Characterization of Hypoxia-inducible Factor-1Alpha (HIF-1Alpha) and Its Involvement in Oxygen-dependent Transferrin Expression
Title | Characterization of Hypoxia-inducible Factor-1Alpha (HIF-1Alpha) and Its Involvement in Oxygen-dependent Transferrin Expression PDF eBook |
Author | |
Publisher | |
Pages | 28 |
Release | 1997 |
Genre | |
ISBN |
Characterization of Hypoxia-inducible Factor-1α (HIF-1α)and Its Involvement in Oxygen-dependent Transferrin Expression
Title | Characterization of Hypoxia-inducible Factor-1α (HIF-1α)and Its Involvement in Oxygen-dependent Transferrin Expression PDF eBook |
Author | Andreas Rolfs |
Publisher | |
Pages | |
Release | 1997 |
Genre | |
ISBN |
Characterization of Hypoxia-inducible Factor-1[alpha] (HIF-1[alpha]) and Its Involvement in Oxygen-dependent Transferrin Expression
Title | Characterization of Hypoxia-inducible Factor-1[alpha] (HIF-1[alpha]) and Its Involvement in Oxygen-dependent Transferrin Expression PDF eBook |
Author | Andreas Rolfs |
Publisher | |
Pages | 28 |
Release | 1997 |
Genre | |
ISBN |
Characterization of Hypoxia-inducible Factor-1[alpha] (HIF-1[alpha]) and Its Involvemnet in Oxygen-dependent Transferrin Expression
Title | Characterization of Hypoxia-inducible Factor-1[alpha] (HIF-1[alpha]) and Its Involvemnet in Oxygen-dependent Transferrin Expression PDF eBook |
Author | Andreas Rolfs |
Publisher | |
Pages | 165 |
Release | 1997 |
Genre | |
ISBN |
Characterization of Hypoxia-inducible Factor-1a (HIF-1a) and Its Involvement in Oxygen-dependent Transferrin Expression
Title | Characterization of Hypoxia-inducible Factor-1a (HIF-1a) and Its Involvement in Oxygen-dependent Transferrin Expression PDF eBook |
Author | Andreas Rolfs |
Publisher | |
Pages | |
Release | 1997 |
Genre | |
ISBN |
Characterization of Hypoxia-inductible Factor-1@ (HIF-1) and Its Involvement in Oxygen-dependent Transferrin Expression
Title | Characterization of Hypoxia-inductible Factor-1@ (HIF-1) and Its Involvement in Oxygen-dependent Transferrin Expression PDF eBook |
Author | Andreas Rolfs |
Publisher | |
Pages | |
Release | 1997 |
Genre | |
ISBN |
Hypoxia-inducible Factor-1 Alpha (HIF-1alpha) and Its Role in the Hypoxic Preconditioning and Gene Expression Patterns in Zebrafish ( Danio Rerio) Embryos
Title | Hypoxia-inducible Factor-1 Alpha (HIF-1alpha) and Its Role in the Hypoxic Preconditioning and Gene Expression Patterns in Zebrafish ( Danio Rerio) Embryos PDF eBook |
Author | Amira F. Mohamed |
Publisher | |
Pages | 0 |
Release | 2007 |
Genre | |
ISBN |
Preconditioning to lowered oxygen levels (hypoxia) may occur when an animal is subjected to non-lethal levels of hypoxia and then returned to normoxic conditions. During a subsequent exposure to hypoxia, the pre-exposed animals may exhibit increased tolerance compared to naive animals. I have demonstrated, by analysis of critical PO2's (Pcrit), that zebrafish embryos can be preconditioned to hypoxia. Preconditioned embryos display a lower Pcrit than controls, indicating a heightened ability to endure hypoxic conditions. The role of the hypoxia inducible factor-1 (HIF-1) in promoting hypoxic preconditioning was examined. To determine the role of HIF-1 in preconditioning, embryos deficient in HIF-1alpha (using antisense oligonucleotide morpholinos) were assessed under hypoxic conditions. No significant difference between preconditioning capacities of control- and HIF-1alpha deficient embryos was observed. In addition to assessing Pcrit, a suite of hypoxia responsive genes were analysed by real time PCR. IGFBP-2 showed a significant decrease in expression in the HIF-1alpha deficient embryos, and EPO showed a significant increase in HIF-1alpha deficient embryos. All other genes examined showed no significant change between treated and control embryos.