The Genus Citrus presents the enormous amount of new knowledge that has been generated in recent years on nearly all topics related to citrus. Beginning with an overview of the fundamental principles and understanding of citrus biology and behavior, the book provides a comprehensive view from Citrus evolution to current market importance. Reporting on new insights supported by the elucidation of the citrus genome sequence, it presents groundbreaking theories and fills in previous knowledge gaps. Because citrus is among the most difficult plants to improve through traditional breeding, citrus researchers, institutions and industries must quickly learn to adapt to new developments, knowledge and technologies to address the biological constraints of a unique fruit-tree such as citrus. Despite the challenges of working with citrus, tremendous progress has been made, mostly through advances in molecular biology and genomics. This book is valuable for all those involved with researching and advancing, producing, processing, and delivering citrus products. Includes the most current research on citrus genomic information Provides the first detailed description of citrus origin, a new proposal for citrus taxonomy, and a redefinition of the genus Citrus Details citrus challenges including climate change, global disease impacts, and plant improvement strategies

With 1 page corrigendum

Plant viruses grouped within this family have remarkable properties, prominent among which is their genomic size: Citrus tristeza virus (CTV) has the largest (19.3 kb) genome reported for a plant monopartite single-stranded RNA (+) virus. Virions are filamentous and typically flexuous particles, approximately 12 nm in diameter and 650 to 2000 nm in length, with a unique bipolar (“rattlesnake”) morphology: the major coat protein (CP) encapsidates most of the genomic RNA, with a minor CP (CPm) coating a small 5’-terminal fragment (virion tail) and other viral-encoded proteins being also incorporated to this tail. The genome is monopartite (genus Closterovirus, type member Beet yellows virus, and genus Ampelovirus, type member Grapevine leafroll-associated virus 3) or bipartite (genus Crinivirus, type member Lettuce infectious yellows virus, with at least one example of tripartite genome). The genomic RNA (or RNA1 in criniviruses) directs translation of the two 5’-proximal ORFs (via a peculiar ribosomal frameshift mechanism and proteolytic processing) that encode replication-related components, with the 3’-proximal ORFs encoding proteins expressed from 3’-coterminal subgenomic RNAs. A genomic signature of members of the family Closteroviridae is the presence of a five-gene block of proteins involved in virion assembly and movement that, in addition to the CP and CPm, includes a small transmembrane protein, a homologue of the HSP70 class of heat-shock proteins and a diverged CP. Members of this family encode suppressors of RNA silencing differing in number (up to three in CTV), and in mode of action: intracellular, intercellular, or both. In this same context Sweet potato chlorotic stunt virus codes for a singular suppressor: an RNase III that catalyzes cleavage of the small interfering RNAs mediating RNA silencing. Host range is usually narrow and, in order to expand it, some member(s) of the family, illustrated by the case of CTV, have evolved by acquiring multiple non-conserved genes. Virion accumulation is restricted to the phloem, with aphids, mealybugs and whiteflies (depending on the genus) operating as natural vectors. Disease symptoms may be expressed in leaves, fruits and trunk of the woody hosts. Natural Plant viruses grouped within this family have remarkable properties, prominent among which is their genomic size: Citrus tristeza virus (CTV) has the largest (19.3 kb) genome reported for a plant monopartite single-stranded RNA (+) virus. Virions are filamentous and typically flexuous particles, approximately 12 nm in diameter and 650 to 2000 nm in length, with a unique bipolar (“rattlesnake”) morphology: the major coat protein (CP) encapsidates most of the genomic RNA, with a minor CP (CPm) coating a small 5’-terminal fragment (virion tail) and other viral-encoded proteins being also incorporated to this tail. The genome is monopartite (genus Closterovirus, type member Beet yellows virus, and genus Ampelovirus, type member Grapevine leafroll-associated virus 3) or bipartite (genus Crinivirus, type member Lettuce infectious yellows virus, with at least one example of tripartite genome). The genomic RNA (or RNA1 in criniviruses) directs translation of the two 5’-proximal ORFs (via a peculiar ribosomal frameshift mechanism and proteolytic processing) that encode replication-related components, with the 3’-proximal ORFs encoding proteins expressed from 3’-coterminal subgenomic RNAs. A genomic signature of members of the family Closteroviridae is the presence of a five-gene block of proteins involved in virion assembly and movement that, in addition to the CP and CPm, includes a small transmembrane protein, a homologue of the HSP70 class of heat-shock proteins and a diverged CP. Members of this family encode suppressors of RNA silencing differing in number (up to three in CTV), and in mode of action: intracellular, intercellular, or both. In this same context Sweet potato chlorotic stunt virus codes for a singular suppresso.

This book is intended to provide consolidated information on citrus breeding in the era of biotechnology, which is likely to hasten the pace of variety development aimed at resolving the problems faced by grove owners growing currently available cultivars. The subjects covered are focused on citrus while providing information equally useful to the breeders of other tree crops. It will also help students of genetic and breeding identify appropriate applications of biotechnology in citrus breeding. While providing information on future avenues, it also reviews the past progress and achievements ensuring continuity of the subject. Several chapters include protocols for novel techniques that should facilitate their broader application by citrus breeders.