Cellular and Molecular Approaches in Fish Biology is a highly interdisciplinary resource to bring industry professionals, students and researchers up-to-date with the latest developments and information on fish biology research combining a historical overview of the different research areas in fish biology and detailed descriptions of cellular and molecular approaches with explanations and recommendations for research. The book presents a global perspective of each research area with detailed analytical methodologies on the cellular and molecular mechanisms within fish biology for expermentation. The book provides different points of view on how researchers have addressed timely issues, while describing and dissecting some of the new experimental/analytical approaches used to answer the key questions at cellular and molecular levels, making this a valuable resource to those in industry and academia as well as those entering the field. - Provides detailed descriptions of each research approach, highlighting the tricks of the trade for its effective and successful application - Includes the latest developments in fish reproduction, fish development and nutrition, fish welfare, fish immunology, ecology and biomedics - Presents hot topics of research such as genetics, transcriptomics and epigenetics

Many physiological processes are regulated by the movement of ions into and out of organs, tissues, and cells. During the past decade, a variety of new techniques and approaches have contributed to a deeper understanding of the myriad influences ions have on the function and structure of organisms. From respiration and excretion to neurological control and metabolic processing, ions and their regulation occupy a central role in physiology of fish as well as other organisms. - Comprehensive update of ionic regulation in fish - Focuses on wide variety of organ systems and the influence of ions on organ system function - Contributions from an international group

The past several decades have witnessed an impressive array of conceptual and techno logical advances in the biomedical sciences. Much of the progress in this area has developed directly as a result of new morphology-based methods that have permitted the assessment of chemical, enzymatic, immunological, and molecular parameters at the cellular and tissue levels. Additional novel approaches including laser capture microdissection have also emerged for the acquisition of homogeneous cell popula tions for molecular analyses. These methodologies have literally reshaped the approaches to fundamental biological questions and have also had a major impact in the area of diagnostic pathology. Much of the groundwork for the development of morphological methods was estab th lished in the early part of the 19 century by Francois-Vincent Raspail, generally acknowledged as the founder of the science of histochemistry. The earliest work in the field was primarily in the hands of botanists and many of the approaches to the under standing of the chemical composition of cells and tissues involved techniques such as microincineration, which destroyed structural integrity. The development of aniline th dyes in the early 20 century served as a major impetus to studies of the structural rather than chemical composition of tissue. Later in the century, however, the focus returned to the identification of chemical constituents in the context of intact cell and tissue structure.

The zebrafish is the most important fish model in developmental andgenetic analyses. This book contains 19 review articles covering abroad spectrum of topics, from development to genetic tools. Thecontents range from early development, the role of maternal factorsand gastrulation, to tissue differentiation and organogenesis, such asdevelopment of the organizer, notochord, floor plate, nervous system, somites, muscle, skeleton and endoderm

The book presents updated information on the cryopreservation of semen, embryos, germ cells and ovarian follicles of neotropical fish from South America. The chapters address the importance of developing germplasm banks of South American fish, both for commercial production and for conservation of ichthyofauna. This book has been designed mainly to provide a historical review of the 40 years of reproductive cryobiology research on South American fish species. The work is aimed at students, researchers and technicians involved in the areas related to the book's theme. The editors invite the readers to delve into dense technical knowledge, developed by renowned researchers.

Gills of healthy fishes are their lifeline to meet the challenges arising from their changing environment: oxygen gradient, alkalinity, temperature fluctuations and the added pollutants. The diverse and ever changing aquatic environment has a major impact on the organization of various organ-systems of fishes. This book contains seventeen chapters

For the first time, here is a book that focuses on in vitro approaches to the study of the toxicology of polluting agents (including heavy metals, radionuclides, micro-organics, estrogenic compounds, and complex mixtures) in the aquatic environment. The importance of in vitro methods is that they allow standardised techniques to be developed and validated for substance and species specific experiments in a controlled way. Also, they allow mechanistic studies without the problems of individual variation between animals and environmental stress.