This book is open access under a CC BY-NC 2.5 license. This book offers 19 detailed protocols on the use of induced mutations in crop breeding and functional genomics studies, which cover topics including chemical and physical mutagenesis, phenotypic screening methods, traditional TILLING and TILLING by sequencing, doubled haploidy, targeted genome editing, and low-cost methods for the molecular characterization of mutant plants that are suitable for laboratories in developing countries. The collection of protocols equips users with the techniques they need in order to start a program on mutation breeding or functional genomics using both forward and reverse-genetic approaches. Methods are provided for seed and vegetatively propagated crops (e.g. banana, barley, cassava, jatropha, rice) and can be adapted for use in other species.

Biotechnology and Plant Breeding includes critical discussions of the newest and most important applications of biotechnology in plant breeding, covering key topics such as biometry applied to molecular analysis of genetic diversity, genetically modified plants, and more. This work goes beyond recombinant DNA technology to bring together key information and references on new biotech tools for cultivar development, such as double-haploids, molecular markers, and genome-wide selection, among others. It is increasingly challenging for plant breeders and agricultural systems to supply enough food, feed, fiber and biofuel for the global population. As plant breeding evolves and becomes increasingly sophisticated, a staggering volume of genetic data is now generated. Biotechnology and Plant Breeding helps researchers and students become familiar with how the vast amounts of genetic data are generated, stored, analyzed and applied. This practical resource integrates information about plant breeding into the context of modern science, and assists with training for plant breeders including those scientists who have a good understanding of molecular biology/biotechnology and need to learn the art and practice of plant breeding. Plant biologists, breeding technicians, agronomists, seed technologists, students, and any researcher interested in biotechnologies applied to plant breeding will find this work an essential tool and reference for the field. - Presents in-depth but easy-to-understand coverage of topics, so plant breeders can readily comprehend them and apply them to their breeding programs - Includes chapters that address the already developed and optimized biotechnologies for cultivar development, with real-world application for users - Features contributions by authors with several years of experience in their areas of expertise

As the oldest and largest human intervention in nature, the science of agriculture is one of the most intensely studied practices. From manipulation of plant gene structure to the use of plants for bioenergy, biotechnology interventions in plant and agricultural science have been rapidly developing over the past ten years with immense forward leaps on an annual basis. This book begins by laying the foundations for plant biotechnology by outlining the biological aspects including gene structure and expression, and the basic procedures in plant biotechnology of genomics, metabolomics, transcriptomics and proteomics. It then focuses on a discussion of the impacts of biotechnology on plant breeding technologies and germplasm sustainability. The role of biotechnology in the improvement of agricultural traits, production of industrial products and pharmaceuticals as well as biomaterials and biomass provide a historical perspective and a look to the future. Sections addressing intellectual property rights and sociological and food safety issues round out the holistic discussion of this important topic. Includes specific emphasis on the inter-relationships between basic plant biotechnologies and applied agricultural applications, and the way they contribute to each other Provides an updated review of the major plant biotechnology procedures and techniques, their impact on novel agricultural development and crop plant improvement Takes a broad view of the topic with discussions of practices in many countries

Written in easy to follow language, the book presents cutting-edge agriculturally relevant plant biotechnologies and applications in a manner that is accessible to all. This book introduces the scope and method of plant biotechnologies and molecular breeding within the context of environmental analysis and assessment, a diminishing supply of productive arable land, scarce water resources and climate change. Authors who have studied how agro ecosystems have changed during the first decade and a half of commercial deployment review effects and stress needs that must be considered to make these tools sustainable.

"The book...is, in fact, a short text on the many practical problems...associated with translating the explosion in basic biotechnological research into the next Green Revolution," explains Economic Botany. The book is "a concise and accurate narrative, that also manages to be interesting and personal...a splendid little book." Biotechnology states, "Because of the clarity with which it is written, this thin volume makes a major contribution to improving public understanding of genetic engineering's potential for enlarging the world's food supply...and can be profitably read by practically anyone interested in application of molecular biology to improvement of productivity in agriculture."

Abstract: This book presents contemporary information on mutagenesis in plants and its applications in plant breeding and research. The topics are classified into sections focusing on the concepts, historical development and genetic basis of plant mutation breeding (chapters 1-6); mutagens and induced mutagenesis (chapters 7-13); mutation induction and mutant development (chapters 14-23); mutation breeding (chapters 24-34); or mutations in functional genomics (chapters 35-41). This book is an essential reference for those who are conducting research on mutagenesis as an approach to improving or modifying a trait, or achieving basic understanding of a pathway for a trait --.