this DOE human genome program grant and we will continue to make significant contributions in the coming year. 4 refs., 4 figs.