Alternative Approaches in Median Lethality (LD50) and Acute Toxicity Testing
Title | Alternative Approaches in Median Lethality (LD50) and Acute Toxicity Testing PDF eBook |
Author | Linval R. DePass |
Publisher | |
Pages | 12 |
Release | 1990 |
Genre | |
ISBN |
The LD50 test was introduced by Trevan in 1927 (1) for biological standardization of dangerous drugs. Since then, the LD50 has gained wide acceptance as a measure of acute toxicity of all types of substances. Recently, however, the LD50 test has been criticized as an unnecessary waste of resources. Therefore, efforts have been made to reduce the number of animals used in such tests and to avoid using this test unless required by regulations. A review of the literature has shown that a relatively small number of animals per dose level (5) and a small number of dose levels (2 or 3) are usually sufficient to calculate an LD50 and slope using moving average methods. In addition, one sex should suffice because large sex differences are seldom encountered. When a formal LD50 is not required, one of several approximate methods may be used to estimate the lethal dose. Further approaches include in vitro cytotoxicity methods and computer-based structure-activity models. The in vitro methods are still in a nearly stage of development and will require extensive validation before they are accepted by the toxicology community. In conclusion, when LD50 tests are required, the most economical approach should be used, without undue concern for statistical precision.
The LD50 (median Lethal Dose) and LC50 (median Lethal Concentration) Toxicity Tests
Title | The LD50 (median Lethal Dose) and LC50 (median Lethal Concentration) Toxicity Tests PDF eBook |
Author | Michael D. Kreger |
Publisher | |
Pages | 62 |
Release | 1992 |
Genre | Toxicity testing |
ISBN |
The LD50 (median Lethal Dose) and LC50 (median Lethal Concentration) Toxicity Tests, January 1980 - August 1990
Title | The LD50 (median Lethal Dose) and LC50 (median Lethal Concentration) Toxicity Tests, January 1980 - August 1990 PDF eBook |
Author | Karen J. Clingerman |
Publisher | |
Pages | 62 |
Release | 1990 |
Genre | Alternative toxicity testing |
ISBN |
Acute Toxicity Testing
Title | Acute Toxicity Testing PDF eBook |
Author | Alan M. Goldberg |
Publisher | |
Pages | 312 |
Release | 1984 |
Genre | Medical |
ISBN |
The LD50 (median Lethal Dose) Toxicity Test, 1980-1988
Title | The LD50 (median Lethal Dose) Toxicity Test, 1980-1988 PDF eBook |
Author | Karen J. Clingerman |
Publisher | |
Pages | 36 |
Release | 1989 |
Genre | Government publications |
ISBN |
Alternative Methods for Toxicity Testing
Title | Alternative Methods for Toxicity Testing PDF eBook |
Author | |
Publisher | |
Pages | 192 |
Release | 1986 |
Genre | Animal experimentation |
ISBN |
Test No. 223: Avian Acute Oral Toxicity Test
Title | Test No. 223: Avian Acute Oral Toxicity Test PDF eBook |
Author | OECD |
Publisher | OECD Publishing |
Pages | 25 |
Release | 2010-07-23 |
Genre | |
ISBN | 9264090894 |
This Test Guideline describes procedures designed to estimate the acute oral toxicity of substances to birds, and it provides three testing options: (1) limit dose test, (2) LD50-slope test, and (3) LD50-only test. The LD50-slope and LD50-only ...